Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel Engineered Peptides of a Phage Lysin as Effective Antimicrobials against Multidrug-Resistant Acinetobacter baumannii

doi: 10.1128/AAC.02972-15

Figure Lengend Snippet: Peptide derivatives of PlyF307 a

Article Snippet: TABLE 2 A. baumannii strain MIC (μg/ml and μM) a Levofloxacin Ceftazidime Polymyxin B P307 P307 SQ-8C μg/ml μM μg/ml μM μg/ml μM μg/ml μM μg/ml μM 1791 6 16.6 128 234 0.25 0.19 750 221 62.5 14.5 1798 32 88.6 >1,024 >1,873 0.25 0.19 1,000 294 62.5 14.5 S5 6 16.6 64 117 0.25 0.19 2,000 588 125 29 ATCC 17978 0.075 0.21 6 11 0.25 0.19 750 221 125 29 Open in a separate window a Numbers in boldface indicate resistance to the antibiotics.

Techniques: Sequencing, Virus

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel Engineered Peptides of a Phage Lysin as Effective Antimicrobials against Multidrug-Resistant Acinetobacter baumannii

doi: 10.1128/AAC.02972-15

Figure Lengend Snippet: Structural predictions of PlyF307 and P307SQ-8C. (A) Full-length lysin PlyF307 is shown in blue, P307 fragment is shown in green, and the last eight amino acids (AEMELFLK) are shown in red. (B) P307SQ-8C is shown with P307 in green and the last eight amino acids (SQSRESQC) in purple. Structures were predicted using I-TASSER server (Zhang lab, University of Michigan) (31,–33).

Article Snippet: TABLE 2 A. baumannii strain MIC (μg/ml and μM) a Levofloxacin Ceftazidime Polymyxin B P307 P307 SQ-8C μg/ml μM μg/ml μM μg/ml μM μg/ml μM μg/ml μM 1791 6 16.6 128 234 0.25 0.19 750 221 62.5 14.5 1798 32 88.6 >1,024 >1,873 0.25 0.19 1,000 294 62.5 14.5 S5 6 16.6 64 117 0.25 0.19 2,000 588 125 29 ATCC 17978 0.075 0.21 6 11 0.25 0.19 750 221 125 29 Open in a separate window a Numbers in boldface indicate resistance to the antibiotics.

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel Engineered Peptides of a Phage Lysin as Effective Antimicrobials against Multidrug-Resistant Acinetobacter baumannii

doi: 10.1128/AAC.02972-15

Figure Lengend Snippet: Comparison of in vitro bactericidal activities of peptides. Bacteria were treated with 50 μg/ml of each peptide for 2 h at 22 to 25°C. Serial dilutions were plated for CFU counting. (A) The bactericidal activities of P307 and its variants P307AE-8 and P307SQ-8C against A. baumannii strains 1791, S5, and ATCC 17978. (B) The activities of P307, P307SQ-8C, and equimolar SQSRESQC peptide with or without P307 against strains 1791 and S5. (C) The activities of P307SQ-8C and a scramble variant P307CS-8 against strains 1791, S5, and ATCC 17978. The error bars show the standard deviations, and the black horizontal lines mark the limit of detection.

Article Snippet: TABLE 2 A. baumannii strain MIC (μg/ml and μM) a Levofloxacin Ceftazidime Polymyxin B P307 P307 SQ-8C μg/ml μM μg/ml μM μg/ml μM μg/ml μM μg/ml μM 1791 6 16.6 128 234 0.25 0.19 750 221 62.5 14.5 1798 32 88.6 >1,024 >1,873 0.25 0.19 1,000 294 62.5 14.5 S5 6 16.6 64 117 0.25 0.19 2,000 588 125 29 ATCC 17978 0.075 0.21 6 11 0.25 0.19 750 221 125 29 Open in a separate window a Numbers in boldface indicate resistance to the antibiotics.

Techniques: Comparison, In Vitro, Bacteria, Variant Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel Engineered Peptides of a Phage Lysin as Effective Antimicrobials against Multidrug-Resistant Acinetobacter baumannii

doi: 10.1128/AAC.02972-15

Figure Lengend Snippet: In vitro bactericidal spectra of P307 and P307SQ-8C. Different bacterial species (A. baumannii, B. anthracis, E. coli, P. aeruginosa, S. aureus, and K. pneumoniae) were treated with 50 μg/ml P307 or P307SQ-8C in 50 mM Tris-HCl (pH 7.5) for 2 h at 22 to 25°C to investigate the specificity of the peptides. Serial dilutions were plated for CFU counting. The error bars show the standard deviations, and the black horizontal line marks the limit of detection.

Article Snippet: TABLE 2 A. baumannii strain MIC (μg/ml and μM) a Levofloxacin Ceftazidime Polymyxin B P307 P307 SQ-8C μg/ml μM μg/ml μM μg/ml μM μg/ml μM μg/ml μM 1791 6 16.6 128 234 0.25 0.19 750 221 62.5 14.5 1798 32 88.6 >1,024 >1,873 0.25 0.19 1,000 294 62.5 14.5 S5 6 16.6 64 117 0.25 0.19 2,000 588 125 29 ATCC 17978 0.075 0.21 6 11 0.25 0.19 750 221 125 29 Open in a separate window a Numbers in boldface indicate resistance to the antibiotics.

Techniques: In Vitro

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Novel Engineered Peptides of a Phage Lysin as Effective Antimicrobials against Multidrug-Resistant Acinetobacter baumannii

doi: 10.1128/AAC.02972-15

Figure Lengend Snippet: Bactericidal activities of P307 and P307SQ-8C against A. baumannii strain 1791 at log phase, at late stationary phase, and in biofilm. (A) Bacteria in log phase and late stationary phase were treated with 50 μg/ml P307 or P307SQ-8C for 2 h at 22 to 25°C. (B) A. baumannii biofilms in PVC catheters were treated with 250 μg/ml of P307 or P307SQ-8C for 2 or 24 h at 22 to 25°C. Serial dilutions were plated for CFU counting. The error bars show the standard deviations, and the black horizontal line marks the limit of detection.

Article Snippet: TABLE 2 A. baumannii strain MIC (μg/ml and μM) a Levofloxacin Ceftazidime Polymyxin B P307 P307 SQ-8C μg/ml μM μg/ml μM μg/ml μM μg/ml μM μg/ml μM 1791 6 16.6 128 234 0.25 0.19 750 221 62.5 14.5 1798 32 88.6 >1,024 >1,873 0.25 0.19 1,000 294 62.5 14.5 S5 6 16.6 64 117 0.25 0.19 2,000 588 125 29 ATCC 17978 0.075 0.21 6 11 0.25 0.19 750 221 125 29 Open in a separate window a Numbers in boldface indicate resistance to the antibiotics.

Techniques: Bacteria