Journal: Analytical Chemistry

Article Title: Aptamer Sandwich Lateral Flow Assay (AptaFlow) for Antibody-Free SARS-CoV-2 Detection

doi: 10.1021/acs.analchem.2c00554

Figure Lengend Snippet: (A) Step-by-step process for AptaFlow. (i) incubating reagents with SARS-CoV-2 sample. (ii) dipping of the test strip into the sample. (iii) washing to reduce nonspecific signals. (iv) enhancing signal to increase sensitivity. (B) Limit of detection of LFA system with UV-inactivated virus (UV) at various concentrations (copies/mL) and heat-inactivated (HI) virus (negative control). Bars indicate mean. Brackets indicate 95% CI. Statistical significance was determined by one-way ANOVA with Dunnett correction. * indicates p < 0.05 and **** indicates p < 0.0001 when compared to HI 1 × 10 7 copies/mL; n = 3–4 per experimental group. A representative image is shown.

Article Snippet: Expression was monitored with SARS-CoV-2 Spike Human anti-SARS-CoV-2, Alexa Fluor 488 (Novus Biologicals cat. no. NBP290980AF488) by flow cytometry.

Techniques: Stripping Membranes, Virus, Negative Control

Journal: Investigative ophthalmology & visual science

Article Title: Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway.

doi: 10.1167/iovs.18-25572

Figure Lengend Snippet: FIGURE 1. SSRI treatment is closely related to depression-associated dry eye disease and causes increased tear serotonin levels with a serious inflammatory response and cell apoptosis at the ocular surface. (A) The clinical indicators based on the baseline data and data collected after 90 days of treatment in the SSRI and control groups. The P value means the difference between ‘‘change’’ in SSRI and control groups. (B) ELISA analysis showing the tear serotonin levels of the patients with SSRI treatment and the control group. (C, D) Protein chip analysis showing the inflammatory cytokine content following SSRI treatment, based on examination of 507 proteins, including TLR4, IL-6, and IL-10. (E) qRT-PCR analysis showing the conjunctival TLR2 and TLR4 and inflammatory cytokine IL1b, IL10, and TNFa mRNA levels between the patients with SSRI treatment and the control group. (F) qRT-PCR analysis showing the conjunctival mRNA levels of the proapoptotic genes AIF, BAD, and BAX and the antiapoptotic genes BCL2 and XIAP between the patients with SSRI treatment and the control group. *P < 0.05, **P < 0.01.

Article Snippet: The membranes were incubated overnight at 48C with primary antibodies, including IL-1b (1:1000; Abcam), IL-6 (1:1000; Proteintech, Wuhan, China), IL-10 (1:500; Abcam), TNF-a (1:500; Abcam), TLR2 (1:1000; Abcam), TLR4 (1:200; Santa Cruz Biotechnology), AIF (1:1000; Proteintech), BAD (1:1000; Abcam), BAX (1:1000; Proteintech), P-p65 (1:2000; Abcam), p65 (1:2000; Abcam), IjBa (1:1000; Abcam), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:12000; Proteintech).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Investigative ophthalmology & visual science

Article Title: Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway.

doi: 10.1167/iovs.18-25572

Figure Lengend Snippet: FIGURE 4. Serotonin can induce corneal epithelial cell inflammation and apoptosis. (A) qRT-PCR analysis showing the mRNA levels of the inflammatory cytokines TLR4, TLR2, IL1b, IL6, IL10, and TNFa with the serotonin level. (B, C) Western blot analysis showing the protein levels in the growth plate of TLR4, TLR2, IL-1b, IL-6, IL-10, and TNF-a with the serotonin level. (D) ELISA analysis of the related IL-1b, IL-10, and TNF-a content in the cell supernatants in cells with increased serotonin levels. (E, F) Western blot analysis showing the protein levels in the growth plate of the proapoptotic proteins BAX, BAD, and AIF with the serotonin level. (E) Flow cytometry showing apoptosis in corneal epithelial cells with serotonin treatment. *P < 0.05, **P < 0.01.

Article Snippet: The membranes were incubated overnight at 48C with primary antibodies, including IL-1b (1:1000; Abcam), IL-6 (1:1000; Proteintech, Wuhan, China), IL-10 (1:500; Abcam), TNF-a (1:500; Abcam), TLR2 (1:1000; Abcam), TLR4 (1:200; Santa Cruz Biotechnology), AIF (1:1000; Proteintech), BAD (1:1000; Abcam), BAX (1:1000; Proteintech), P-p65 (1:2000; Abcam), p65 (1:2000; Abcam), IjBa (1:1000; Abcam), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:12000; Proteintech).

