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2 oxoadipate nad coa glutaryl coa nadh h co2  (Enamine Ltd)
88
Enamine Ltd 2 oxoadipate nad coa glutaryl coa nadh h co2
2 Oxoadipate Nad Coa Glutaryl Coa Nadh H Co2, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facultative anaerobic bacteria escherichia coli jm109 19 86 escherichia coli nm522 28 22 escherichia coli xl 1 blue 26 95 serratia marcescens atcc  (ATCC)
90
ATCC facultative anaerobic bacteria escherichia coli jm109 19 86 escherichia coli nm522 28 22 escherichia coli xl 1 blue 26 95 serratia marcescens atcc
Facultative Anaerobic Bacteria Escherichia Coli Jm109 19 86 Escherichia Coli Nm522 28 22 Escherichia Coli Xl 1 Blue 26 95 Serratia Marcescens Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/facultative anaerobic bacteria escherichia coli jm109 19 86 escherichia coli nm522 28 22 escherichia coli xl 1 blue 26 95 serratia marcescens atcc/product/ATCC
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anti oga antibody  (Proteintech)
93
Proteintech anti oga antibody
Figure 4. High O-GlcNAcylation increases Elk1 to <t>induce</t> <t>3βHSD1</t> expression and enzyme activity. (A) Protein expression of ELK1 in LNCaP and C42 cells treat- ed with CAF–conditioned medium (CAF-CM) (left) and increasing concentrations of glucosamine (right) for 48 hours. (B) ELK1 protein expression in LNCaP cells expressing control or OGT shRNA (left) and sgRNA (right) treated with CAF-CM or 10 mM glucosamine for 48 hours. (C) Pearson correlation analysis of ELK1 and <t>OGA</t> mRNA expression in prostate cancer (GSE21032). (D) Left: Gene expression of HSD3B1 in control (sgControl) or ELK1-KO (sgELK1) LNCaP cells treated with CAF-CM or 10 mM glucosamine for 48 hours. Right: Luciferase assay of LNCaP control and ELK1-KO cells cotransfected with an HSD3B1 promoter-fire- fly luciferase and Renilla luciferase plasmid constructs, which were treated with CAF-CM or 10 mM glucosamine 48 hours. (E) ChIP assay of Elk1. C4-2 cells were treated with 10 mM glucosamine for 24 hours. (F) Protein expression of ELK1 and 3βHSD1 in sgControl and ELK1-KO LNCaP cells treated with CAF-CM or glucosamine. (G) HPLC analysis of steroids in media of LNCaP cells expressing sgControl and ELK1 KO (left) or siRNA and shRNA knockdown of ELK1 (right). Cells were treated with CAF-CM or glucosamine for 48 hours, followed by [3H]-DHEA (100 nM) for 48 hours. (H) Gene expression of PSA, TMPRSS2, and FKBP5 in sgControl and ELK1-KO LNCaP cells treated with 10 nM DHEA along with CAF-CM or glucosamine for 48 hours. (I) Pearson correlation analysis of ELK1 and HSD3B1 mRNA expression in prostate cancer (GSE21032). Significance was calculated using 2-tailed t tests. *P < 0.05, **P < 0.01.
Anti Oga Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag  (Addgene inc)
85
Addgene inc flag
Figure 4. High O-GlcNAcylation increases Elk1 to <t>induce</t> <t>3βHSD1</t> expression and enzyme activity. (A) Protein expression of ELK1 in LNCaP and C42 cells treat- ed with CAF–conditioned medium (CAF-CM) (left) and increasing concentrations of glucosamine (right) for 48 hours. (B) ELK1 protein expression in LNCaP cells expressing control or OGT shRNA (left) and sgRNA (right) treated with CAF-CM or 10 mM glucosamine for 48 hours. (C) Pearson correlation analysis of ELK1 and <t>OGA</t> mRNA expression in prostate cancer (GSE21032). (D) Left: Gene expression of HSD3B1 in control (sgControl) or ELK1-KO (sgELK1) LNCaP cells treated with CAF-CM or 10 mM glucosamine for 48 hours. Right: Luciferase assay of LNCaP control and ELK1-KO cells cotransfected with an HSD3B1 promoter-fire- fly luciferase and Renilla luciferase plasmid constructs, which were treated with CAF-CM or 10 mM glucosamine 48 hours. (E) ChIP assay of Elk1. C4-2 cells were treated with 10 mM glucosamine for 24 hours. (F) Protein expression of ELK1 and 3βHSD1 in sgControl and ELK1-KO LNCaP cells treated with CAF-CM or glucosamine. (G) HPLC analysis of steroids in media of LNCaP cells expressing sgControl and ELK1 KO (left) or siRNA and shRNA knockdown of ELK1 (right). Cells were treated with CAF-CM or glucosamine for 48 hours, followed by [3H]-DHEA (100 nM) for 48 hours. (H) Gene expression of PSA, TMPRSS2, and FKBP5 in sgControl and ELK1-KO LNCaP cells treated with 10 nM DHEA along with CAF-CM or glucosamine for 48 hours. (I) Pearson correlation analysis of ELK1 and HSD3B1 mRNA expression in prostate cancer (GSE21032). Significance was calculated using 2-tailed t tests. *P < 0.05, **P < 0.01.
Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag - by Bioz Stars, 2026-02
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glass lens 27420  (Edmund Optics)
90
Edmund Optics glass lens 27420
Figure 4. High O-GlcNAcylation increases Elk1 to <t>induce</t> <t>3βHSD1</t> expression and enzyme activity. (A) Protein expression of ELK1 in LNCaP and C42 cells treat- ed with CAF–conditioned medium (CAF-CM) (left) and increasing concentrations of glucosamine (right) for 48 hours. (B) ELK1 protein expression in LNCaP cells expressing control or OGT shRNA (left) and sgRNA (right) treated with CAF-CM or 10 mM glucosamine for 48 hours. (C) Pearson correlation analysis of ELK1 and <t>OGA</t> mRNA expression in prostate cancer (GSE21032). (D) Left: Gene expression of HSD3B1 in control (sgControl) or ELK1-KO (sgELK1) LNCaP cells treated with CAF-CM or 10 mM glucosamine for 48 hours. Right: Luciferase assay of LNCaP control and ELK1-KO cells cotransfected with an HSD3B1 promoter-fire- fly luciferase and Renilla luciferase plasmid constructs, which were treated with CAF-CM or 10 mM glucosamine 48 hours. (E) ChIP assay of Elk1. C4-2 cells were treated with 10 mM glucosamine for 24 hours. (F) Protein expression of ELK1 and 3βHSD1 in sgControl and ELK1-KO LNCaP cells treated with CAF-CM or glucosamine. (G) HPLC analysis of steroids in media of LNCaP cells expressing sgControl and ELK1 KO (left) or siRNA and shRNA knockdown of ELK1 (right). Cells were treated with CAF-CM or glucosamine for 48 hours, followed by [3H]-DHEA (100 nM) for 48 hours. (H) Gene expression of PSA, TMPRSS2, and FKBP5 in sgControl and ELK1-KO LNCaP cells treated with 10 nM DHEA along with CAF-CM or glucosamine for 48 hours. (I) Pearson correlation analysis of ELK1 and HSD3B1 mRNA expression in prostate cancer (GSE21032). Significance was calculated using 2-tailed t tests. *P < 0.05, **P < 0.01.
Glass Lens 27420, supplied by Edmund Optics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. High O-GlcNAcylation increases Elk1 to induce 3βHSD1 expression and enzyme activity. (A) Protein expression of ELK1 in LNCaP and C42 cells treat- ed with CAF–conditioned medium (CAF-CM) (left) and increasing concentrations of glucosamine (right) for 48 hours. (B) ELK1 protein expression in LNCaP cells expressing control or OGT shRNA (left) and sgRNA (right) treated with CAF-CM or 10 mM glucosamine for 48 hours. (C) Pearson correlation analysis of ELK1 and OGA mRNA expression in prostate cancer (GSE21032). (D) Left: Gene expression of HSD3B1 in control (sgControl) or ELK1-KO (sgELK1) LNCaP cells treated with CAF-CM or 10 mM glucosamine for 48 hours. Right: Luciferase assay of LNCaP control and ELK1-KO cells cotransfected with an HSD3B1 promoter-fire- fly luciferase and Renilla luciferase plasmid constructs, which were treated with CAF-CM or 10 mM glucosamine 48 hours. (E) ChIP assay of Elk1. C4-2 cells were treated with 10 mM glucosamine for 24 hours. (F) Protein expression of ELK1 and 3βHSD1 in sgControl and ELK1-KO LNCaP cells treated with CAF-CM or glucosamine. (G) HPLC analysis of steroids in media of LNCaP cells expressing sgControl and ELK1 KO (left) or siRNA and shRNA knockdown of ELK1 (right). Cells were treated with CAF-CM or glucosamine for 48 hours, followed by [3H]-DHEA (100 nM) for 48 hours. (H) Gene expression of PSA, TMPRSS2, and FKBP5 in sgControl and ELK1-KO LNCaP cells treated with 10 nM DHEA along with CAF-CM or glucosamine for 48 hours. (I) Pearson correlation analysis of ELK1 and HSD3B1 mRNA expression in prostate cancer (GSE21032). Significance was calculated using 2-tailed t tests. *P < 0.05, **P < 0.01.

