Journal: The Journal of comparative neurology

Article Title: Expression of Islet1 Marks the Sensory and Neuronal Lineages in the Mammalian Inner Ear

doi: 10.1002/cne.20257

Figure Lengend Snippet: Expression of Islet1 (Isl1) in the inner ear neuronal lineage from embryonic day (E) 8.5 to E9.5. A–L: A vertical section from E8.5 (A–D) and a transverse ventral section from E9.5 (E–L) embryos were triply stained with antibodies against Pax2 (A,E,I, magenta), NeuroD (B,F,J, green), and Isl1 (C,G,K, red). D,H,L: Overlays of NeuroD and Isl1 staining. I,J,K,L: Higher magnified images of E,F,G,H, respectively. For E8.5 vertical section, dorsal is at the top, and for E9.5 transverse section, anterior is at the top. Medial is at the left for both the sections. VCG, vestibular–cochlear ganglion neurons. Scale bars = 50 μm in A (applies to A–D), E (applies to E–H), I (applies to I–L).

Article Snippet: For VCG neurons, we used an antibody (Tuj1) against the neuronal-specific β-tubulin ( Lee et al., 1990 ) that recognizes delaminated VCG neurons and antibody against NeuroD (Santa Cruz) that is expressed transiently in VCG precursors and in delaminated VCG neuroblasts.

Techniques: Expressing, Staining

Journal: The Journal of comparative neurology

Article Title: Expression of Islet1 Marks the Sensory and Neuronal Lineages in the Mammalian Inner Ear

doi: 10.1002/cne.20257

Figure Lengend Snippet: Expression of Islet1 (Isl1) in the inner ear neuronal lineage at embryonic day (E) 10.5. A–D: Adjacent vertical (A,B) or transverse (C,D) E10.5 sections were stained for Isl1 (A,C) or Tuj-1 (C,D). Arrows in A and C delimit the expression domain of Isl1 in the otic epithelium at this stage. E–H: Transverse (E,F) and vertical (G,H) sections were triply labeled for Pax2 (E,G, magenta), Isl1 (F,H, red), and NeuroD (F,H, green). F,H: The staining for Isl1 and NeuroD was overlaid. VCG, vestibular–cochlear ganglion neurons. Scale bars = 50 μm in A (applies to A,B), C (applies to C,D), E (applies to E,F), G (applies to G,H).

Article Snippet: For VCG neurons, we used an antibody (Tuj1) against the neuronal-specific β-tubulin ( Lee et al., 1990 ) that recognizes delaminated VCG neurons and antibody against NeuroD (Santa Cruz) that is expressed transiently in VCG precursors and in delaminated VCG neuroblasts.

Techniques: Expressing, Staining, Labeling

Journal: The Journal of comparative neurology

Article Title: Expression of Islet1 Marks the Sensory and Neuronal Lineages in the Mammalian Inner Ear

doi: 10.1002/cne.20257

Figure Lengend Snippet: Islet1 (Isl1) expression in the vestibular-cochlear ganglion neurons and in the otic epithelium at embryonic day (E) 11.5. A–D: Adjacent otocyst sections at E11.5 (A,B and C,D) were stained with an antibody against Isl1 (Isl1, A,C) or neuronal β-tubulin (Tuj1, B,D). C,D: Higher magnifications of the ventral region in A and B, respectively. The green signal in A–D is from green fluorescent protein (GFP) expressed under the control of Math1 enhancers (Chen et al., 2002). Arrows in A and C indicate the boundaries of the Isl1-expressing ventral–medial region of the otocyst. Arrowheads in C and D indicate cells expressing the nucleus-localized GFP under the control of Math1 enhancers within the otocyst epithelium. AE,F: A transverse section triply stained for Isl1 (E,F, red), NeuroD (E,F, green), and Pax2 (E,F, magenta). E: The staining of Isl1 and NeuroD was overlaid to indicate specific staining for each (red for Isl1 and green for NeuroD) or for double labeling (yellow). F: All three labels were overlaid to indicate the relative expression domains of each gene. Note that the magenta colored Pax2 staining covers over other staining. Only complementary staining to Pax2 staining of Isl1 and NeuroD can be showed in F. Arrows in E,F indicate the Isl1-expressing domain in the otic epithelium that is negative for NeuroD staining. VCG, vestibular–cochlear ganglion neurons. Scale bars = 50 μm A (applies to A,B), C (applies to C,D), E (applies to E,F).

Article Snippet: For VCG neurons, we used an antibody (Tuj1) against the neuronal-specific β-tubulin ( Lee et al., 1990 ) that recognizes delaminated VCG neurons and antibody against NeuroD (Santa Cruz) that is expressed transiently in VCG precursors and in delaminated VCG neuroblasts.

Techniques: Expressing, Staining, Control, Labeling