Techniques: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Investigative ophthalmology & visual science

Article Title: Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway.

doi: 10.1167/iovs.18-25572

Figure Lengend Snippet: FIGURE 5. Serotonin receptors are indispensable for serotonin-induced cell inflammation and apoptosis in corneal epithelial cells. (A) qRT-PCR analysis showing the HTR1A, 1B, 1D, 1E, 1F, 2A, 2B, 2C, 3A, 3B, 4, 5A, 6, and 7 mRNA levels in corneal epithelial cells. (B, C) Western blot assay showing the expression levels of the proinflammatory response element TLR4, inflammatory cytokines IL-6 and IL-10, and proapoptotic proteins BAX, BAD, and AIF in asenapine maleate–treated corneal epithelial cells. (D) ELISA analysis of the related IL-1b, IL-10, and TNF-a content in the supernatants of cells with accumulated serotonin treated with asenapine maleate. (G) Flow cytometry showing apoptosis in corneal epithelial cells with serotonin treated with asenapine maleate. *P < 0.05, **P < 0.01.

Article Snippet: The membranes were incubated overnight at 48C with primary antibodies, including IL-1b (1:1000; Abcam), IL-6 (1:1000; Proteintech, Wuhan, China), IL-10 (1:500; Abcam), TNF-a (1:500; Abcam), TLR2 (1:1000; Abcam), TLR4 (1:200; Santa Cruz Biotechnology), AIF (1:1000; Proteintech), BAD (1:1000; Abcam), BAX (1:1000; Proteintech), P-p65 (1:2000; Abcam), p65 (1:2000; Abcam), IjBa (1:1000; Abcam), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:12000; Proteintech).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Investigative ophthalmology & visual science

Article Title: Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway.

doi: 10.1167/iovs.18-25572

Figure Lengend Snippet: FIGURE 6. Serotonin induces cell inflammation and apoptosis by activating the NF-jB pathway. (A, B) Western blot analysis showing the p65 phosphorylation level and the IjBa protein level with the serotonin level. (C, D) Western blot analysis showing the P-p65, IjBa, proinflammatory response element TLR4, inflammatory cytokine IL-1b, and proapoptotic protein BAX, BAD, and AIF protein levels in JSH-23–treated corneal epithelial cells. (E) ELISA analysis of the related IL-1b, IL-10, and TNF-a content in the supernatants of cells treated with the NF-jB signaling inhibitor. (F) Flow cytometry showing apoptosis in corneal epithelial cells with accumulated serotonin following JSH-23 treatment. *P < 0.05, **P < 0.01.

Article Snippet: The membranes were incubated overnight at 48C with primary antibodies, including IL-1b (1:1000; Abcam), IL-6 (1:1000; Proteintech, Wuhan, China), IL-10 (1:500; Abcam), TNF-a (1:500; Abcam), TLR2 (1:1000; Abcam), TLR4 (1:200; Santa Cruz Biotechnology), AIF (1:1000; Proteintech), BAD (1:1000; Abcam), BAX (1:1000; Proteintech), P-p65 (1:2000; Abcam), p65 (1:2000; Abcam), IjBa (1:1000; Abcam), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:12000; Proteintech).

Techniques: Western Blot, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Biochimica et biophysica acta

Article Title: Important roles for membrane lipids in haloarchaeal bioenergetics.

doi: 10.1016/j.bbamem.2016.08.010

Figure Lengend Snippet: Figure 2: Lipidomics of haloarchaea. Relative abundance of major lipid classes (glycerolipids, menaquinones, carotenoids and squalenes) in Hfx. volcanii, Hlb. salinarum, Hrr. lacusprofundi, Hrr. sodomense and Hpn. natans. The generation times for (*), (**) and (***) are reported in [57], [27] and [42], respectively. A more detailed contribution of only glycerolipids is given in (B), where for all five species, the anionic lipids (sum of PG, Me-PGP, SDG and CL) clearly dominated the total polar lipid fraction. Note that Hrr. sodomense and Hpn. natans were each analyzed only in a singlet analysis. Relative percentages in tabular form are provided in Table S2. Compound abbreviations are presented in Fig. S2; n.d., not determined.

Article Snippet: [°C] O2 [%] pH Hfx. volcanii (ATCC 29605) ATCC 974 Yes 2 250 37 21 7 Hrr. lacusprofundi (ATCC 49238) ATCC 974 Yes 2 250 37 21 7 Hbt. salinarum (DSM 617) [41] No 4 81 37 21 7 Hrr. sodomense (DSM 3755)* DSMZ 372 No [42] 3.4 81 37 21 7 Hpn. natans (DSM 17985)* DSMZ 1130 Yes [43] 3 100 37 21 7 (B) Hfx. volcanii only ATCC 974 lower ------------------------------------------------------- higher Na + [M] 1 2 4 5 Mg 2+ [mM] 50 (75) (150) 250 (500) 1000 Temp.

Techniques: