Journal: Virus Research

Article Title: Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus

doi: 10.1016/j.virusres.2008.04.015

Figure Lengend Snippet: (a) Genome organization of TCoV. Diagram shows putative ORFs. UTRs, leader (L), and TRS are not to scale. Above, the genome organization of TCoV are shown the predicted five sgRNA in relative sizes. Genome organization of IBV-Beaudette ( NC_001451 ) is displayed below for comparison with that of TCoV. (b) Mapping of TCoV polyprotein. Predicted non-structure proteins (nsp2–nsp16) for pp1a are shown in relative sizes (bottom panel). Nsp1 is missing from TCoV and nsp11 contains only 23 aa. The sequence is for ATCC isolate (accession number EU022526 ).

Article Snippet: The sequence of polymerase gene 1 of TCoV isolate ATCC contained 20,441-nts, excluding the 5′ UTR.

Techniques: Comparison, Sequencing

Journal: Virus Research

Article Title: Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus

doi: 10.1016/j.virusres.2008.04.015

Figure Lengend Snippet: Sequence identity of TCoV-ATCC nsps with other coronaviruses

Article Snippet: The sequence of polymerase gene 1 of TCoV isolate ATCC contained 20,441-nts, excluding the 5′ UTR.

Techniques: Sequencing

Journal: Virus Research

Article Title: Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus

doi: 10.1016/j.virusres.2008.04.015

Figure Lengend Snippet: Northern blotting of total RNA isolated from TCoV infected turkey small intestines. Total RNA (10 μg) was isolated from mock or ATCC infected turkey small intestines 3 days post infection, separated on 1% agarose gel, transferred onto nitrocellulose membrane, and detected with 32P labeled PCR probe corresponding to N gene. The sizes (kb) on the right indicate the predicted size of genomic and subgenomic RNA. X indicates assumed DI RNA.

Article Snippet: The sequence of polymerase gene 1 of TCoV isolate ATCC contained 20,441-nts, excluding the 5′ UTR.

Techniques: Northern Blot, Isolation, Infection, Agarose Gel Electrophoresis, Membrane, Labeling

Journal: Virus Research

Article Title: Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus

doi: 10.1016/j.virusres.2008.04.015

Figure Lengend Snippet: Sequences flanking TRS region for TCoV sgRNA. The partial sequences display each sgRNA for ATCC isolate. For each sgRNA, partial genomic leader (gL) and body (gS, gE, gM, g5, and gN) sequences are displayed above and below sgRNA. The star (*) indicates identical nucleotide and the box indicates TRS region where template switch is assumed to occur.

Article Snippet: The sequence of polymerase gene 1 of TCoV isolate ATCC contained 20,441-nts, excluding the 5′ UTR.

Techniques: