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Image Search Results
Journal: International journal of molecular sciences
Article Title: Isorhapontigenin Inhibits Cell Growth, Angiogenesis, Migration, and Invasion of Non-Small-Cell Lung Cancer Cells Through NEDD9 Signaling.
doi: 10.3390/ijms26094207
Figure Lengend Snippet: Figure 6. ISO suppressed migration and invasion through NEDD9/β-Catenin signaling. (A) A549 cells were treated with 40 µM ISO for 24 h. The whole-cell lysates were collected for immunoblotting analysis. (B) A549 cells with and without stable expression of NEDD9 treated with 40 µM ISO for 24 h were subjected to immunoblotting analysis. The band intensity was evaluated using ImageJ (version 1.53m), followed by normalization. (C,D) A549 cells with and without stable expression of NEDD9 in combination with ISO treatment (40 µM) were subjected to a transwell invasion and migration assay. After 48 h, cells were fixed and stained. Images were captured using an Olympus microscope. Invaded (C) and migrated cells (D) were counted and recorded. * and #, p < 0.05, compared to the cells without stable expression of NEDD9 and the cells with stable expression of NEDD9 but without ISO treatment, respectively.
Article Snippet: Primary antibodies against NEDD9 (Cat# 4044),
Techniques: Migration, Western Blot, Expressing, Staining, Microscopy