Journal: Persoonia : Molecular Phylogeny and Evolution of Fungi

Article Title: Generic hyper-diversity in Stachybotriaceae

doi: 10.3767/003158516X691582

Figure Lengend Snippet: Details of strains included in phylogenetic analyses. GenBank accession numbers in italics were newly generated in this study.

Article Snippet: Mem. oenanthes , ATCC 22844 = IMI 016185 , Oenanthe crocata , E.A. Ellis & M.B. Ellis , Channel Islands , – , AF081473 , – , – , – , –.

Techniques: Generated, Gypsum, Insulation

Journal: Cells

Article Title: Apoptosis, Induced by Human α-Synuclein in Yeast, Can Occur Independent of Functional Mitochondria

doi: 10.3390/cells9102203

Figure Lengend Snippet: ( A , B ) Microscopic images (×400) of staining of ρ − petite transformants, that bear chromosomally integrated α-syn-containing plasmids, with the dye DiOC6(3) that detects MMP in live cells. The images are representative images (×400) of cells. ( C , D ) Quantification of relative MMP of yeast cells expressing 2 and 3-copies of α-syn from MET25 p and 1–3-copies of α-syn from GAL1 p, using the JC-10 dye ( p < 0.1). ( E , F ) Microscopic images (×400) of nuclear DNA fragmentation, as observed using the TUNEL assay, in yeast cells expressing 2 and 3-copies of α-syn from MET25 p and 1–3-copies of α-syn from GAL1 p. The images are representative images of cells. The data in figures ( C , D ) represent mean ± S.D. of three independent experiments ( p < 0.1; two-tailed t -test). The left-hand side pictures in ( A , B , E , F ) show phase-contrast microscopy pictures (×400) of yeast cells. Post Hoc Newman-Keuls test, after a significant one-way ANOVA test, indicated a significant difference p < 0.001 between strains expressing 2 and 3-copies of α-syn from MET25 p, and between 1 and 2-copies and 3-copies of α-syn from GAL1 p.

Article Snippet: AAT Bioquest (Sunnyvale, CA, USA) TUNEL Apoptosis Assay kit (#22844) was used for the detection of nuclear DNA fragmentation (NDF).

Techniques: Staining, Expressing, TUNEL Assay, Two Tailed Test, Microscopy

Journal: Cells

Article Title: Apoptosis, Induced by Human α-Synuclein in Yeast, Can Occur Independent of Functional Mitochondria

doi: 10.3390/cells9102203

Figure Lengend Snippet: ( A ) Representative pictures of growth of ρ 0 yeast cells harboring 1-copy (1 C ), 2-copies (2 C ), and 3-copies (3 C ) of the human α-syn gene, under the control of either MET25 p, on complete YPD medium agar plates in the presence (YPD + Methionine) or absence (YPD) of methionine. ( B ) Western blot analyses of ρ 0 cells expressing 1-copy (lane 1), 2-copies (lane 2), and 3-copies (lane 3) of α-syn protein. On lanes 1, 2, and 3 were loaded 7.5 µg of total protein obtained after lysis of cells. The upper panel was probed with an antibody that recognizes the HA epitope (Proteintech, 51064-2-AP) and the lower panel with a β-actin antibody (Proteintech, 60008-1-Ig); levels of β-actin were used as loading controls, β-actin being a housekeeping gene. ( C ) Microscopic images (×400) of ρ 0 cells, stained with Phloxine B, after the expression of 1–3 copies of α-syn from the MET25 p. ( D ) Microscopic images (×400) of nuclear DNA fragmentation, as observed using the TUNEL assay, in ρ 0 yeast cells expressing 1–3-copies of α-syn from MET25 p. ( E ) ρ 0 cells, bearing 1–3 copies of MET25 p-driven α-syn expression cassettes, were stained with Hoechst 33,342 (a blue dye that labels DNA of live cells). Post Hoc Newman-Keuls test after a significant one-way ANOVA test, indicated a significant difference between petites expressing 1–3 copies of α-syn.

Article Snippet: AAT Bioquest (Sunnyvale, CA, USA) TUNEL Apoptosis Assay kit (#22844) was used for the detection of nuclear DNA fragmentation (NDF).

Techniques: Control, Western Blot, Expressing, Lysis, Staining, TUNEL Assay

Journal: Cells

Article Title: Apoptosis, Induced by Human α-Synuclein in Yeast, Can Occur Independent of Functional Mitochondria

doi: 10.3390/cells9102203

Figure Lengend Snippet: Analysis of nuclear DNA fragmentation ( A ), an increase of reactive oxygen species (ROS; B ), and loss of mitochondrial membrane potential (MMP; C ), in ρ 0 yeast cells that bear 1 to 3-copies of the human α-syn, gene under the control of the MET25 promoter. ( A ) Levels of nuclear DNA fragmentation, a hallmark of apoptosis, in yeast cells that express 1 to 3-copies of α-syn were assessed using the TUNEL assay. ( B ) Cells were stained with dihydroethidine to detect ROS. ( C ) Cells were stained with the JC-10 dye to quantify MMP loss. The data presented in ( A , B , C ) represent mean ± S.D. of three independent experiments ( p < 0.05).

Article Snippet: AAT Bioquest (Sunnyvale, CA, USA) TUNEL Apoptosis Assay kit (#22844) was used for the detection of nuclear DNA fragmentation (NDF).

Techniques: Membrane, Control, TUNEL Assay, Staining