Journal: Journal of Clinical Investigation

Article Title: Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation

doi: 10.1172/jci161913

Figure Lengend Snippet: Figure 4. High O-GlcNAcylation increases Elk1 to induce 3βHSD1 expression and enzyme activity. (A) Protein expression of ELK1 in LNCaP and C42 cells treat- ed with CAF–conditioned medium (CAF-CM) (left) and increasing concentrations of glucosamine (right) for 48 hours. (B) ELK1 protein expression in LNCaP cells expressing control or OGT shRNA (left) and sgRNA (right) treated with CAF-CM or 10 mM glucosamine for 48 hours. (C) Pearson correlation analysis of ELK1 and OGA mRNA expression in prostate cancer (GSE21032). (D) Left: Gene expression of HSD3B1 in control (sgControl) or ELK1-KO (sgELK1) LNCaP cells treated with CAF-CM or 10 mM glucosamine for 48 hours. Right: Luciferase assay of LNCaP control and ELK1-KO cells cotransfected with an HSD3B1 promoter-fire- fly luciferase and Renilla luciferase plasmid constructs, which were treated with CAF-CM or 10 mM glucosamine 48 hours. (E) ChIP assay of Elk1. C4-2 cells were treated with 10 mM glucosamine for 24 hours. (F) Protein expression of ELK1 and 3βHSD1 in sgControl and ELK1-KO LNCaP cells treated with CAF-CM or glucosamine. (G) HPLC analysis of steroids in media of LNCaP cells expressing sgControl and ELK1 KO (left) or siRNA and shRNA knockdown of ELK1 (right). Cells were treated with CAF-CM or glucosamine for 48 hours, followed by [3H]-DHEA (100 nM) for 48 hours. (H) Gene expression of PSA, TMPRSS2, and FKBP5 in sgControl and ELK1-KO LNCaP cells treated with 10 nM DHEA along with CAF-CM or glucosamine for 48 hours. (I) Pearson correlation analysis of ELK1 and HSD3B1 mRNA expression in prostate cancer (GSE21032). Significance was calculated using 2-tailed t tests. *P < 0.05, **P < 0.01.

Article Snippet: Primary antibodies used were anti-3βHSD1 antibody (Abcam, 55268; 1:1,000), anti-OGT antibody (Proteintech, 11576; 1:4,000), anti-OGA antibody (Proteintech, 14711; 1:4,000), anti-Elk1 antibody (Proteintech, 27420; 1:4,000), and anti-O-GlcNac antibody (Invitrogen, MA1-072; 1:2,000).

Techniques: Expressing, Activity Assay, Control, shRNA, Gene Expression, Luciferase, Plasmid Preparation, Construct, Knockdown