201145a Search Results


maxpar x8 antibody labeling kit  (fluidigm)
94
fluidigm maxpar x8 antibody labeling kit
Optimizing antibody staining and resolution in BAL cells (A) Antibody staining was tested on fresh PBMCs followed by <t>MaxPar</t> Fix PERM incubation with Ir 191/193 (no fix) after fixation with 2% and 4% paraformaldehyde (PFA) or BD FACS lyse buffer followed by MaxPar Fix PERM incubation with Ir 191/193 for antibodies CD4 clone RPA-T4 (row 1), CD56 clone NCAM16.2 (row 2), CD16 clone 3G8 and CD11b clone ICRF44 (row 3). Staining after fixative treatment reduced the resolution of these antibody clones compared to fresh cells. (B) Titrations of surface marker CD206 and intra-cellular marker IL1ß on BAL cells highlights the importance of titrating markers on both stimulated and unstimulated cells. (C) BAL or PBMCs were stained with the full antibody cocktail (stained) and compared to unstained controls (Ir 191/193 staining only) to determine true positive signal for markers CD19 and CD32 (row 1), CD19 and CD40 (row 2), CD11c and CD89 (row 3).
Maxpar X8 Antibody Labeling Kit, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/maxpar x8 antibody labeling kit/product/fluidigm
Average 94 stars, based on 1 article reviews
maxpar x8 antibody labeling kit - by Bioz Stars, 2026-03
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Optimizing antibody staining and resolution in BAL cells (A) Antibody staining was tested on fresh PBMCs followed by MaxPar Fix PERM incubation with Ir 191/193 (no fix) after fixation with 2% and 4% paraformaldehyde (PFA) or BD FACS lyse buffer followed by MaxPar Fix PERM incubation with Ir 191/193 for antibodies CD4 clone RPA-T4 (row 1), CD56 clone NCAM16.2 (row 2), CD16 clone 3G8 and CD11b clone ICRF44 (row 3). Staining after fixative treatment reduced the resolution of these antibody clones compared to fresh cells. (B) Titrations of surface marker CD206 and intra-cellular marker IL1ß on BAL cells highlights the importance of titrating markers on both stimulated and unstimulated cells. (C) BAL or PBMCs were stained with the full antibody cocktail (stained) and compared to unstained controls (Ir 191/193 staining only) to determine true positive signal for markers CD19 and CD32 (row 1), CD19 and CD40 (row 2), CD11c and CD89 (row 3).

Journal: STAR Protocols

Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

doi: 10.1016/j.xpro.2024.103463

Figure Lengend Snippet: Optimizing antibody staining and resolution in BAL cells (A) Antibody staining was tested on fresh PBMCs followed by MaxPar Fix PERM incubation with Ir 191/193 (no fix) after fixation with 2% and 4% paraformaldehyde (PFA) or BD FACS lyse buffer followed by MaxPar Fix PERM incubation with Ir 191/193 for antibodies CD4 clone RPA-T4 (row 1), CD56 clone NCAM16.2 (row 2), CD16 clone 3G8 and CD11b clone ICRF44 (row 3). Staining after fixative treatment reduced the resolution of these antibody clones compared to fresh cells. (B) Titrations of surface marker CD206 and intra-cellular marker IL1ß on BAL cells highlights the importance of titrating markers on both stimulated and unstimulated cells. (C) BAL or PBMCs were stained with the full antibody cocktail (stained) and compared to unstained controls (Ir 191/193 staining only) to determine true positive signal for markers CD19 and CD32 (row 1), CD19 and CD40 (row 2), CD11c and CD89 (row 3).

Article Snippet: Maxpar X8 antibody labeling kit, 145Nd , Standard BioTools , Cat# 201145A.

Techniques: Staining, Incubation, Clone Assay, Marker

Journal: STAR Protocols

Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

doi: 10.1016/j.xpro.2024.103463

Figure Lengend Snippet:

Article Snippet: Maxpar X8 antibody labeling kit, 145Nd , Standard BioTools , Cat# 201145A.

Techniques: Recombinant, Staining, Saline, Software, Antibody Labeling, Spectrophotometry, Hood, Microscopy, Membrane, Sterility, Transferring

Mass cytometry chemokine receptor cocktail

Journal: STAR Protocols

Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

doi: 10.1016/j.xpro.2024.103463

Figure Lengend Snippet: Mass cytometry chemokine receptor cocktail

Article Snippet: Maxpar X8 antibody labeling kit, 145Nd , Standard BioTools , Cat# 201145A.

Techniques: Mass Cytometry, Concentration Assay, Staining

Mass cytometry surface antibody cocktail

Journal: STAR Protocols

Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

doi: 10.1016/j.xpro.2024.103463

Figure Lengend Snippet: Mass cytometry surface antibody cocktail

Article Snippet: Maxpar X8 antibody labeling kit, 145Nd , Standard BioTools , Cat# 201145A.

Techniques: Mass Cytometry, Concentration Assay, Staining

Mass cytometry intra-cellular antibody cocktail

Journal: STAR Protocols

Article Title: Protocol to phenotype and quantify mycobacteria-specific myeloid cells from human airways by mass cytometry

doi: 10.1016/j.xpro.2024.103463

Figure Lengend Snippet: Mass cytometry intra-cellular antibody cocktail

Article Snippet: Maxpar X8 antibody labeling kit, 145Nd , Standard BioTools , Cat# 201145A.

Techniques: Mass Cytometry, Concentration Assay