26495 Search Results


atg12  (Novus Biologicals)
90
Novus Biologicals atg12
CNS tumor cells with BRAFV600E have high rates of induced autophagy and sensitivity to autophagy inhibition. (A) Cells with mCh-GFP-LC3 were exposed to either standard media or starvation EBSS media for 4 hours and analyzed for the change in ratio of mCh to GFP signal as a measure of autophagic flux. * P < 0.05. (B) Cells expressing control, ATG5, or <t>ATG12</t> shRNAs were plated in standard media and allowed to grow for 72 hours before analysis by MTS assay. * P < 0.05. (C) Cells were plated as in (B) and were monitored every 4 hours by light microscopy using real time in vitro imaging. Quantitative analysis of confluence was performed using the IncuCyte system. Data shown are mean ± SEM of a representative experiment. (D) Representative immunoblot demonstrating knockdown of baseline Atg5 and Atg12 protein levels after 72 hours of RNAi for experiments shown in (B–C). (E) WT BT16 and BRAFV600E 794, AM38 and NMC-G1 mutant cells were treated with increasing doses of CQ for 48 hours and cell viability was evaluated by LDH release and MTS assay. (F) Cells were treated as in (E) and evaluated for the percentage of PI positive cells at 48 hours.
Atg12, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atg12/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
atg12 - by Bioz Stars, 2026-06
90/100 stars
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alteromonas macleodii jcm 20772t  (DSMZ)
92
DSMZ alteromonas macleodii jcm 20772t
CNS tumor cells with BRAFV600E have high rates of induced autophagy and sensitivity to autophagy inhibition. (A) Cells with mCh-GFP-LC3 were exposed to either standard media or starvation EBSS media for 4 hours and analyzed for the change in ratio of mCh to GFP signal as a measure of autophagic flux. * P < 0.05. (B) Cells expressing control, ATG5, or <t>ATG12</t> shRNAs were plated in standard media and allowed to grow for 72 hours before analysis by MTS assay. * P < 0.05. (C) Cells were plated as in (B) and were monitored every 4 hours by light microscopy using real time in vitro imaging. Quantitative analysis of confluence was performed using the IncuCyte system. Data shown are mean ± SEM of a representative experiment. (D) Representative immunoblot demonstrating knockdown of baseline Atg5 and Atg12 protein levels after 72 hours of RNAi for experiments shown in (B–C). (E) WT BT16 and BRAFV600E 794, AM38 and NMC-G1 mutant cells were treated with increasing doses of CQ for 48 hours and cell viability was evaluated by LDH release and MTS assay. (F) Cells were treated as in (E) and evaluated for the percentage of PI positive cells at 48 hours.
Alteromonas Macleodii Jcm 20772t, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alteromonas macleodii jcm 20772t/product/DSMZ
Average 92 stars, based on 1 article reviews
alteromonas macleodii jcm 20772t - by Bioz Stars, 2026-06
92/100 stars
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an upper punch and a lower punch based on the punch tip faces described above (p-266644-a, p-26493-a, p-26494-a, p-26495-a)  (MANESTY MACHINES LIMITED)
90
MANESTY MACHINES LIMITED an upper punch and a lower punch based on the punch tip faces described above (p-266644-a, p-26493-a, p-26494-a, p-26495-a)
CNS tumor cells with BRAFV600E have high rates of induced autophagy and sensitivity to autophagy inhibition. (A) Cells with mCh-GFP-LC3 were exposed to either standard media or starvation EBSS media for 4 hours and analyzed for the change in ratio of mCh to GFP signal as a measure of autophagic flux. * P < 0.05. (B) Cells expressing control, ATG5, or <t>ATG12</t> shRNAs were plated in standard media and allowed to grow for 72 hours before analysis by MTS assay. * P < 0.05. (C) Cells were plated as in (B) and were monitored every 4 hours by light microscopy using real time in vitro imaging. Quantitative analysis of confluence was performed using the IncuCyte system. Data shown are mean ± SEM of a representative experiment. (D) Representative immunoblot demonstrating knockdown of baseline Atg5 and Atg12 protein levels after 72 hours of RNAi for experiments shown in (B–C). (E) WT BT16 and BRAFV600E 794, AM38 and NMC-G1 mutant cells were treated with increasing doses of CQ for 48 hours and cell viability was evaluated by LDH release and MTS assay. (F) Cells were treated as in (E) and evaluated for the percentage of PI positive cells at 48 hours.
An Upper Punch And A Lower Punch Based On The Punch Tip Faces Described Above (P 266644 A, P 26493 A, P 26494 A, P 26495 A), supplied by MANESTY MACHINES LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/an upper punch and a lower punch based on the punch tip faces described above (p-266644-a, p-26493-a, p-26494-a, p-26495-a)/product/MANESTY MACHINES LIMITED
Average 90 stars, based on 1 article reviews
an upper punch and a lower punch based on the punch tip faces described above (p-266644-a, p-26493-a, p-26494-a, p-26495-a) - by Bioz Stars, 2026-06
90/100 stars
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pjc153.5-grh-1 (plasmid #26495)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


CNS tumor cells with BRAFV600E have high rates of induced autophagy and sensitivity to autophagy inhibition. (A) Cells with mCh-GFP-LC3 were exposed to either standard media or starvation EBSS media for 4 hours and analyzed for the change in ratio of mCh to GFP signal as a measure of autophagic flux. * P < 0.05. (B) Cells expressing control, ATG5, or ATG12 shRNAs were plated in standard media and allowed to grow for 72 hours before analysis by MTS assay. * P < 0.05. (C) Cells were plated as in (B) and were monitored every 4 hours by light microscopy using real time in vitro imaging. Quantitative analysis of confluence was performed using the IncuCyte system. Data shown are mean ± SEM of a representative experiment. (D) Representative immunoblot demonstrating knockdown of baseline Atg5 and Atg12 protein levels after 72 hours of RNAi for experiments shown in (B–C). (E) WT BT16 and BRAFV600E 794, AM38 and NMC-G1 mutant cells were treated with increasing doses of CQ for 48 hours and cell viability was evaluated by LDH release and MTS assay. (F) Cells were treated as in (E) and evaluated for the percentage of PI positive cells at 48 hours.

Journal: Cancer discovery

Article Title: Autophagy Inhibition Improves Chemosensitivity in BRAF V600E Brain Tumors

doi: 10.1158/2159-8290.CD-14-0049

Figure Lengend Snippet: CNS tumor cells with BRAFV600E have high rates of induced autophagy and sensitivity to autophagy inhibition. (A) Cells with mCh-GFP-LC3 were exposed to either standard media or starvation EBSS media for 4 hours and analyzed for the change in ratio of mCh to GFP signal as a measure of autophagic flux. * P < 0.05. (B) Cells expressing control, ATG5, or ATG12 shRNAs were plated in standard media and allowed to grow for 72 hours before analysis by MTS assay. * P < 0.05. (C) Cells were plated as in (B) and were monitored every 4 hours by light microscopy using real time in vitro imaging. Quantitative analysis of confluence was performed using the IncuCyte system. Data shown are mean ± SEM of a representative experiment. (D) Representative immunoblot demonstrating knockdown of baseline Atg5 and Atg12 protein levels after 72 hours of RNAi for experiments shown in (B–C). (E) WT BT16 and BRAFV600E 794, AM38 and NMC-G1 mutant cells were treated with increasing doses of CQ for 48 hours and cell viability was evaluated by LDH release and MTS assay. (F) Cells were treated as in (E) and evaluated for the percentage of PI positive cells at 48 hours.

Article Snippet: Primary antibodies used were: ATG5, ATG12 and LC3 (Novus Biologicals, Littleton, CO).

Techniques: Inhibition, Expressing, MTS Assay, Light Microscopy, In Vitro, Imaging, Western Blot, Mutagenesis