18477 Search Results


influenzae gra m bact eria  (ATCC)
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ATCC influenzae gra m bact eria
Influenzae Gra M Bact Eria, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l threonine t butyl ester  (Chem Impex International)
95
Chem Impex International l threonine t butyl ester
L Threonine T Butyl Ester, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcag h2b gfp plasmid  (Addgene inc)
93
Addgene inc pcag h2b gfp plasmid
Pcag H2b Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glo1  (Proteintech)
93
Proteintech glo1
(A) Western blot analysis of the expression of senescence-related genes in V6.5 cells treated with various concentration gradients of MG. ACTIN served as the loading control. (B) Western blot analysis of the expression of senescence-related genes in reprogramming cells treated with MG. ACTIN served as the loading control. (C) Reprogramming cells were treated with MG or control, and the SA-β-gal-positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. Scale bars, 100 μm. (D) MG was added to the medium of MEF cells starting from 2 d after infection with virus expressing the four factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. (E) Western blot analysis of the expression of senescence-related genes and argpyrimidine in V6.5 cells stably expressing shGldc or the NTC. ACTIN served as the loading control. (F) Western blot analysis of the expression of senescence-related genes and argpyrimidine in reprogramming cells expressing shGldc the NTC at various time points. ACTIN served as the loading control. (G) Reprogramming cells were infected with viruses expressing shGldc or the NTC, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the indicated group; t test. Scale bars, 100 μm. (H) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing <t>pSIN-3×flag-Glo1</t> or were supplemented with 1 mM carnosine, followed by qRT-PCR analysis of senescence-related genes. The data were presented as the mean ± SD of three independent experiments. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (I) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (J) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (K) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. Scale bars, 100 μm. (L) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (M) MEFs were induced with the indicated factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. NS, not significant. Source data are available for this figure.
Glo1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glo1/product/Proteintech
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sbi-0206955 (cat. no.: 18477)  (Cayman Chemical)
90
Cayman Chemical sbi-0206955 (cat. no.: 18477)
(A) Western blot analysis of the expression of senescence-related genes in V6.5 cells treated with various concentration gradients of MG. ACTIN served as the loading control. (B) Western blot analysis of the expression of senescence-related genes in reprogramming cells treated with MG. ACTIN served as the loading control. (C) Reprogramming cells were treated with MG or control, and the SA-β-gal-positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. Scale bars, 100 μm. (D) MG was added to the medium of MEF cells starting from 2 d after infection with virus expressing the four factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. (E) Western blot analysis of the expression of senescence-related genes and argpyrimidine in V6.5 cells stably expressing shGldc or the NTC. ACTIN served as the loading control. (F) Western blot analysis of the expression of senescence-related genes and argpyrimidine in reprogramming cells expressing shGldc the NTC at various time points. ACTIN served as the loading control. (G) Reprogramming cells were infected with viruses expressing shGldc or the NTC, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the indicated group; t test. Scale bars, 100 μm. (H) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing <t>pSIN-3×flag-Glo1</t> or were supplemented with 1 mM carnosine, followed by qRT-PCR analysis of senescence-related genes. The data were presented as the mean ± SD of three independent experiments. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (I) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (J) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (K) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. Scale bars, 100 μm. (L) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (M) MEFs were induced with the indicated factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. NS, not significant. Source data are available for this figure.
Sbi 0206955 (Cat. No.: 18477), supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sbi-0206955 (cat. no.: 18477)/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
sbi-0206955 (cat. no.: 18477) - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


(A) Western blot analysis of the expression of senescence-related genes in V6.5 cells treated with various concentration gradients of MG. ACTIN served as the loading control. (B) Western blot analysis of the expression of senescence-related genes in reprogramming cells treated with MG. ACTIN served as the loading control. (C) Reprogramming cells were treated with MG or control, and the SA-β-gal-positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. Scale bars, 100 μm. (D) MG was added to the medium of MEF cells starting from 2 d after infection with virus expressing the four factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. (E) Western blot analysis of the expression of senescence-related genes and argpyrimidine in V6.5 cells stably expressing shGldc or the NTC. ACTIN served as the loading control. (F) Western blot analysis of the expression of senescence-related genes and argpyrimidine in reprogramming cells expressing shGldc the NTC at various time points. ACTIN served as the loading control. (G) Reprogramming cells were infected with viruses expressing shGldc or the NTC, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the indicated group; t test. Scale bars, 100 μm. (H) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or were supplemented with 1 mM carnosine, followed by qRT-PCR analysis of senescence-related genes. The data were presented as the mean ± SD of three independent experiments. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (I) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (J) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (K) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. Scale bars, 100 μm. (L) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (M) MEFs were induced with the indicated factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. NS, not significant. Source data are available for this figure.

Journal: Life Science Alliance

Article Title: Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications

doi: 10.26508/lsa.201900413

Figure Lengend Snippet: (A) Western blot analysis of the expression of senescence-related genes in V6.5 cells treated with various concentration gradients of MG. ACTIN served as the loading control. (B) Western blot analysis of the expression of senescence-related genes in reprogramming cells treated with MG. ACTIN served as the loading control. (C) Reprogramming cells were treated with MG or control, and the SA-β-gal-positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. Scale bars, 100 μm. (D) MG was added to the medium of MEF cells starting from 2 d after infection with virus expressing the four factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with CTR; t test. (E) Western blot analysis of the expression of senescence-related genes and argpyrimidine in V6.5 cells stably expressing shGldc or the NTC. ACTIN served as the loading control. (F) Western blot analysis of the expression of senescence-related genes and argpyrimidine in reprogramming cells expressing shGldc the NTC at various time points. ACTIN served as the loading control. (G) Reprogramming cells were infected with viruses expressing shGldc or the NTC, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the indicated group; t test. Scale bars, 100 μm. (H) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or were supplemented with 1 mM carnosine, followed by qRT-PCR analysis of senescence-related genes. The data were presented as the mean ± SD of three independent experiments. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (I) V6.5 cells stably expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (J) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, followed by Western blot analysis of senescence-related genes and argpyrimidine modification. ACTIN served as the loading control. (K) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1 or EV, and SA-β-gal–positive cells were counted. The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. Scale bars, 100 μm. (L) Reprogramming cells expressing shGldc or the NTC were further infected with viruses expressing pSIN-3×flag-Glo1. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. (M) MEFs were induced with the indicated factors. AP staining (upper panel) showed the iPSC colonies formed. The AP- and Ssea1-positive iPSC colonies were counted (lower panel). The data were presented as the mean ± SD. * P < 0.05 compared with the NTC, # P < 0.05 compared with the Gldc knockdown group; t test. NS, not significant. Source data are available for this figure.

Article Snippet: Primary antibodies against the following proteins were used: OCT4 (from Stemgent); SOX2 (from Millipore); MYC (from Epitomics); KLF4, SSEA1, SHMT1, SHMT2, TDH, AMT, H3, P15, and MG (from Abcam); H3K4me3, H3K9me3, H3K27me3, and H3K36me3 (from Cell Signaling Technology); FLAG (from Sigma-Aldrich); ACTIN, phosphoglycerate dehydrogenase, PSAT1, PSPH, GCAT, GLDC, GCSH, P16, P21, and GLO1 (from Proteintech).

Techniques: Western Blot, Expressing, Concentration Assay, Control, Infection, Virus, Staining, Stable Transfection, Quantitative RT-PCR, Knockdown, Modification