2016 addgene Search Results


paav u6 sgrna hsyn cre  (Addgene inc)
91
Addgene inc paav u6 sgrna hsyn cre
Paav U6 Sgrna Hsyn Cre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1+(Plasmid+%2392120)/10__7554_slash_elife__50712-356-26-38
Average 91 stars, based on 1 article reviews
paav u6 sgrna hsyn cre - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier
stargazin gfp lovpep sequence  (Addgene inc)
93
Addgene inc stargazin gfp lovpep sequence
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Stargazin Gfp Lovpep Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/Stargazin-GFP-LOVpep+(Plasmid+%2380406)/pm35254388-261-11-14
Average 93 stars, based on 1 article reviews
stargazin gfp lovpep sequence - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
2016 • gpi 2xmcherry  (Addgene inc)
92
Addgene inc 2016 • gpi 2xmcherry
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
2016 • Gpi 2xmcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/GPI_2xmCherry+(Plasmid+%23127812)/pm38704407-273-78-87
Average 92 stars, based on 1 article reviews
2016 • gpi 2xmcherry - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
thomas jessell  (Addgene inc)
91
Addgene inc thomas jessell
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Thomas Jessell, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/RabV+CVS-N2c(deltaG)-mCherry-P2A-FlpO+(Plasmid+%2373471)/pm34478630-290-7-9
Average 91 stars, based on 1 article reviews
thomas jessell - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier
addgene 73452  (Addgene inc)
90
Addgene inc addgene 73452
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Addgene 73452, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/J8AAV-HDR+Ctnnb1%2E1+(Plasmid+%2373452)/pm33908623-224-37-37
Average 90 stars, based on 1 article reviews
addgene 73452 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
addgene 65992  (Addgene inc)
93
Addgene inc addgene 65992
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Addgene 65992, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/J23106_AB+(Plasmid+%2365992)/pm39919271-256-16-50
Average 93 stars, based on 1 article reviews
addgene 65992 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
paper n a recombinant dna plasmid mouse sgrna library brie in lenticrisprv2  (Addgene inc)
94
Addgene inc paper n a recombinant dna plasmid mouse sgrna library brie in lenticrisprv2
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Paper N A Recombinant Dna Plasmid Mouse Sgrna Library Brie In Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/Mouse+CRISPR+Knockout+Pooled+Library+(Brie)+(Pooled+Library+%2373632%2C+%2373633%2C+%2373633-LV)/pm34879220-249-59-75
Average 94 stars, based on 1 article reviews
paper n a recombinant dna plasmid mouse sgrna library brie in lenticrisprv2 - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
pcag 1bpnls cas9 1bpnls 2agfp  (Addgene inc)
96
Addgene inc pcag 1bpnls cas9 1bpnls 2agfp
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Pcag 1bpnls Cas9 1bpnls 2agfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/gRNA_Cloning+Vector+(Plasmid+%2341824)/pm30661960-195-110-140
Average 96 stars, based on 1 article reviews
pcag 1bpnls cas9 1bpnls 2agfp - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
v farese  (Addgene inc)
93
Addgene inc v farese
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
V Farese, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/BFP-KDEL+(Plasmid+%2349150)/pm31178403-340-0-7
Average 93 stars, based on 1 article reviews
v farese - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
sgrna vector pcrispria v2  (Addgene inc)
96
Addgene inc sgrna vector pcrispria v2
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Sgrna Vector Pcrispria V2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/pCRISPRia-v2+(Plasmid+%2384832)/pmc06813890-1169-30-33
Average 96 stars, based on 1 article reviews
sgrna vector pcrispria v2 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
rabies n2c enva dsred andrew murray lab  (Addgene inc)
90
Addgene inc rabies n2c enva dsred andrew murray lab
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Rabies N2c Enva Dsred Andrew Murray Lab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/RabV+CVS-N2c(deltaG)-hChR2-YFP+(Plasmid+%2373465)/pm35063074-305-190-200
Average 90 stars, based on 1 article reviews
rabies n2c enva dsred andrew murray lab - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pcag cyrfp1  (Addgene inc)
93
Addgene inc pcag cyrfp1
Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and <t>LOVpep)</t> injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.
Pcag Cyrfp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2016+addgene/pBS-crb43%2E2+(Plasmid+%2320168)/10__1523_slash_jneurosci__0046___20__2020-40-32-33
Average 93 stars, based on 1 article reviews
pcag cyrfp1 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Journal: The Journal of cell biology

Article Title: Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling.

doi: 10.1083/jcb.202105107

Figure Lengend Snippet: Figure 5. Junction elongation induces Rho flares. (A) Graph showing that calcium flash (GCaMP6m) follows junction elongation (verted-to-vertex length) and precedes Rho-mediated junction contraction and stabilization of length. Time 0 s represents start of Rho flare. Shaded region represents SEM; n = 16 flares, 13 embryos, 10 experiments. (B) Schematic of mosaic injection and regional activation of Rho-mediated contractility using optogenetics. Top: Schematic showing the mosaic injection of optogenetic constructs (prGEF and LOVpep) injected into one cell, while active Rho probe is injected in both cells at two-cell stage. Bottom: Regional activation of RhoA using optogenetics in region of stimulation (shaded gray box) and quantification of junction length and frequency of Rho flares in region of observation (dotted blue box). (C) Cell view of an embryo mosaically-expressing prGEF (2xPDZ-YFP-LARG(DH), yellow) and active Rho probe (mCherry-2xrGBD, gray). (C9) Cell view of Rho probe in the embryo from C. Regional stimulation (white dashed box) induces Rho flares in areas neighboring the region of stimulation (yellow arrowheads). (D) Quantification of Rho flares in the area outside the region of stimulation shown in C9. Frequency of Rho flares occurring pre-stimulation (0–300 s) and post-stimulation (600–900 s) are matched for color and shape. Significance calculated using unpaired two-tailed t test; n = 6 embryos, 4 experiments. (E) Time-lapse montage of a junction highlighted in C9 (yellow box) expressing active Rho probe (mCherry- 2xrGBD). Following optogenetic stimulation (indicated by gray shaded box), an increase in junction length precedes Rho flare activation (yellow arrowhead). Junction length measured from vertex to vertex is indicated under each panel.

Article Snippet: Stargazin-GFP-LOVpep (referred to as GFPLOVpep) was generated by PCR amplifying the Stargazin-GFP- LOVpep sequence (Addgene plasmid #80406; Wagner and Glotzer, 2016) and ligating into the ClaI site in pCS2+.

Techniques: Injection, Activation Assay, Optogenetics, Construct, Expressing, Two Tailed Test