Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Clinicopathological Characteristics of the Study Population

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The levels of serum AFP, GPC3, GP73 and DCP in each subgroup. ( A ) AFP. ( B ) GPC3. ( C ) GP73. ( D ) DCP. ( E ) Representative image of each subgroup. * P <0.05, ** P <0.01. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin; ns, no significance; int, intensity.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Virus, Infection

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Diagnosis of HCC (Including All HCC Patients)

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: Assessment of the diagnostic value of serum AFP, GPC3, GP73 and DCP in differentiating HBV-related HCC from controls. ( A ) All HCC vs LC, CHB, HC. ( B ) All HCC vs LC, CHB. ( C ) All HCC v s LC. ( D ) Very early and early stage HCC vs LC, CHB, HC. ( E ) Very early and early stage HCC vs LC, CHB. ( F ) Very early and early stage HCC vs LC. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Diagnostic Assay, Virus, Infection

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Early Diagnosis of HCC (Including the Very Early HCC)

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The Value of Serum AFP, GPC3, GP73 and DCP in the Very Early Diagnosis of HCC

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques:

Journal: OncoTargets and therapy

Article Title: Dynamic Changes in Serum Markers and Their Utility in the Early Diagnosis of All Stages of Hepatitis B-Associated Hepatocellular Carcinoma

doi: 10.2147/OTT.S229835

Figure Lengend Snippet: The value of serum AFP, GPC3, GP73 and DCP in the very early diagnosis of HBV-related HCC. ( A ) Very early stage HCC vs LC, CHB, HC. ( B ) Very early stage vs LC, CHB. ( C ) Very early stage vs LC. Abbreviations: HCC, hepatocellular carcinoma; LC, liver cirrhosis; CHB, chronic hepatitis B virus infection; HC, healthy controls; AFP, α-fetoprotein; GPC3, glypican 3; GP73, golgi protein 73; DCP, des-γ-carboxy prothrombin.

Article Snippet: The following antibodies were spotted on aldehyde-coated microscope slides (Shanghai Baiao, China) by a GeSiM Nano-Plotter TM Micropipetting System (Radeberg, Germany): mouse monoclonal AFP antibody (1.25 mg/mL, Frpon Botech Inc, Shenzhen, China), mouse monoclonal GPC3 antibody (1 mg/mL, R&D Bio-techne, Minneapolis, USA), mouse monoclonal Golgi membrane protein 1 (GOLM1) antibody (0.5 mg/mL, Novus, Centennial, USA) and mouse monoclonal prothrombin factor II antibody (2.5mg/mL, Fujirebio Inc, Tokyo, Japan).

Techniques: Virus, Infection

Journal: Heliyon

Article Title: CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer

doi: 10.1016/j.heliyon.2023.e19220

Figure Lengend Snippet: CD200 and CTSK expression in cervical cancer tissues. (A) The GEPIA online website was used to analyze CD200 and CTSK expression in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) tissues and normal tissues. (B) The correlation of CD200 and CTSK in the CESC. CD200 (C) and CTSK (D) expression in tumor tissues and para-cancerous tissues was measured using immunohistochemistry. *P < 0.05, **P < 0.01. Representative images of CD200 and CTSK expression in tissues are provided in Supplemental and .

Article Snippet: Diluted primary CD200 rabbit antibody (#23451S, 1:200, Cell Signaling Technology, Shanghai, China), CD200R rabbit-antibody (1:400, #DF4715, Affinity, China), Ki-67 rabbit-antibody (1:400, #9027, Cell Signaling Technology) and CTSK rabbit antibody (1:400, #DF6614, Affinity, China) were incubated with the section at 4 °C for overnight.

Techniques: Expressing, Immunohistochemistry

Journal: Heliyon

Article Title: CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer

doi: 10.1016/j.heliyon.2023.e19220

Figure Lengend Snippet: Effects of CD200 on the viability and invasion of HeLa and Siha cells. HeLa and Siha cells were stably transfected with 2 μg of control plasmid or PUNO1-hCD200. (A) Cell viabilities were analyzed by CCK-8. (B) Cell invasion was measured by Transwell assay. Scale = 50 μm. The results of CD200 transfection in HeLa and SiHa cells are provided in Supplemental and . NS: no significance.

Article Snippet: Diluted primary CD200 rabbit antibody (#23451S, 1:200, Cell Signaling Technology, Shanghai, China), CD200R rabbit-antibody (1:400, #DF4715, Affinity, China), Ki-67 rabbit-antibody (1:400, #9027, Cell Signaling Technology) and CTSK rabbit antibody (1:400, #DF6614, Affinity, China) were incubated with the section at 4 °C for overnight.

