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Image Search Results
Journal: Molecular medicine reports
Article Title: Recombinant chromosome 6 open reading frame 120 protein promotes angiogenesis and endothelial‑to‑mesenchymal transition in human umbilical vein endothelial cells via the PI3K/Akt signaling pathway.
doi: 10.3892/mmr.2025.13596
Figure Lengend Snippet: Figure 2. rC6ORF120 induced the EndMT in HUVECs. (A) Representative western blot images of endothelial (CD31 and VE‑cadherin) and mesenchymal (Vimentin and α‑SMA) marker proteins in HUVECs following treatment with rC6ORF120 and TGF‑β1. (B‑E) Corresponding bar graph representing rela tive expression of protein expression levels, normalized to GAPDH. *P<0.05; **P<0.01; ***P<0.001. rC6ORF120, recombinant C6ORF120 protein; EndMT, endothelial‑mesenchymal transition; HUVECs, human umbilical vein endothelial cells; α‑SMA, α‑smooth muscle actin.
Article Snippet: After blocking with 5% skimmed milk for 1 h at room temperature, membranes were incubated overnight at 4 ̊C with primary
Techniques: Western Blot, Marker, Expressing, Recombinant
Journal: Molecular medicine reports
Article Title: Recombinant chromosome 6 open reading frame 120 protein promotes angiogenesis and endothelial‑to‑mesenchymal transition in human umbilical vein endothelial cells via the PI3K/Akt signaling pathway.
doi: 10.3892/mmr.2025.13596
Figure Lengend Snippet: Figure 3. rC6ORF120 alters the fluorescence intensity of EndMT markers. Immunocytofluorescence staining of (A) α‑SMA and (C) CD31 in HUVECs following rC6ORF120 treatment, with (B and D) corresponding statistical analysis. **P<0.01; ***P<0.001. Scale bar, 200 µm. rC6ORF120, recombinant C6ORF120 protein; EndMT, endothelial‑mesenchymal transition; HUVECs, human umbilical vein endothelial cells.
Article Snippet: After blocking with 5% skimmed milk for 1 h at room temperature, membranes were incubated overnight at 4 ̊C with primary
Techniques: Fluorescence, Staining, Recombinant
Journal: Molecular medicine reports
Article Title: Recombinant chromosome 6 open reading frame 120 protein promotes angiogenesis and endothelial‑to‑mesenchymal transition in human umbilical vein endothelial cells via the PI3K/Akt signaling pathway.
doi: 10.3892/mmr.2025.13596
Figure Lengend Snippet: Figure 7. Inhibition of the PI3K/Akt pathway reverses the induction of EndMT by rC6ORF120. Immunofluorescence analysis of (A) α‑SMA and (C) CD31. The bar graphs depict the relative fluorescence intensity of (B) α‑SMA and (D) CD31. ns indicates no statistically significant difference; ***P<0.001. Scale bar, 200 µm. EndMT, endothelial‑mesenchymal transition; rC6ORF120, recombinant C6ORF120 protein; α‑SMA, α‑smooth muscle actin.
Article Snippet: After blocking with 5% skimmed milk for 1 h at room temperature, membranes were incubated overnight at 4 ̊C with primary
Techniques: Inhibition, Immunofluorescence, Fluorescence, Recombinant