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Image Search Results
Journal: Translational Psychiatry
Article Title: Acute stress induces severe neural inflammation and overactivation of glucocorticoid signaling in interleukin-18-deficient mice
doi: 10.1038/s41398-022-02175-7
Figure Lengend Snippet: A The heatmap (left) and volcano map (right) generated from RNA sequencing are shown. ( C ; stressed Il18 +/+ mice, KO; stressed Il18 −/− mice). B The reverse transcription quantitative polymerase chain reaction results are shown. The molecules were extracted from RNA sequencing as shown in Table . C The protein expression of gamma-aminobutyric acid type A receptor subunit rho1 (GABRR1), heat shock transcription factor 5 (HSF5), and transthyretin (TTR) is shown. D The mRNA, protein, and serum levels of TTR/Ttr are shown. * p < 0.05, ** p < 0.01, *** p < 0.001. ( A ; n = 3, B – D ; n = 6, enzyme-linked immunosorbent assay n = 12 per group).
Article Snippet: The primary antibodies were as follows: monoclonal rabbit anti-β-actin (cat. no. #4967; Cell Signaling Technology, Inc. (CST), Danvers, MA, USA) or anti-GAPDH (cat. no. #3683, CST) as a loading control, rabbit anti-heat shock transcription factor 5 (HSF5) polyclonal antibody (cat. No. MBS2520556, MyBioSource, San Diego, CA, USA),
Techniques: Generated, RNA Sequencing, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay
Journal: Translational Psychiatry
Article Title: Acute stress induces severe neural inflammation and overactivation of glucocorticoid signaling in interleukin-18-deficient mice
doi: 10.1038/s41398-022-02175-7
Figure Lengend Snippet: Nine genes with a false discovery rate <0.30 were extracted from the RNA sequencing results of Il18 -deficient mice and assigned GenBank accession numbers.
Article Snippet: The primary antibodies were as follows: monoclonal rabbit anti-β-actin (cat. no. #4967; Cell Signaling Technology, Inc. (CST), Danvers, MA, USA) or anti-GAPDH (cat. no. #3683, CST) as a loading control, rabbit anti-heat shock transcription factor 5 (HSF5) polyclonal antibody (cat. No. MBS2520556, MyBioSource, San Diego, CA, USA),
Techniques: RNA Sequencing