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Image Search Results
Journal: Cancer biology & therapy
Article Title: BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40.
doi: 10.1080/15384047.2024.2375440
Figure Lengend Snippet: Figure 7. BRG1 induces the expession of TOMM40 in HCC cells. (a) HepG2 cells were stained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. (b and c) the percentage of positive cells was analyzed by ImageJ software (n = 10). **p < .01, ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC. (d) HuH-7 cells were stained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. (e and f) the percentage of positive cells was analyzed by ImageJ software (n = 10). ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC.
Article Snippet: After blocking with 5% nonfat milk for 1 h at room temperature, the membranes were incubated with indicated primary antibodies: anti-rabbit BRG1 antibody (1:1000, 21634–1-AP, Proteintech, China),
Techniques: Staining, Knockdown, Over Expression, Software
Journal: Cancer biology & therapy
Article Title: BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40.
doi: 10.1080/15384047.2024.2375440
Figure Lengend Snippet: Figure 8. BRG1 induces the expession of TOMM40 in HCC cells. (a) HepG2 cells were immunostained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. Scale bar, 20 μm. (b and c) the relative fluorescence intensity was analyzed by ImageJ software (n = 10). ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC. (d) HuH-7 cells were immunostained with TOMM40 and BRG1 antibody after BRG1 knockdown or overexpression. Scale bar, 20 μm. (e and f) the relative fluorescence intensity was analyzed by Image J software (n = 10). ***p < .001 vs siRNA-NC, ###p < .001 vs pcDNA3.1-NC.
Article Snippet: After blocking with 5% nonfat milk for 1 h at room temperature, the membranes were incubated with indicated primary antibodies: anti-rabbit BRG1 antibody (1:1000, 21634–1-AP, Proteintech, China),
Techniques: Knockdown, Over Expression, Fluorescence, Software
Journal: Cancer biology & therapy
Article Title: BRG1 promotes liver cancer cell proliferation and metastasis by enhancing mitochondrial function and ATP5A1 synthesis through TOMM40.
doi: 10.1080/15384047.2024.2375440
Figure Lengend Snippet: Figure 9. BRG1 activates TOMM40/ATP5A1 pathway in HCC cells. (a) The expressions of BRG1, TOMM40 and ATP5A1 were analyzed using Western blotting after BRG1 knockdown or overexpression in HepG2 cells. (b-d) the relative intensities of BRG1, TOMM40 and ATP5A1 were determined using ImageJ software (n = 3). **p < .01, ***p < .001 vs siRNA-NC, ##p < .01, ###p < .001 vs pcDNA3.1-NC. (e) The expressions of BRG1, TOMM40 and ATP5A1 were analyzed using Western blotting after BRG1 knockdown or overexpression in HuH-7 cells. (f-h) the relative intensity were determined using ImageJ software (n = 3). **p < .01, ***p < .001 vs siRNA-NC, ###p < .001 vs pcDNA3.1-NC. (i-l) the mRNA expressions level of BRG1, TOMM40 and ATP5A1 were analyzed using RT-qPCR.(n = 3). ##p < .01, ###p < .001 vs siRNA-NC, ***p < .001 vs pcDNA3.1-NC.
Article Snippet: After blocking with 5% nonfat milk for 1 h at room temperature, the membranes were incubated with indicated primary antibodies: anti-rabbit BRG1 antibody (1:1000, 21634–1-AP, Proteintech, China),
Techniques: Western Blot, Knockdown, Over Expression, Software, Quantitative RT-PCR