28308 Search Results


283 mycobacterium porcinum  (ATCC)
92
ATCC 283 mycobacterium porcinum
283 Mycobacterium Porcinum, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/283 mycobacterium porcinum/product/ATCC
Average 92 stars, based on 1 article reviews
283 mycobacterium porcinum - by Bioz Stars, 2026-03
92/100 stars
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mouse anti human monoclonal e2f3  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse anti human monoclonal e2f3
Figure 3. <t>E2F3</t> is a direct target of miR‑433 in non‑small‑cell lung cancer. (A) Putative Wt and Mut binding sequences in the 3'‑UTR of E2F3. (B) A549 and H460 cells were cotransfected with miR‑433 mimics or miR‑NC and pMIR‑Wt‑E2F3‑3'‑UTR or pMIR‑Mut‑E2F3‑3'‑UTR. Following transfection for 48 h, relative luciferase activity was determined using a dual luciferase reporter assay system. *P<0.05 vs. miR‑NC. E2F3 (C) mRNA and (D) protein expression levels were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively, in A549 and H460 cells transfected with miR‑433 mimics or miR‑NC. *P<0.05 vs. miR‑NC. E2F3, E2F transcription factor 3; miR, microRNA; Mut, mutant; NC, negative control; pMIR, plasmid vector; UTR, untranslated region; Wt, wild‑type.
Mouse Anti Human Monoclonal E2f3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti human monoclonal e2f3/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
mouse anti human monoclonal e2f3 - by Bioz Stars, 2026-03
93/100 stars
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grinder philips hr 28308/b  (Philips Healthcare)
90
Philips Healthcare grinder philips hr 28308/b
Figure 3. <t>E2F3</t> is a direct target of miR‑433 in non‑small‑cell lung cancer. (A) Putative Wt and Mut binding sequences in the 3'‑UTR of E2F3. (B) A549 and H460 cells were cotransfected with miR‑433 mimics or miR‑NC and pMIR‑Wt‑E2F3‑3'‑UTR or pMIR‑Mut‑E2F3‑3'‑UTR. Following transfection for 48 h, relative luciferase activity was determined using a dual luciferase reporter assay system. *P<0.05 vs. miR‑NC. E2F3 (C) mRNA and (D) protein expression levels were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively, in A549 and H460 cells transfected with miR‑433 mimics or miR‑NC. *P<0.05 vs. miR‑NC. E2F3, E2F transcription factor 3; miR, microRNA; Mut, mutant; NC, negative control; pMIR, plasmid vector; UTR, untranslated region; Wt, wild‑type.
Grinder Philips Hr 28308/B, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/grinder philips hr 28308/b/product/Philips Healthcare
Average 90 stars, based on 1 article reviews
grinder philips hr 28308/b - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Figure 3. E2F3 is a direct target of miR‑433 in non‑small‑cell lung cancer. (A) Putative Wt and Mut binding sequences in the 3'‑UTR of E2F3. (B) A549 and H460 cells were cotransfected with miR‑433 mimics or miR‑NC and pMIR‑Wt‑E2F3‑3'‑UTR or pMIR‑Mut‑E2F3‑3'‑UTR. Following transfection for 48 h, relative luciferase activity was determined using a dual luciferase reporter assay system. *P<0.05 vs. miR‑NC. E2F3 (C) mRNA and (D) protein expression levels were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively, in A549 and H460 cells transfected with miR‑433 mimics or miR‑NC. *P<0.05 vs. miR‑NC. E2F3, E2F transcription factor 3; miR, microRNA; Mut, mutant; NC, negative control; pMIR, plasmid vector; UTR, untranslated region; Wt, wild‑type.

Journal: Molecular medicine reports

Article Title: MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3.

doi: 10.3892/mmr.2018.9020

Figure Lengend Snippet: Figure 3. E2F3 is a direct target of miR‑433 in non‑small‑cell lung cancer. (A) Putative Wt and Mut binding sequences in the 3'‑UTR of E2F3. (B) A549 and H460 cells were cotransfected with miR‑433 mimics or miR‑NC and pMIR‑Wt‑E2F3‑3'‑UTR or pMIR‑Mut‑E2F3‑3'‑UTR. Following transfection for 48 h, relative luciferase activity was determined using a dual luciferase reporter assay system. *P<0.05 vs. miR‑NC. E2F3 (C) mRNA and (D) protein expression levels were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively, in A549 and H460 cells transfected with miR‑433 mimics or miR‑NC. *P<0.05 vs. miR‑NC. E2F3, E2F transcription factor 3; miR, microRNA; Mut, mutant; NC, negative control; pMIR, plasmid vector; UTR, untranslated region; Wt, wild‑type.

