Journal: Cancer Research

Article Title: Loss of Histone Deacetylase 4 Causes Segregation Defects during Mitosis of p53-Deficient Human Tumor Cells

doi: 10.1158/0008-5472.can-08-2796

Figure Lengend Snippet: Figure 2. Loss of HDAC4 is well tolerated in normal cells. A, expression levels of HDAC4 in human normal tissues show that HDAC4 has a much more tissue-restricted expression, specifically, in muscle and testis, as compared with the ubiquitously expressed HDAC1 and HDAC3. B, parallel transfection of HCT-116 cells and NHDF with HDAC4.13 siRNA led to a cell death phenotype at 72 h posttransfection only in tumor cells, as shown by cell detachment and membrane blebbing (a), despite similar knockdown efficiencies measured at 24 h (b). C, human myelopoietic progenitors transfected with HDAC4 siRNAs; despite down-regulation of HDAC4 expression, no decrease in clonogenicity was observed. GL2 siRNA served as a negative control, and Eg5 siRNA as a positive control.

Article Snippet: HDAC4 and phosphorylated H3 antibodies were from Cell Signaling, a-tubulin antibody was from Sigma, and Eg5 and PARP antibodies were from Novus Biologicals.

Techniques: Expressing, Transfection, Membrane, Knockdown, Negative Control, Positive Control

Journal: bioRxiv

Article Title: Ecology and pathogenicity for honey bee brood of recently described Paenibacillus melissococcoides and comparison with Paenibacillus dendritiformis, Paenibacillus thiaminolyticus

doi: 10.1101/2025.02.06.636850

Figure Lengend Snippet: Survival profile of in vitro reared larvae inoculated at first instar stage with three isolates of Paenibacillus melissococcoides, and one isolate each of Paenibacillus dendritiformis, Paenibacillus thiaminolyticus and Melissococcus plutonius , compared with non-inoculated control larvae. Sample size is indicated in the legend. The asterisks represent significant differences between the groups’ survival curves (log-rank tests, p < 0.05, Table S2).

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 , and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in .

Techniques: In Vitro, Control

Journal: bioRxiv

Article Title: Ecology and pathogenicity for honey bee brood of recently described Paenibacillus melissococcoides and comparison with Paenibacillus dendritiformis, Paenibacillus thiaminolyticus

doi: 10.1101/2025.02.06.636850

Figure Lengend Snippet: Number of colony forming units in suspensions of Paenibacillus melissococcoides , Paenibacillus dendritiformis and Paenibacillus thiaminolyticus immediately before, 1.5 and 4h after mixing with the diet. The number of spores in the suspensions was calculated from the percentage of spores evaluated by microscopy before mixing with the diet.

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 , and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in .

Techniques: Microscopy

Journal: bioRxiv

Article Title: Ecology and pathogenicity for honey bee brood of recently described Paenibacillus melissococcoides and comparison with Paenibacillus dendritiformis, Paenibacillus thiaminolyticus

doi: 10.1101/2025.02.06.636850

Figure Lengend Snippet: Location of apiaries screened for Paenibacillus melissococcoides in the period 2005-2021 in Switzerland. Number of apiaries analyzed per year and symptom status is indicated next to the corresponding symbol in the legend: circles correspond to screened apiaries with EFB-symptomatic colonies, triangles to screened apiaries with asymptomatic colonies and squares to idiopathic cases. Colors represent the different years. In many occasions, apiaries screened were so close to each other that their symbols overlap completely.

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 , and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in .

Techniques:

Journal: bioRxiv

Article Title: Ecology and pathogenicity for honey bee brood of recently described Paenibacillus melissococcoides and comparison with Paenibacillus dendritiformis, Paenibacillus thiaminolyticus

doi: 10.1101/2025.02.06.636850

Figure Lengend Snippet: Survival profiles of in vitro reared larvae inoculated with Paenibacillus melissococcoides 3.2 and of non-inoculated control larvae. A. Dose dependence: one-day old larvae inoculated the first day of rearing with different doses of P. melissococcoides (in CFUs) and controls. B. Age dependence: one-, three- and five-day old larvae inoculated with 10 5 P. melissococcoides bacteria (arrows), respectively, and controls. Sample size is indicated in the legend. Different letters to the right of the curves indicate significant differences in the survival of the tested dose and age groups (pairwise log-rank tests, Bonferroni-Holm corrected, p<0.05).

Article Snippet: Three P. melissococcoides isolates, 1.2, 2.1 and 3.2 , and two closely related Paenibacillus species, P. dendritiformis LMG 21716 (type strain, provided by BCCM LMG) and P. thiaminolyticus DSM 7262 (type strain, provided by DSMZ GmbH) were cultivated on basal medium Petri dishes for four days at 36°C under oxic conditions as recommended in .

Techniques: In Vitro, Control, Bacteria