Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: DXS253E expression is increased in different types of tumors including colorectal cancer (CRC). A Pan-cancer analysis of DXS253E expression levels in human tumors according to the Cancer Genome Atlas (TCGA) dataset. B Expression of DXS253E in cancer and para-cancer paired tissues based on TCGA dataset. C, D Comparison of the expression levels of DXS253E in colon adenocarcinoma (COAD) (C) or rectum adenocarcinoma (READ) (D) and normal tissues from TCGA’s database. E Gene Expression Omnibus database (GEO) analysis of DXS253E expression in CRC tissues. F qRT-PCR assay of DXS253E mRNA expression levels in eight pairs of CRC and adjacent tissues from our experimental cohort. G Representative images of DXS253E expression in CRC tissues and matched normal tissues. Original magnifications 100× and 200× (inset panels). * P < 0.05, ** P < 0.01, *** P < 0.001, ns, no significance

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques: Expressing, Comparison, Gene Expression, Quantitative RT-PCR

Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: High DXS253E expression indicates aggressive clinical features and poor prognosis for CRC patients. A Association between DXS253E expression and clinical characteristics. B, C Effects of DXS253E level on overall survival (OS) and disease-specific survival (DSS) in COAD and READ. D Forest plot of univariate Cox regression analysis of DXS253E mRNA expression with OS and DSS in CRC with different clinicopathological features. E Forest plot of multivariate Cox regression analysis of DXS253E mRNA expression with OS and DSS in CRC with different clinicopathological features. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: Univariable and multivariable analysis for OS in CRC patients with TCGA cohort

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques:

Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: Univariable and multivariable analysis for DSS in CRC patients with TCGA cohort

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques:

Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: Mutation and methylation analysis of DXS253E in CRC. A Alteration frequency of the DXS253E gene across various cancers analyzed using the cBioPortal web resource. B Differential somatic mutations identified in CRC between low and high DXS253E expression groups. C Correlation between DXS253E mRNA expression level and methylation level. D Kaplan-Meier survival curves showing six methylation sites in the DXS253E gene

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques: Mutagenesis, Methylation, Expressing

Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: Functional enrichment analysis of differentially expressed genes (DEGs) according to DXS253E expression level in CRC. A Volcano plot for DEGs between low DXS253E and high DXS253E expression groups. B Heatmap showing the top 10 DEGs between low and high DXS253E-expression groups. C GO enrichment analysis of DXS253E-associated DEGs. D KEGG enrichment analysis of DXS253E-associated DEGs. E GSEA of relevant signaling pathways in CRC tissues based on DXS253E-related DEGs. F Volcano plot of co-expressed genes correlated with DXS253E expression using the LinkedOmics web resource. G Heatmaps of the top 50 genes that are positively or negatively associated with DXS253E. H Venn diagram of the number of intersections between DXS253E DEGs and co‐expressed genes in CRC. I Enrichment analysis of overlapping genes analyzed by the Metascape web resource

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques: Functional Assay, Expressing, Protein-Protein interactions

Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: DXS253E expression is associated with multiple immune-related genes, and immune cell infiltration occurs in CRC. A Correlation of DXS253E with immunomodulatory genes in pan-cancer. B Heatmap of DXS253E expression with various tumor microenvironment cells in five independent datasets from the Tumor Immune Single-cell Hub (TISCH) database. C DXS253E expression in immune cells according to the Gene Expression Omnibus (GEO) GSE108989 and GSE136394 datasets. D Correlation between DXS253E and immune cell infiltration. E, F Relationship of DXS253E expression with the infiltration level of NK, NK CD56bright, eosinophil, Tcm, T helper, and Th2 cells using scatter plots (E) and box plots (F). * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques: Expressing, Gene Expression

Journal: Cancer Cell International

Article Title: Integrated profiling identifies DXS253E as a potential prognostic marker in colorectal cancer

doi: 10.1186/s12935-024-03403-4

Figure Lengend Snippet: DXS253E facilitates CRC cell malignant phenotype and aerobic glycolysis via the AKT/mTOR pathway. A Western blot assay of DXS253E protein expression levels in NCM460, LoVo, HCT116, RKO, SW480, and SW620 cell lines. B qRT-PCR assay of DXS253E mRNA expression levels in normal epithelial colon cell line NCM460 and CRC cell lines. C DXS253E overexpression accelerates the proliferation of RKO and HCT116 cells. D High levels of DXS253E expression enhance colony formation in CRC cells. E Overexpression of DXS253E enhances the malignant progression of RKO and HCT116 cells. Bar graphs show the number of migrated or invaded cells. F High levels of DXS253E decrease the generation of reactive oxygen species (ROS). G DXS253E overexpression elevates lactate production. H, I DXS253E regulates the level of glycolytic genes in CRC cells, including HK2, PKM2, GLUT1, and LDHA with qRT-PCR and western blot. J DXS253E overexpression in CRC cells mediates the activation of the AKT/mTOR pathway. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: After heat-induced epitope retrieval, the slides were incubated with DXS253E antibody (1:100, Cat #19,909-1-AP, Proteintech) overnight at 4 °C, and subsequently exposed to an anti-rabbit antibody for 40 min and stained with fresh 3,3′-Diaminobenzidine substrate within a controlled reaction time of 1–2 min. Next, sections were counterstained with hematoxylin, rinsed to blue, dehydrated, and sealed.

Techniques: Western Blot, Expressing, Quantitative RT-PCR, Over Expression, Activation Assay