Journal: The Journal of Pathology

Article Title: Spatial transcriptomics exploration of the primary neuroblastoma microenvironment in archived FFPE samples unveils novel paracrine interactions

doi: 10.1002/path.6457

Figure Lengend Snippet: Identification of a spatially defined NRTN – GRFA2/RET interaction and experimental validation in NB cells. (A) Chord plot showing the number of predicted outgoing signaling interactions from the AC‐like cluster. Number of interactions with NE1 and NE2‐CA and examples of common interactions given. See supplementary material, Table for detailed results. (B) Spatial gene expression plots showing NRTN , GFRA2 , and RET expression in sections, as indicated. (C) Bar plot indicating percentages of NRTN expressing primary NB tumors with high (>90th percentile), intermediate, and low (<10th percentile) AC‐like signatures, as indicated. p values calculated using chi‐square test. (D) Kaplan–Meier survival plots comparing overall survival between NB patients with high (>90th percentile), intermediate (10th–90th percentile), and low (<10th percentile) gene expression, as indicated. p values calculated using the log rank test. (E) Western blot showing expression of GFRA2 in four different NB cell lines, as indicated. Normalized densitometry values indicated below each blot. (F, G) Time‐dependent effect of NRTN (100 ng/ml; n = 3) and/or selpercatinib (500 n m ; n = 3) application on (F) relative wound density (RWD; measured using a scratch/wound migration assay), and (G) cell growth (measured on Incucyte S3 system) on different NB cell lines, as indicated. p values for indicated timepoints calculated using an unpaired, two‐sided Student's t ‐test.

Article Snippet: Samples were subjected to western blotting analyses using the following antibodies: PITPNM3 (1:1,000) (Thermo Fisher Scientific, Cat# PA5‐21903, RRID:AB_11154802 ), GFRA2 (1:1,000) (Proteintech, Cat# 21973‐1‐AP, RRID:AB_11124728 ), and beta‐actin (1:10,000) (Cell Signaling Technology, Danvers, MA, USA; Cat# 4970, RRID:AB_2223172 ).

Techniques: Biomarker Discovery, Gene Expression, Expressing, Western Blot, Migration

Journal: Molecular Nutrition & Food Research

Article Title: Bioavailability of Glucoraphanin and Sulforaphane from High‐Glucoraphanin Broccoli

doi: 10.1002/mnfr.201700911

Figure Lengend Snippet: Summary table of the levels of unmetabolized glucoraphanin and its reduced analogue in plasma and urine after consumption of soups with different broccoli genotype

Article Snippet: Glucoraphanin (CAS 21414‐41‐5) (purity ≥ 95%) and glucoerucin (CAS 21973‐56‐8) (purity ≥ 97%) were purchased from Cayman Chemical and from Carl Roth, respectively.

Techniques: Clinical Proteomics, Concentration Assay

Journal: Molecular Nutrition & Food Research

Article Title: Bioavailability of Glucoraphanin and Sulforaphane from High‐Glucoraphanin Broccoli

doi: 10.1002/mnfr.201700911

Figure Lengend Snippet: Urinary excretion of intact glucoraphanin and glucoerucin (μmoles) in 24 h following consumption of Myb28 B/B (84 μmoles glucoraphanin per 300 g soup), Myb28 B/V (280 μmoles glucoraphanin per 300 g soup), and Myb28 V/V (452 μmoles glucoraphanin per 300 g soup) broccoli soups. Cumulative excretion of glucoraphanin/glucoerucin (A), and the total urinary excretion of glucoraphanin/glucoerucin (B) measured in urine by UPLC–MS/MS. Data ( n = 10) are represented as mean ± SD. Total urinary excretion data underwent square root transformation followed by analysis by one‐way analysis of variance with Tukey's honest significance test (*** p ˂ 0.0001 vs My28 B/B broccoli soup).

Article Snippet: Glucoraphanin (CAS 21414‐41‐5) (purity ≥ 95%) and glucoerucin (CAS 21973‐56‐8) (purity ≥ 97%) were purchased from Cayman Chemical and from Carl Roth, respectively.

Techniques: Tandem Mass Spectroscopy, Transformation Assay

Journal: Molecular Nutrition & Food Research

Article Title: Bioavailability of Glucoraphanin and Sulforaphane from High‐Glucoraphanin Broccoli

doi: 10.1002/mnfr.201700911

Figure Lengend Snippet: Percentage of glucoraphanin (A) and sulforaphane (B) excreted in the urine following consumption of the ingested dose of glucoraphanin in the broccoli soups, Myb28 B/B (84 μmoles glucoraphanin per 300 g soup), Myb28 B/V (280 μmoles glucoraphanin per 300 g soup), and Myb28 V/V (452 μmoles glucoraphanin per 300 g soup). Urine samples collected from participants (A–J) were analyzed for glucoraphanin, glucoerucin, sulforaphane, and its metabolites including sulforaphane, erucin‐NAC, sulforaphane‐cysteine, sulforaphane–cysteine‐glycine, and sulforaphane‐NAC.

Article Snippet: Glucoraphanin (CAS 21414‐41‐5) (purity ≥ 95%) and glucoerucin (CAS 21973‐56‐8) (purity ≥ 97%) were purchased from Cayman Chemical and from Carl Roth, respectively.

Techniques: