Journal: Scientific reports

Article Title: LncRNA HOXA-AS3 promotes cell proliferation and invasion via targeting miR-218-5p/FOXP1 axis in osteosarcoma.

doi: 10.1038/s41598-024-67596-4

Figure Lengend Snippet: Figure 3. LncRNA HOXA-AS3 interacts with miR-218-5p in osteosarcoma cells. (A) Potential binding sites between miR-218-5p and lncRNA HOXA-AS3 and FOXP1. (B) RT-PCR data showed that miR-218-5p inhibitors decreased the expression of miR-218-5p. In addition, miR-218-5p mimic transfection increased the miR-218-5p expression levels. (C) Dual luciferase reporter assays showed that miR-218-5p interacted with lncRNA HOXA-AS3. miR-218-5p regulated luciferase activity in HOXA-AS3 wild-type group. (D) Dual luciferase reporter assays showed that miR-218-5p interacted with and regulated FOXP1. **p < 0.01 versus control group. (E) Western blotting analysis data showed that lncRNA HOXA-AS3 and miR-218-5p regulate the expression of FOXP1 in osteosarcoma cells. WT: wild type; MUT: mutant.

Article Snippet: The primary antibody FOXP1 (1:2000, #2005, Cell Signaling Technology) were used to incubate the membrane at cold room overnight.

Techniques: Binding Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection, Luciferase, Activity Assay, Control, Western Blot, Mutagenesis

Journal: Scientific reports

Article Title: LncRNA HOXA-AS3 promotes cell proliferation and invasion via targeting miR-218-5p/FOXP1 axis in osteosarcoma.

doi: 10.1038/s41598-024-67596-4

Figure Lengend Snippet: Figure 4. Downregulation of lncRNA HOXA-AS3 reduces colony formation via miR-218-5p and FOXP1. (A) Colony formation assays were performed to measure the colony formation in osteosarcoma cells co-transfected with sh-HOXA-AS3 and pcDNA FOXP1, or sh-HOXA-AS3 and miR-218 inhibitors. (B) Quantitative data are shown for colony formation. ***p < 0.001 versus control group. #p < 0.05; ##p < 0.01 versus sh-HOXA-AS3 alone.

Article Snippet: The primary antibody FOXP1 (1:2000, #2005, Cell Signaling Technology) were used to incubate the membrane at cold room overnight.

Techniques: Transfection, Control

Journal: Scientific reports

Article Title: LncRNA HOXA-AS3 promotes cell proliferation and invasion via targeting miR-218-5p/FOXP1 axis in osteosarcoma.

doi: 10.1038/s41598-024-67596-4

Figure Lengend Snippet: Figure 5. Downregulation of lncRNA HOXA-AS3 reduces cell migration via miR-218-5p and FOXP1. (A) Wound healing assays were performed to measure migratory ability in osteosarcoma cells co-transfected with sh-HOXA-AS3 and pcDNA FOXP1, or sh-HOXA-AS3 and miR-218 inhibitors. (B) Quantitative data are shown for colony formation. **p < 0.01; ***p < 0.001 versus control group. #p < 0.05; ##p < 0.01 versus sh-HOXA-AS3 alone.

Article Snippet: The primary antibody FOXP1 (1:2000, #2005, Cell Signaling Technology) were used to incubate the membrane at cold room overnight.

Techniques: Migration, Transfection, Control

Journal: Scientific reports

Article Title: LncRNA HOXA-AS3 promotes cell proliferation and invasion via targeting miR-218-5p/FOXP1 axis in osteosarcoma.

doi: 10.1038/s41598-024-67596-4

Figure Lengend Snippet: Figure 6. Downregulation of lncRNA HOXA-AS3 reduces cell invasion via miR-218-5p and FOXP1. (A) Transwell assays were performed to measure cell invasive ability in osteosarcoma cells co-transfected with sh-HOXA-AS3 and pcDNA FOXP1, or sh-HOXA-AS3 and miR-218 inhibitors. (B) Quantitative data are shown for colony formation. ***p < 0.001 versus control group. #p < 0.05; ##p < 0.01 versus sh-HOXA-AS3 alone.

Article Snippet: The primary antibody FOXP1 (1:2000, #2005, Cell Signaling Technology) were used to incubate the membrane at cold room overnight.

Techniques: Transfection, Control