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    Thermo Fisher anti rad21
    Recruitment of architectural proteins and topological changes upon integration of the TgN(38-40) construct. (A) ChIP of CTCF and <t>RAD21</t> in wild-type or TgN(38-40) E12.5 whole limbs. The window displayed corresponds to the native region CS38-40. Peak calling is represented as black boxes. Bottom, extension of both the TgN(38-40) construct and del(CS38-40) background. (B) Hi-C showing the host locus of chromosome 10 in wild-type whole limbs at E12.5. Below the Hi-C heatmap, wild-type CTCFs (red or blue arrowheads) and topological domains (horizontal bars). (C) Distribution of H3K27ac (green) over the host locus in the distal part of wild-type E12.5 forelimbs. (D) Top, 4C-seq using CS38 and CS40 as viewpoints in TgN(38-40) E12.5 limbs. Bottom, 4C-seq tracks of CTCF-left and CTCF-right viewpoints in both TgN(38-40) homozygous (red lines) and wild-type samples (blue lines). Percentages of 4C-seq contact changes beyond the integration site are shown. Black arrowheads indicate 4C-seq viewpoints. (E) ChIP of CTCF and RAD21 over the host landscape in TgN(38-40) .
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    Recruitment of architectural proteins and topological changes upon integration of the TgN(38-40) construct. (A) ChIP of CTCF and RAD21 in wild-type or TgN(38-40) E12.5 whole limbs. The window displayed corresponds to the native region CS38-40. Peak calling is represented as black boxes. Bottom, extension of both the TgN(38-40) construct and del(CS38-40) background. (B) Hi-C showing the host locus of chromosome 10 in wild-type whole limbs at E12.5. Below the Hi-C heatmap, wild-type CTCFs (red or blue arrowheads) and topological domains (horizontal bars). (C) Distribution of H3K27ac (green) over the host locus in the distal part of wild-type E12.5 forelimbs. (D) Top, 4C-seq using CS38 and CS40 as viewpoints in TgN(38-40) E12.5 limbs. Bottom, 4C-seq tracks of CTCF-left and CTCF-right viewpoints in both TgN(38-40) homozygous (red lines) and wild-type samples (blue lines). Percentages of 4C-seq contact changes beyond the integration site are shown. Black arrowheads indicate 4C-seq viewpoints. (E) ChIP of CTCF and RAD21 over the host landscape in TgN(38-40) .

    Journal: bioRxiv

    Article Title: CONTEXT-INDEPENDENT FUNCTION OF A CHROMATIN BOUNDARY IN VIVO

    doi: 10.1101/2021.02.11.430727

    Figure Lengend Snippet: Recruitment of architectural proteins and topological changes upon integration of the TgN(38-40) construct. (A) ChIP of CTCF and RAD21 in wild-type or TgN(38-40) E12.5 whole limbs. The window displayed corresponds to the native region CS38-40. Peak calling is represented as black boxes. Bottom, extension of both the TgN(38-40) construct and del(CS38-40) background. (B) Hi-C showing the host locus of chromosome 10 in wild-type whole limbs at E12.5. Below the Hi-C heatmap, wild-type CTCFs (red or blue arrowheads) and topological domains (horizontal bars). (C) Distribution of H3K27ac (green) over the host locus in the distal part of wild-type E12.5 forelimbs. (D) Top, 4C-seq using CS38 and CS40 as viewpoints in TgN(38-40) E12.5 limbs. Bottom, 4C-seq tracks of CTCF-left and CTCF-right viewpoints in both TgN(38-40) homozygous (red lines) and wild-type samples (blue lines). Percentages of 4C-seq contact changes beyond the integration site are shown. Black arrowheads indicate 4C-seq viewpoints. (E) ChIP of CTCF and RAD21 over the host landscape in TgN(38-40) .

    Article Snippet: Antibodies (CTCF, Active Motif 61311 or RAD21, Abcam ab992) were incubated with Dynabeads Protein A (Thermo Fisher Scientific, 10001D) for 3 hours on a rotating wheel at 4°C.

    Techniques: Construct, Chromatin Immunoprecipitation, Hi-C

    Recruitment of architectural proteins and topological changes upon integration of the TgN(38–40) construct. (A) ChIP of CTCF and RAD21 in wild-type or TgN(38–40) E12.5 whole limbs. The window displayed corresponds to the native region CS38-40. Dashed lines are displayed for better comparison between the occupancy of various CTCF sites. Peak calling is represented as black boxes. Bottom, extension of both the TgN(38–40) construct and del(CS38-40) background. (B) Hi-C showing the host locus of chromosome 10 in wild-type whole limbs at E12.5. Below the Hi-C heatmap, wild-type CTCFs (red or blue arrowheads) and topological domains (horizontal bars). (C) Distribution of H3K27ac (green) over the host locus in the distal part of wild-type E12.5 forelimbs. (D) Top, 4C-seq using CS38 and CS40 as viewpoints in TgN(38–40) E12.5 limbs. Bottom, 4C-seq tracks of CTCF-left and CTCF-right viewpoints in both TgN(38–40) homozygous (red lines) and wild-type samples (blue lines). Percentages of 4C-seq contact changes beyond the integration site are shown. Black arrowheads indicate 4C-seq viewpoints. (E) ChIP of CTCF and RAD21 over the host landscape in TgN(38–40) .

    Journal: PLoS Genetics

    Article Title: Induction of a chromatin boundary in vivo upon insertion of a TAD border

    doi: 10.1371/journal.pgen.1009691

    Figure Lengend Snippet: Recruitment of architectural proteins and topological changes upon integration of the TgN(38–40) construct. (A) ChIP of CTCF and RAD21 in wild-type or TgN(38–40) E12.5 whole limbs. The window displayed corresponds to the native region CS38-40. Dashed lines are displayed for better comparison between the occupancy of various CTCF sites. Peak calling is represented as black boxes. Bottom, extension of both the TgN(38–40) construct and del(CS38-40) background. (B) Hi-C showing the host locus of chromosome 10 in wild-type whole limbs at E12.5. Below the Hi-C heatmap, wild-type CTCFs (red or blue arrowheads) and topological domains (horizontal bars). (C) Distribution of H3K27ac (green) over the host locus in the distal part of wild-type E12.5 forelimbs. (D) Top, 4C-seq using CS38 and CS40 as viewpoints in TgN(38–40) E12.5 limbs. Bottom, 4C-seq tracks of CTCF-left and CTCF-right viewpoints in both TgN(38–40) homozygous (red lines) and wild-type samples (blue lines). Percentages of 4C-seq contact changes beyond the integration site are shown. Black arrowheads indicate 4C-seq viewpoints. (E) ChIP of CTCF and RAD21 over the host landscape in TgN(38–40) .

    Article Snippet: Antibodies (CTCF, Active Motif 61311 or RAD21, Abcam ab992) were incubated with Dynabeads Protein A (Thermo Fisher Scientific, 10001D) for 3 hours on a rotating wheel at 4°C.

    Techniques: Construct, Chromatin Immunoprecipitation, Hi-C