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Image Search Results
Journal: Communications biology
Article Title: FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior.
doi: 10.1038/s42003-024-07149-7
Figure Lengend Snippet: Fig. 3 | FOXP2 expression was decreased in tro- phoblasts under the H/R condition. A Images of immunofluorescence staining for CK-7 of isolated cells after 24 h, 72 h, and 96 h of culture. Scale bar = 50 μm. B The secretion of hCG in the super- natant of isolated cell culture medium after 24 h and 96 h of culture. C Images of immunofluorescence staining for CK-7, FOXP2, and Vimentin in isolated cells after 72 h of culture. Scale bar = 50 μm. D Western blot analysis for the protein level of CK-7 and Vimentin in isolated cells after 72 h of culture. E qRT-PCR analysis for the mRNA level of FOXP2 in primary trophoblasts. F Western blot analysis for the protein level of FOXP2 in primary trophoblasts. G Images of immunofluorescence staining for FOXP2 in primary trophoblasts. Scale bar = 100 μm. Data: mean ± SD. N = 3. *p value < 0.05, **p value < 0.01.
Article Snippet:
Techniques: Expressing, Staining, Isolation, Cell Culture, Western Blot, Quantitative RT-PCR
Journal: Communications biology
Article Title: FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior.
doi: 10.1038/s42003-024-07149-7
Figure Lengend Snippet: Fig. 4 | FOXP2 overexpression suppressed H/R-induced trophoblast apoptosis. A Western blot analysis for the protein level of FOXP2. B Cell viability was detected by MTT assay. C Western blot analysis for the protein levels of FOXP2, Bcl-2, Bcl-xl, and cleaved caspase-3. Data: mean ± SD. N = 3. *p value < 0.05, **p value < 0.01.
Article Snippet:
Techniques: Over Expression, Western Blot, MTT Assay
Journal: Communications biology
Article Title: FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior.
doi: 10.1038/s42003-024-07149-7
Figure Lengend Snippet: Fig. 5 | FOXP2 overexpression inhibited H/R- induced the decrease in the migration and inva- sion of trophoblasts. A Images of migrated and invaded trophoblasts. Scale bar = 100 μm. B The number of migrated and invaded trophoblasts. C The secretion of MMP-2 and MMP-9 in the supernatant of trophoblast culture medium. Data: mean ± SD. N = 3. *p value < 0.05, *p value < 0.01.
Article Snippet:
Techniques: Over Expression, Migration
Journal: Communications biology
Article Title: FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior.
doi: 10.1038/s42003-024-07149-7
Figure Lengend Snippet: Fig. 6 | FOXP2 overexpression restored H/R-induced the decrease in the tube formation of trophoblasts. A Visualization of tube formation of trophoblasts. Scale bar = 200 μm. B The number of tubes in the tube formation assay. C qRT-PCR analysis of the mRNA level of sFLt-1. Data: mean ± SD. N = 3. *pvalue < 0.05, **p value < 0.01.
Article Snippet:
Techniques: Over Expression, Tube Formation Assay, Quantitative RT-PCR
Journal: Communications biology
Article Title: FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior.
doi: 10.1038/s42003-024-07149-7
Figure Lengend Snippet: Fig. 7 | FOXP2 exerted a protective role in H/R- treated trophoblasts through transcriptionally upregulating LAMA4. A qRT-PCR analysis of the mRNA level of LAMA4. B Western blot analysis of the protein level of LAMA4. C Schematic diagram of LAMA4 promoter-luciferase constructs (left) and luciferase activity was determined by dual luciferase reporter assay in trophoblasts co-transfected with promoter constructs and ovFOXP2/Ev (right). D ChIP-PCR analysis for determining the FOXP2 binding to LAMA4 promoter. E Western blot ana- lysis of the protein level of LAMA4. F Images of migrated and invaded trophoblasts. Scale bar = 100 μm. G The number of migrated and invaded tro- phoblasts. Data: mean ± SD. N = 3. *p value < 0.05, **p value < 0.01.
Article Snippet:
Techniques: Quantitative RT-PCR, Western Blot, Luciferase, Construct, Activity Assay, Reporter Assay, Transfection, Binding Assay, Lysis