Techniques: Stable Transfection, Transfection, Control, Plasmid Preparation, CCK-8 Assay, Transwell Assay

Journal: Heliyon

Article Title: CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer

doi: 10.1016/j.heliyon.2023.e19220

Figure Lengend Snippet: CD200-CD200R regulated the CTSK-mediated p65 NF-κB pathway in cervical cancer cells. Cancer cells were cocultured without or with M2 macrophage-like THP-1 cells. Expression levels of CTSK and p-p65 NF-κB/p65 NF-κB protein in HeLa (A) and Siha (B) were measured by Western blotting. The full length of blots was provided in Supplemental materials. *P < 0.05, **P < 0.01.

Article Snippet: Diluted primary CD200 rabbit antibody (#23451S, 1:200, Cell Signaling Technology, Shanghai, China), CD200R rabbit-antibody (1:400, #DF4715, Affinity, China), Ki-67 rabbit-antibody (1:400, #9027, Cell Signaling Technology) and CTSK rabbit antibody (1:400, #DF6614, Affinity, China) were incubated with the section at 4 °C for overnight.

Techniques: Expressing, Western Blot

Journal: Heliyon

Article Title: CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer

doi: 10.1016/j.heliyon.2023.e19220

Figure Lengend Snippet: CD200-CD200R is involved in the response to cisplatin or paclitaxel in cervical cancer. (A) Schematic diagram shows a coculture system of M2 macrophage-like THP-1 cells and cancer cells. Cell viabilities of HeLa (B) and Siha (C) cells were analyzed by CCK-8. Cell invasion of HeLa (D) and Siha (E) cells was analyzed by Transwell assay. The images of Transwell assay are provided in Supplemental . *P < 0.05, **P < 0.01.

Article Snippet: Diluted primary CD200 rabbit antibody (#23451S, 1:200, Cell Signaling Technology, Shanghai, China), CD200R rabbit-antibody (1:400, #DF4715, Affinity, China), Ki-67 rabbit-antibody (1:400, #9027, Cell Signaling Technology) and CTSK rabbit antibody (1:400, #DF6614, Affinity, China) were incubated with the section at 4 °C for overnight.

Techniques: CCK-8 Assay, Transwell Assay

Journal: Heliyon

Article Title: CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer

doi: 10.1016/j.heliyon.2023.e19220

Figure Lengend Snippet: CD200-CD200R affects the chemosensitivity of cervical cancer cells through regulating the CTSK-mediated p65 NF-κB pathway. Cancer cells were treated with 0.1 μg/mL rCTSK as a control to compare the effects of C200-CD200R on resistance to cisplatin or paclitaxel. Cell viabilities of HeLa (A) and Siha (B) were analyzed by CCK-8. Expression of p-p65 NF-κB/p65 NF-κB protein in HeLa (C) and Siha (D) was analyzed by Western blotting. The full length of blots was provided in Supplemental materials. **P < 0.01.

Article Snippet: Diluted primary CD200 rabbit antibody (#23451S, 1:200, Cell Signaling Technology, Shanghai, China), CD200R rabbit-antibody (1:400, #DF4715, Affinity, China), Ki-67 rabbit-antibody (1:400, #9027, Cell Signaling Technology) and CTSK rabbit antibody (1:400, #DF6614, Affinity, China) were incubated with the section at 4 °C for overnight.

Techniques: Control, CCK-8 Assay, Expressing, Western Blot

Journal: Heliyon

Article Title: CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer

doi: 10.1016/j.heliyon.2023.e19220

Figure Lengend Snippet: CD200-C200R affects the paclitaxel sensitivity in mouse xenografts through regulating CTSK. Stably transfected plasmid or CD200 HeLa cells were used to establish xenografts. After injection for 7 days, 3.6 mg/kg MK-0822 (CTSK inhibitor) was orally treated every week, and 5 mg/kg paclitaxel was administrated by tail vein twice a week. (A) Tumor size was measured every week. (B) Images of xenografts and tumor weight. (C) Positive expression of Ki-67 in tumor tissues of each group. Scale = 50 μm. NS: no significance. **P < 0.01.

Article Snippet: Diluted primary CD200 rabbit antibody (#23451S, 1:200, Cell Signaling Technology, Shanghai, China), CD200R rabbit-antibody (1:400, #DF4715, Affinity, China), Ki-67 rabbit-antibody (1:400, #9027, Cell Signaling Technology) and CTSK rabbit antibody (1:400, #DF6614, Affinity, China) were incubated with the section at 4 °C for overnight.

Techniques: Stable Transfection, Transfection, Plasmid Preparation, Injection, Expressing

Journal: Heliyon

Article Title: CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer

doi: 10.1016/j.heliyon.2023.e19220

Figure Lengend Snippet: A CD200-CD200R mediated feedback loop between cancer cells and M2-tumor-associated macrophages (TAMs) in cervical cancer.

Article Snippet: Diluted primary CD200 rabbit antibody (#23451S, 1:200, Cell Signaling Technology, Shanghai, China), CD200R rabbit-antibody (1:400, #DF4715, Affinity, China), Ki-67 rabbit-antibody (1:400, #9027, Cell Signaling Technology) and CTSK rabbit antibody (1:400, #DF6614, Affinity, China) were incubated with the section at 4 °C for overnight.

Techniques: