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Image Search Results
Journal: bioRxiv
Article Title: In vivo single-cell CRISPR screening for microproteins identifies a critical ribosomal component
doi: 10.1101/2025.03.17.643322
Figure Lengend Snippet: (A) Western blot showing four microproteins tagged with Turbo GFP (TuGFP) in both wild-type keratinocytes and Hek293T cells. The following microproteins were tagged: Gm7239::TuGFP (45.1kDa), Gm5586::TuGFP (44.3kDa), Gm8420::TuGFP (31.2 kDa) and Gm10076::TuGFP (30.1 kDa). Lower panel shows the 45 kDa actin protein as loading control. (B) Immunofluorescence analyses of subcellular localization for the 31 amino acid Gm13935 microprotein tagged with four different Turbo GFP constructs: Gm13935::TuGFP, Gm13935::P2A::TuGFP, Gm13935::V5::P2A::TuGFP and TuGFP::V5::Gm13935 . (C) Western blot showing expression of C-terminally tagged Gm13935::TuGFP keratinocytes sorted sorted once or three times, alongside N-terminally tagged TuGFP::V5::Gm13935 treated with the proteasome inhibitor MG132 (2 µM and 10 µM) for 2 hours. (D) Western blot showing a time-course analysis of C-terminally tagged Gm13935::TuGFP and N-terminally tagged TuGFP::V5::Gm13935 keratinocytes treated with proteosome inhibitor MG132 (2µM) for 2, 4 and 8 hours.
Article Snippet: The puromycin resistance cassette in the original CROP-seq-Guide-Puro vector (Addgene #86708) was replaced with a CRE sequence, amplified from
Techniques: Western Blot, Control, Immunofluorescence, Construct, Expressing