Journal: Molecules

Article Title: The Anti-Multidrug-Resistant Acinetobacter baumannii Study on 1,3-diamino-7H-pyrrolo[3,2-f]quinazoline Compounds

doi: 10.3390/molecules27238609

Figure Lengend Snippet: MICs of OYYF-171, -172, -175, and TMP against ATCC strains of A. baumannii .

Article Snippet: , ATCC BAA-1791 , 1 , 1024 , 0.25 , 128 , 0.375 , , Synergism.

Techniques:

Journal: Molecules

Article Title: The Anti-Multidrug-Resistant Acinetobacter baumannii Study on 1,3-diamino-7H-pyrrolo[3,2-f]quinazoline Compounds

doi: 10.3390/molecules27238609

Figure Lengend Snippet: Checkerboard results of the combination of OYYF-171 with SMZ, against A. baumannii .

Article Snippet: , ATCC BAA-1791 , 1 , 1024 , 0.25 , 128 , 0.375 , , Synergism.

Techniques:

Journal: Biology Open

Article Title: In vivo genetic manipulation of cortical progenitors in gyrencephalic carnivores using in utero electroporation

doi: 10.1242/bio.20123160

Figure Lengend Snippet: In utero electroporation was performed at E37, and sections were prepared at E40. The sections were immunostained with anti-Sox2, anti-Pax6 and anti-Tbr2 antibodies. The cerebral cortex is shown in A . The areas within the white boxes are magnified and are shown in B . CP, cortical plate; OSVZ, outer subventricular zone; ISVZ, inner subventricular zone; VZ, ventricular zone. Scale bars: 200 µm (A) and 100 µm (B).

Article Snippet: Sections were made using a cryostat, permeabilized with 0.1–0.5% Triton X-100/PBS, and incubated overnight with primary antibodies, which included anti-Sox2 antibody (R&D Systems), anti-Pax6 antibody (Covance), anti-Tbr2 antibody (Abcam) and anti-neurofilament-M antibody (Chemicon).

Techniques: In Utero, Electroporation

Journal: Biology Open

Article Title: In vivo genetic manipulation of cortical progenitors in gyrencephalic carnivores using in utero electroporation

doi: 10.1242/bio.20123160

Figure Lengend Snippet: In utero electroporation was performed at E35, and coronal sections were prepared at P0 and were stained with Hoechst 33342. Many GFP-positive cells were distributed throughout the cortex ( A ). The areas within the white boxes in A are magnified and are shown in B . The areas within the white boxes in B are shown in C . Note that the morphology of GFP-positive cells was clearly visible even without immunostaining. ( D , E ) The sections were immunostained with anti-Sox2 antibody (D) and anti-Pax6 antibody (E), and high magnification images of the OSVZ are shown. The GFP-positive cells (arrowheads) expressed Sox2 (D) and Pax6 (E), and had basal fibers but not apical fibers, suggesting that these cell are oRG cells. GFP-positive fibers running tangentially in the inner OSVZ were also visible (arrow). *Lateral ventricle. CP, cortical plate; OSVZ, outer subventricular zone; ISVZ, inner subventricular zone; VZ, ventricular zone. Scale bars: 1 mm (A), 500 µm (B), 100 µm (C) and 20 µm (D,E).

Article Snippet: Sections were made using a cryostat, permeabilized with 0.1–0.5% Triton X-100/PBS, and incubated overnight with primary antibodies, which included anti-Sox2 antibody (R&D Systems), anti-Pax6 antibody (Covance), anti-Tbr2 antibody (Abcam) and anti-neurofilament-M antibody (Chemicon).

Techniques: In Utero, Electroporation, Staining, Immunostaining

Journal: bioRxiv

Article Title: BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells (MSCs)

doi: 10.1101/104927

Figure Lengend Snippet: (A) Mouse genomic snapshots of two representative genes (Klf4 and Foxf2) identified in RNA-seq analysis of Gli1-CreER;Bmpr1 α fl/fl apical molars, showing downregulation (blue) relative to their matched controls (red). Genomic location of each gene is shown below. Numbers in parentheses on the right indicate sequencing depth. (B) Pathway analysis from RNA-seq data. Each enriched pathway is ranked based on p-value that was computed from the biochemical distribution and independence for probability. The database is indicated in the parentheses.

Article Snippet: GFP control adenovirus (Ad-GFP, Cat. 1060) and Klf4 overexpression adenovirus (Ad-m-KLF4, 1791) were purchased from Vector Biolabs.

Techniques: RNA Sequencing, Sequencing

Journal: bioRxiv

Article Title: BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells (MSCs)

doi: 10.1101/104927

Figure Lengend Snippet: Immunostaining (red) of pSmad1/5/8 (A,B), Pax9 (C,D), Satb2 (I,J), and Klf4 (K,L) and in situ hybridization of Foxf2 (E,F) and Lhx8 (G,H) of P7.5 littermate Bmpr1 α fl/fl control and Gli1-CreER;Bmpr1 α fl/fl mice induced P3.5. A’-L’ are magnified images from A-L, respectively. Arrows indicate positive staining in control samples. Arrowheads indicate altered staining in targeted region of mutant samples. Scale bars: A-L, 100μm; A’-L’, 50μm.

Article Snippet: GFP control adenovirus (Ad-GFP, Cat. 1060) and Klf4 overexpression adenovirus (Ad-m-KLF4, 1791) were purchased from Vector Biolabs.

Techniques: Immunostaining, In Situ Hybridization, Control, Staining, Mutagenesis

Journal: bioRxiv

Article Title: BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells (MSCs)

doi: 10.1101/104927

Figure Lengend Snippet: (A-B) Ki67 and Klf4 immunostaining (red) of molars from P7.5 Gli1-CreER;Bmpr1 α fl/fl mice induced P3.5. Boxes in A and B are shown magnified in A’ and B’, respectively. (C) Western blot of Klf4 in cultured dental pulp cells from P7.5 Bmpr1 α fl/fl control mice treated with Bmp2 or Bmp4 or mock-treated (control). (D) Co-immunoprecipitation experiment using Flag-tagged Smad1 and HA-tagged Klf4 expressed in 293T cells. Smad1 was immunoprecipitated (IP) and immunoblotted (IB) for associated Klf4. (E-E’) Klf4 immunofluorescence after treatment of dissociated apical pulp culture for 48 hours with Ad-m-Klf4 (E) or ad-GFP (E’). (F) qPCR for Dspp in explant cultures treated with Ad-m-Klf4 (blue bar) compared with Ad-m-GFP (gray bar). N=3. *, p<0.05. Scale bars, 50μm.

Article Snippet: GFP control adenovirus (Ad-GFP, Cat. 1060) and Klf4 overexpression adenovirus (Ad-m-KLF4, 1791) were purchased from Vector Biolabs.

Techniques: Immunostaining, Western Blot, Cell Culture, Control, Immunoprecipitation, Immunofluorescence