Journal: Biosensors

Article Title: Development of a Novel Phagomagnetic-Assisted Isothermal DNA Amplification System for Endpoint Electrochemical Detection of Listeria monocytogenes

doi: 10.3390/bios13040464

Figure Lengend Snippet: Bacterial strains used for LAMP evaluation and PCR validation.

Article Snippet: Campylobacter spp. , C. jejuni , DSMZ 4688 , 1 , - , -.

Techniques: Biomarker Discovery

Journal: Cancer science

Article Title: Hypoxia Inhibitor Improves Iodine Uptake Disorder in Thyroid Cancer Through the hsa_circ_0023990/miR-448/DNMT1/NIS Axis.

doi: 10.1111/cas.70102

Figure Lengend Snippet: FIGURE 2 | hsa_circ_0023990 regulates NIS expression through affecting its methylation level. (A, B) The DNMT1 and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.

Article Snippet: These membranes were subsequently incubated with primary antibodies, such as anti- DNMT1 (24206- 1- AP), anti- NIS (24324- 1- AP), and GAPDH (60004- 1- lg; Proteintech) at the recommended concentration overnight at 4°C.

Techniques: Expressing, Methylation, Western Blot, MSP Assay, Transfection, Over Expression, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction

Journal: Cancer science

Article Title: Hypoxia Inhibitor Improves Iodine Uptake Disorder in Thyroid Cancer Through the hsa_circ_0023990/miR-448/DNMT1/NIS Axis.

doi: 10.1111/cas.70102

Figure Lengend Snippet: FIGURE 6 | Hypoxia inhibitors increase iodine uptake in tumor tissue through the has_circ_0023990/DNMT1/NIS signaling axis to inhibit tu- mor growth. (A) H&E staining. Scale bar = 50 μm. (B, C) The cell proliferation in tumor tissues was detected by Ki-67 immunohistochemistry. “IOD/ Area” indicating the mean optical density of Ki-67 expression. Scale bar = 50 μm. (D, E) The cell apoptosis in tumor tissues was detected by TUNEL staining. Scale bar = 50 μm. (F) The hsa_circ_0023990 expression in tumor tissues was detected by RT-PCR. (G–I) The DNMT1 and NIS protein ex- pressions in tumor tissues were detected by western blot. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: These membranes were subsequently incubated with primary antibodies, such as anti- DNMT1 (24206- 1- AP), anti- NIS (24324- 1- AP), and GAPDH (60004- 1- lg; Proteintech) at the recommended concentration overnight at 4°C.

Techniques: Staining, Immunohistochemistry, Expressing, TUNEL Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot