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Image Search Results
Journal:
Article Title: Onset of natural killer cell lymphomas in transgenic mice carrying a truncated HMGI-C gene by the chronic stimulation of the IL-2 and IL-15 pathway
doi: 10.1073/pnas.141224998
Figure Lengend Snippet: Overexpression of IL-2 and IL-15 and their receptors in NK-T/NK cells derived from HMGI-C/T TG mice. (A) Expression of IL-2 Rα, IL-2, IL-15, and IL-15 Rα proteins in two different control spleens and in six different pathological specimens. The same Western blot was incubated with antibody vs. actin protein to normalize the amount of loaded proteins. (B) RT-PCR analysis of RNA extracted from two different WT spleens and six pathological specimens. The expression of IL-15, IL-15 Rα, IL-2, and IL-2 Rα is shown. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression was determined as an internal control of RNA quantity and status. (C) IL-15 expression analyzed by immunohistochemistry in two different pathological lymph nodes.
Article Snippet: The primary antibodies against IL-15, IL-2,
Techniques: Over Expression, Derivative Assay, Expressing, Western Blot, Incubation, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry
Journal:
Article Title: Onset of natural killer cell lymphomas in transgenic mice carrying a truncated HMGI-C gene by the chronic stimulation of the IL-2 and IL-15 pathway
doi: 10.1073/pnas.141224998
Figure Lengend Snippet: HMGI-C/T protein binds to and activates the IL-15 promoter. (A) IL-15 −84 to −52 oligonucleotide recognized by increasing amounts (5, 10, 20, 50, and 100 ng, respectively) of HMGI/Y (lanes 2–6) or HMGI/C recombinant protein (lanes 8–11). (B) In vitro binding of HMGI-C protein to the IL-15 oligonucleotide competed with 30 ng of HMGI/C recombinant protein alone (lane 2) or in presence of two unrelated oligonucleotides (lanes 3 and 4). Specific competition of DNA protein interaction was obtained with a 50- to 100-fold excess of IL-2 Rα (lane 5), IL-2 (lane 6), or IL-15 (lanes 7 and 8) cold oligonucleotides. (C) Luciferase assay using the IL-15 promoter region transfected alone or with increasing concentrations of pCMV-HMGI-C/T vector. (D) Luciferase assay using the IL-15 promoter region transfected alone or with pCMV-NF-κB alone or in combination with pCMV-HMGI-C/T vector. In all cases, a typical experiment is reported.
Article Snippet: The primary antibodies against IL-15, IL-2,
Techniques: Recombinant, In Vitro, Binding Assay, Luciferase, Transfection, Plasmid Preparation