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Proteintech opa1
Effects of EA treatment on the expressions of Mfn2, <t>OPA1,</t> and Drp1 in the prefrontal cortex. ( A – D ) Western blotting for expression levels of Mfn2, OPA1, and Drp1 in the prefrontal cortical tissues. ( E – G ) qRT-PCR for expression levels of Mfn2, OPA1, and Drp1 in prefrontal cortical tissues. Sham vs PSD, **p < 0.01; PSD vs EA, # p < 0.05, ## p < 0.01, PSD vs CC, & p < 0.05, CC vs EA + CC, ^ p < 0.05.
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Effects of EA treatment on the expressions of Mfn2, OPA1, and Drp1 in the prefrontal cortex. ( A – D ) Western blotting for expression levels of Mfn2, OPA1, and Drp1 in the prefrontal cortical tissues. ( E – G ) qRT-PCR for expression levels of Mfn2, OPA1, and Drp1 in prefrontal cortical tissues. Sham vs PSD, **p < 0.01; PSD vs EA, # p < 0.05, ## p < 0.01, PSD vs CC, & p < 0.05, CC vs EA + CC, ^ p < 0.05.

Journal: Neuropsychiatric Disease and Treatment

Article Title: Electroacupuncture Ameliorates Depression-Like Behaviors in Post-Stroke Rats via Activating AMPK-Mediated Mitochondrial Function

doi: 10.2147/NDT.S436177

Figure Lengend Snippet: Effects of EA treatment on the expressions of Mfn2, OPA1, and Drp1 in the prefrontal cortex. ( A – D ) Western blotting for expression levels of Mfn2, OPA1, and Drp1 in the prefrontal cortical tissues. ( E – G ) qRT-PCR for expression levels of Mfn2, OPA1, and Drp1 in prefrontal cortical tissues. Sham vs PSD, **p < 0.01; PSD vs EA, # p < 0.05, ## p < 0.01, PSD vs CC, & p < 0.05, CC vs EA + CC, ^ p < 0.05.

Article Snippet: After blocking with 5% nonfat milk at 20–25°C for 2h, samples were incubated overnight at 4°C with primary antibodies against AMPK (1:1000, Abcam, ab27118), p-AMPK (1:1000, CST, 2535S), Drp1 (1:5000, Proteintech, 12957-1-AP), Mfn2 (1:5000, Proteintech, 12186-1-AP), OPA1 (1:4000, Proteintech, 27722-1-AP), and GAPDH (1:5000, Proteintech, 10494-1-AP).

Techniques: Western Blot, Expressing, Quantitative RT-PCR