Journal: BMC Microbiology

Article Title: Membrane changes during syntrophic interactions of an archaeal-bacterial coculture

doi: 10.1186/s12866-025-04509-z

Figure Lengend Snippet: Scheme of experimental set up and visualization of the Methanococcus maripaludis ( M. maripaludis ; archaeon) and Desulfovibrio vulgaris ( D. vulgaris ; bacterium) cocultures. A Monocultures of both wild type (WT) species and cocultures obtained from different generations during adaptation to syntrophic growth have been analyzed for their lipids, proteome and transcriptome (green). From all three cocultures, the differentially adapted strains of both species were isolated into monocultures and checked for occurred mutations (orange). Monocultures of WT and isolated strains were used to start initial cocultures using different combinations and analyzed for their lipids after grown once to stationary phase (blue). B Microscopy image of M. maripaludis (cocci, blue) and D. vulgaris (vibrio shaped) in aggregates of Anc-Co, 300-Co and 1000-Co cocultures during early stationary phase. WT: wild type monoculture, Mmp: M. maripaludis , Dvu: D. vulgaris , Anc-Co: non-evolved/ancestral coculture, 300-Co: evolved 300 generations coculture, 1000-Co: evolved 1,000 generations coculture

Article Snippet: M. maripaludis S2 (DSM14266) and D. vulgaris Hildenborough (DSM644) were obtained from DSMZ culture collection.

Techniques: Isolation, Microscopy

Journal: Annals of Hematology

Article Title: The BET bromodomain inhibitor ZEN-3365 targets the Hedgehog signaling pathway in acute myeloid leukemia

doi: 10.1007/s00277-021-04602-z

Figure Lengend Snippet: BET inhibitor ZEN-3365 reduces GLI reporter activity in vitro. a Western blot of GLI protein levels under ZEN-3365 treatment. MV4-11, MOLM13, and OCI-AML3 were cultivated for 3 days with 100 or 200 nM ZEN-3365. b GLI promoter assays reveal the dependence of GLI expression and BRD4 regulation. MV4-11, HL-60, Kasumi-1, and OCI-AML3 were treated with different concentrations of ZEN-3365 (25 to 2500 nM) for 24 h. c Time course experiment for BRD4 inhibition with 25, 50, 250, 1000, and 2500 nM ZEN-3365 on MV4-11 cells reveals a stable inhibitory function over 4 days

Article Snippet: The following human AML cell lines were used: MV4-11 (ATCC, #CRL9591, RRID:CVCL_0064), MOLM13 (DSMZ, #ACC554, RRID:CVCL_2119), HL60 (DSMZ, #ACC3, RRID:CVCL_002), KG-1 (DSMZ, #ACC14, RRID:CVCL_0374), Kasumi-1 (DSMZ, #ACC220, RRID_CVCL_0589), OCI-AML3 (DSMZ, #ACC582, RRID:CVCL_1844).

Techniques: Activity Assay, In Vitro, Western Blot, Expressing, Inhibition

Journal: Annals of Hematology

Article Title: The BET bromodomain inhibitor ZEN-3365 targets the Hedgehog signaling pathway in acute myeloid leukemia

doi: 10.1007/s00277-021-04602-z

Figure Lengend Snippet: Combinatorial treatment of AML reporter cell lines with ZEN-3365 and GANT-61 decreases GLI reporter activity in vitro. a MV4-11, b MOLM13, c Kasumi-1, and d HL60 reporter cell lines were treated with 2500 or 5000 nM GANT-61 and 50 nM or 100 nM ZEN-3365 for 24 h. Significance calculated against GANT-61 single treatment, * p < 0.05, ** p < 0.01, and ns = not significant

Article Snippet: The following human AML cell lines were used: MV4-11 (ATCC, #CRL9591, RRID:CVCL_0064), MOLM13 (DSMZ, #ACC554, RRID:CVCL_2119), HL60 (DSMZ, #ACC3, RRID:CVCL_002), KG-1 (DSMZ, #ACC14, RRID:CVCL_0374), Kasumi-1 (DSMZ, #ACC220, RRID_CVCL_0589), OCI-AML3 (DSMZ, #ACC582, RRID:CVCL_1844).

Techniques: Activity Assay, In Vitro

Journal: Annals of Hematology

Article Title: The BET bromodomain inhibitor ZEN-3365 targets the Hedgehog signaling pathway in acute myeloid leukemia

doi: 10.1007/s00277-021-04602-z

Figure Lengend Snippet: ZEN-3365 and GANT-61 present antiproliferative effects. GANT-61 (5 or 10 μM) and ZEN-3365 (100 or 200 nM) inhibit cell proliferation over 7 days for a MV4-11, b HL60, c KG-1, d MOLM13, e Kasumi-1, and f OCI-AML3. g Combinatorial treatment of primary AML sample with GANT-61 (5 or 10 M) and ZEN-3365 (100 or 200 nM) leads to a decreased cell proliferation after 4 days. Significance calculated against GANT-61 treatment alone, * p <0.05, ** p <0.01, and ns=not significant

Article Snippet: The following human AML cell lines were used: MV4-11 (ATCC, #CRL9591, RRID:CVCL_0064), MOLM13 (DSMZ, #ACC554, RRID:CVCL_2119), HL60 (DSMZ, #ACC3, RRID:CVCL_002), KG-1 (DSMZ, #ACC14, RRID:CVCL_0374), Kasumi-1 (DSMZ, #ACC220, RRID_CVCL_0589), OCI-AML3 (DSMZ, #ACC582, RRID:CVCL_1844).

Techniques:

Journal: Annals of Hematology

Article Title: The BET bromodomain inhibitor ZEN-3365 targets the Hedgehog signaling pathway in acute myeloid leukemia

doi: 10.1007/s00277-021-04602-z

Figure Lengend Snippet: The colony formation potential of AML cell lines is decreased under single or combinatorial treatment. a MV4-11, b MOLM13, c OCI-AML3, and d HL60 were treated with 5 μM GANT-61 and 0.1 μM ZEN-3365 or in combination and cultured in methocult medium for one week. Colony numbers were counted with an inverted microscope. * p <0.05, ** p <0.01, and ns=not significant

Article Snippet: The following human AML cell lines were used: MV4-11 (ATCC, #CRL9591, RRID:CVCL_0064), MOLM13 (DSMZ, #ACC554, RRID:CVCL_2119), HL60 (DSMZ, #ACC3, RRID:CVCL_002), KG-1 (DSMZ, #ACC14, RRID:CVCL_0374), Kasumi-1 (DSMZ, #ACC220, RRID_CVCL_0589), OCI-AML3 (DSMZ, #ACC582, RRID:CVCL_1844).

Techniques: Cell Culture, Inverted Microscopy