Article Snippet: The primary antibodies used in the present study were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and included mouse anti-human monoclonal E2F3 (1:1,000; cat. no. sc-28308) and mouse anti-human monoclonal GAPDH (1:1,000; cat. no. sc-365062) antibodies.

Techniques: Binding Assay, Transfection, Luciferase, Activity Assay, Reporter Assay, Expressing, Polymerase Chain Reaction, Western Blot, Mutagenesis, Negative Control, Plasmid Preparation

Figure 4. E2F3 downregulation suppresses cell proliferation and invasion in non‑small‑cell lung cancer. (A) E2F3 siRNA or NC siRNA was transfected into A549 and H460 cells. A total of 72 h post‑transfection, western blot analysis was performed to detect E2F3 protein expression levels. *P<0.05 vs. NC siRNA. The effect of E2F3 knockdown on A549 and H460 cell proliferation and invasion was determined by a (B) Cell Counting kit‑8 and (C) cell invasion assays, respectively (magnification, x200). *P<0.05 vs. NC siRNA. E2F3, E2F transcription factor 3; NC, negative control; OD, optical density; siRNA, small interfering RNA.

Journal: Molecular medicine reports

Article Title: MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3.

doi: 10.3892/mmr.2018.9020

Figure Lengend Snippet: Figure 4. E2F3 downregulation suppresses cell proliferation and invasion in non‑small‑cell lung cancer. (A) E2F3 siRNA or NC siRNA was transfected into A549 and H460 cells. A total of 72 h post‑transfection, western blot analysis was performed to detect E2F3 protein expression levels. *P<0.05 vs. NC siRNA. The effect of E2F3 knockdown on A549 and H460 cell proliferation and invasion was determined by a (B) Cell Counting kit‑8 and (C) cell invasion assays, respectively (magnification, x200). *P<0.05 vs. NC siRNA. E2F3, E2F transcription factor 3; NC, negative control; OD, optical density; siRNA, small interfering RNA.

Article Snippet: The primary antibodies used in the present study were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and included mouse anti-human monoclonal E2F3 (1:1,000; cat. no. sc-28308) and mouse anti-human monoclonal GAPDH (1:1,000; cat. no. sc-365062) antibodies.

Techniques: Transfection, Western Blot, Expressing, Knockdown, CCK-8 Assay, Negative Control, Small Interfering RNA

Figure 5. E2F3 restoration counteracts the effects of miR‑433 on non‑small‑cell lung cancer cell proliferation and invasion. A549 and H460 cells were trans fected with miR‑433 mimics with pcDNA3.1‑E2F3 or pcDNA3.1, or miR‑NC alone. (A) Western blot analysis was employed to detect E2F3 protein expression levels at 72 h following transfection. *P<0.05 vs. miR‑NC. #P<0.05 vs. miR‑433 mimics+pcDNA3.1‑E2F3. (B) Cell Counting kit‑8 and (C) cell invasion assays were applied to determine the proliferation and invasion in variably treated cells. *P<0.05 vs. miR‑NC. #P<0.05 vs. miR‑433 mimics+pcDNA3.1‑E2F3 (magnification, x200). E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; pcDNA3.1, plasmid vector; OD, optical density.

Journal: Molecular medicine reports

Article Title: MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3.

doi: 10.3892/mmr.2018.9020

Figure Lengend Snippet: Figure 5. E2F3 restoration counteracts the effects of miR‑433 on non‑small‑cell lung cancer cell proliferation and invasion. A549 and H460 cells were trans fected with miR‑433 mimics with pcDNA3.1‑E2F3 or pcDNA3.1, or miR‑NC alone. (A) Western blot analysis was employed to detect E2F3 protein expression levels at 72 h following transfection. *P<0.05 vs. miR‑NC. #P<0.05 vs. miR‑433 mimics+pcDNA3.1‑E2F3. (B) Cell Counting kit‑8 and (C) cell invasion assays were applied to determine the proliferation and invasion in variably treated cells. *P<0.05 vs. miR‑NC. #P<0.05 vs. miR‑433 mimics+pcDNA3.1‑E2F3 (magnification, x200). E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; pcDNA3.1, plasmid vector; OD, optical density.

Article Snippet: The primary antibodies used in the present study were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and included mouse anti-human monoclonal E2F3 (1:1,000; cat. no. sc-28308) and mouse anti-human monoclonal GAPDH (1:1,000; cat. no. sc-365062) antibodies.

Techniques: Western Blot, Expressing, Transfection, CCK-8 Assay, Negative Control, Plasmid Preparation