Journal: Journal of Neurochemistry

Article Title: Unveiling the Molecular Mechanism of Intestinal Metabolite para ‐Cresol in Modulating Neuroinflammation and Synaptic Dysfunction: Implications for Autism Spectrum Disorder

doi: 10.1111/jnc.70457

Figure Lengend Snippet: Effects of p‐ Cresol on DITNC1 astrocytes. (A) Experimental workflow: (B–E) Representative images of astroglial cells incubated with vehicle (Ctrl), and concentrations of 50, 150, or 300 μM of p ‐Cresol for 24 h following CellROX (green) staining. Cell nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F) Quantitative results of the DAPI + astrocyte cell count 24 h after stimulation with either vehicle or different p ‐Cresol concentrations. (G) Effects of p‐ Cresol on oxidative stress in DITNC1 cells measured using CellROX staining. Results are expressed as mean ± SEM values. Statistical analyses were conducted using two‐way ANOVA with Tukey's multiple comparison tests: * p < 0.05 vs. Ctrl, *** p < 0.001 vs. Ctrl, $$ p < 0.01 vs. p‐ Cresol 50 μM.

Article Snippet: DITNC1 (RRID:CVCL_0247, CRL‐2005, ATCC) rat astrocyte cells were cultured in Dulbecco's modified eagle medium (DMEM) (#D6546, Sigma) supplemented with 10% fetal bovine serum (FBS) (#2473339RP, Gibco), 2% L‐glutamine (#G7513, Sigma), and 1% penicillin/streptomycin (Pen/Strep) (P4333, Sigma).

Techniques: Incubation, Staining, Cell Characterization, Comparison

Journal: Journal of Neurochemistry

Article Title: Unveiling the Molecular Mechanism of Intestinal Metabolite para ‐Cresol in Modulating Neuroinflammation and Synaptic Dysfunction: Implications for Autism Spectrum Disorder

doi: 10.1111/jnc.70457

Figure Lengend Snippet: Effects of p ‐Cresol on gene expression of DITNC1 astrocytes. DITNC1 cells were treated with vehicle, 50 or 150 μM of p‐ Cresol for 24 h, then the total RNA was extracted, and qPCR was conducted. (A) Data are presented as fold change normalized over the Ctrl group mean value and calibrated over GAPDH/APOE housekeeping genes. The experimental conditions were tested in triplicate, and data were expressed as mean ± SEM values and analyzed by one‐way ANOVA (B). The colorimetric scale was used to show up‐ (green) or down‐ (red) regulated genes; * p < 0.05; *** p < 0.001; $$ p < 0.01 vs. p‐ Cresol 50 μM; #0.10 > p > 0.05.

Article Snippet: DITNC1 (RRID:CVCL_0247, CRL‐2005, ATCC) rat astrocyte cells were cultured in Dulbecco's modified eagle medium (DMEM) (#D6546, Sigma) supplemented with 10% fetal bovine serum (FBS) (#2473339RP, Gibco), 2% L‐glutamine (#G7513, Sigma), and 1% penicillin/streptomycin (Pen/Strep) (P4333, Sigma).

Techniques: Gene Expression

Journal: Cell Reports

Article Title: Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells

doi: 10.1016/j.celrep.2020.107691

Figure Lengend Snippet:

Article Snippet: NextSeq® 500/550 High Output 75 cycle sequencing kit v2 , Illumina , Cat# FC-404-2005.

Techniques: Virus, Recombinant, Magnetic Beads, Modification, Knock-Out, In Vitro, Sequencing, Reverse Transcription, Staining, Library Quantification, Illumina Sequencing, Bicinchoninic Acid Protein Assay, cDNA Synthesis, TaqMan Assay, Software, Sterility

Journal: bioRxiv

Article Title: Non-enzymatic assimilation of organosulfur compounds at the interface of geochemistry and biochemistry

doi: 10.64898/2026.02.18.706717

Figure Lengend Snippet: (A) The intracellular Fenton chemistry drives the ROS-driven degradation of DMSO, resulting in MSA, sulfite, CH 4 and methanol, thereby allowing for a non-enzymatic metabolic pathway of sulfur and carbon assimilation. (B) M. hispanicum respires carbon from 13 C-DMSO. Both 13 C/ 12 C ratios and formation rates of the formed CO 2 are shown, from media controls (circles) and corresponding M. hispanicum cultures (squares), from unlabeled (blue) to 13 C-labeled DMSO (red). Cartoon generated with BioRender.com .

Article Snippet: Pre-cultures of Methylobacterium hispanicum were cultivated in modified DSMZ 1629 medium, harboring 1.61 g L -1 NH4Cl, 0.2 g L -1 MgSO 4 · 7 H 2 O, 2.4 g L -1 K2HPO4, 1.1 g L -1 NaH2PO4 · 2 H2O, 4.5 mg L -1 ZnSO4 · 7 H2O, 3 mg CoCl2 · 6 H2O, 0.64 mg L -1 MnCl2 · 4 H2O, 1 mg L -1 H3BO3, 0.4 mg L -1 Na2MoO4 · 2 H2O, 0.3 mg L -1 CuSO4 · 2 H2O, and 3 mg L -1 CaCl2 · 6 H2O, which was further supplemented with 5 mL L -1 methanol, 30 mM sodium citrate and 10 mM FeCl 2 .

Techniques: Labeling, Generated

Journal: Environmental Microbiology

Article Title: Comparative Physiology and Genomics of Thermincola and Carboxydocella Strains and Description of Two Novel Isolates

doi: 10.1111/1462-2920.70283

Figure Lengend Snippet: (a) Maximum likelihood phylogenetic tree based on the concatenation of 120 single‐copy genes. Different colours represent different families (GTDB r220). The tree scale bar represents 0.1 substitutions per site. The numbers in nodes represent bootstrap values, and accession numbers are given in parentheses. Thermoanaerobacter kivui was used as an outgroup and root of the tree. (b) Average nucleotide identity comparisons between publicly available Thermincola strains and strain AZ34E. (c) Average nucleotide identity comparisons between publicly available Carboxydocella strains and strain AZ29I.

Article Snippet: In addition, digital DNA–DNA hybridisation (dDDH) values (and confidence intervals) between the genomes of AZ29I, AZ34E, Thermincola carboxydiphila (DSM 17129 T ) and their closest relatives were calculated using the Type (Strain) Genome Server ( https://tygs.dsmz.de ) and the recommended settings of GGDC v4.0 (Meier‐Kolthoff et al. , ; Yoon et al. ).

Techniques:

Journal: Environmental Microbiology

Article Title: Comparative Physiology and Genomics of Thermincola and Carboxydocella Strains and Description of Two Novel Isolates

doi: 10.1111/1462-2920.70283

Figure Lengend Snippet: Phylogenetic comparison of CODHs detected in the Themincola and Carboxydocella genomes. The tree was constructed with the LG + G4 model in IQ‐TREE v2.0.6 and visualised in iTOL v6.8.1. CODH sequences of Thermincola sp. strain AZ34E and Carboxydocella sp. strain AZ29I are depicted in bold. The CODHs of Carboxydothermus hydrogenoformans were used as reference points for functional predictions. The tree scale bar represents 1 substitution per site. The circles in nodes represent bootstrap values > 80. The genomic structure of cooS gene clusters encoding the different CODHs in Thermincola sp. strain AZ34E and Carboxydocella sp. strain AZ291 are shown in the boxes on the top right corner. Gene abbreviations: acsB, Acetyl‐CoA synthase; acsC, corrinoid iron–sulfur protein large subunit; acsD, corrinoid iron–sulfur protein small subunit; acsE, methyltransferase A; ATPase, AAA family ATPase; cooA, CO‐dependent transcriptional activator; cooC, CODH chaperone; cooF, ferredoxin‐like electron transfer Fe‐S protein; cooMKLXUH, sub‐units of the energy‐converting hydrogenase; cooS, CO‐dehydrogenase; FNOR, NAD/FAD oxidoreductase; hp, hypothetical protein; hypA, hydrogenase maturation protein; nqrF, Na(+)‐translocating NADH‐quinone reductase subunit F; TR, transcription regulator; YlmC, YlmC/YmxH family sporulation protein; YpiB, YpiB family protein.

Article Snippet: In addition, digital DNA–DNA hybridisation (dDDH) values (and confidence intervals) between the genomes of AZ29I, AZ34E, Thermincola carboxydiphila (DSM 17129 T ) and their closest relatives were calculated using the Type (Strain) Genome Server ( https://tygs.dsmz.de ) and the recommended settings of GGDC v4.0 (Meier‐Kolthoff et al. , ; Yoon et al. ).

Techniques: Comparison, Construct, Functional Assay