17784 Search Results


tetrahydro furan 2 carbohydrazide commercially available from enamine kiev ukraine  (Enamine Ltd)
90
Enamine Ltd tetrahydro furan 2 carbohydrazide commercially available from enamine kiev ukraine
Tetrahydro Furan 2 Carbohydrazide Commercially Available From Enamine Kiev Ukraine, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tetrahydro furan 2 carbohydrazide commercially available from enamine kiev ukraine/product/Enamine Ltd
Average 90 stars, based on 1 article reviews
tetrahydro furan 2 carbohydrazide commercially available from enamine kiev ukraine - by Bioz Stars, 2026-02
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casp 9  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology casp 9
Casp 9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/casp 9/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
casp 9 - by Bioz Stars, 2026-02
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orc6  (Proteintech)
93
Proteintech orc6
ETV4 is located at the origins of LAMB2 and MCM4 DNA replication and plays a role in ORC loading at the origins of NSCLC cells. A) Gene body enrichment heat map of anti‐Flag ChIP‐seq signals in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids. B) Pie chart demonstrating the percentage of ETV4 peak distribution. C) Functional analysis showing the top KEGG pathways significantly associated with genes of ETV4 binding by ChIP‐seq assay. D,E) IGV tracks showing the co‐occupancy of ETV4 and c‐MYC with the chromatin accessibility at the LAMB2 origin and MCM4 origin (‐1Kb) regions from our ETV4 ChIP‐seq, MYC ChIP‐seq (ENCSR537FDJ), and ATAC‐seq (ENCSR220ASC) from A549 cells. F,G) ChIP‐qPCR assay showing the binding of ETV4 at LAMB2 origin and MCM4 origin regions in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids (mean ± SD, n = 3; two‐tailed unpaired t ‐test). * p < 0.05, ** p < 0.01. H,I) IP of ETV4 and Immunoblots against ORC1‐6 subunits from whole‐cell proteins or chromatin‐bound (Chrom.) proteins of A549 cells. J) Predicting model of the structural arrangement of ETV4 and ORC by superimposing the structure of ETV4 (PDB code 4co8) with the ORC complex (PDB code 7mca). dsDNA, double‐strand DNA. K,L) Schematic of the protocol for cells synchronized and released using a double thymidine block. The cell cycle distribution was detected by Flow cytometry. M) IP of ETV4 and Immunoblots against ORC1 and <t>ORC6</t> from thymidine block and released synchronized A549 cells. N,O) Quantification of ETV4‐ORC1 or ORC6 binding in M. P) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549 cells transfected with ORC6 siRNA or NC siRNA. (mean ± SD, n = 3; two‐tailed unpaired t ‐test) * p < 0.05, ** p < 0.01. Q) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549‐shETV4 cells transfected with ETV4 plasmids or combined with ORC6 siRNA, respectively. (mean ± SD, n = 3; one‐way ANOVA followed by Tukey's multiple comparisons test). * p < 0.05, ** p < 0.01.
Orc6, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/orc6/product/Proteintech
Average 93 stars, based on 1 article reviews
orc6 - by Bioz Stars, 2026-02
93/100 stars
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amobarbital cat# 17784  (Cayman Chemical)
90
Cayman Chemical amobarbital cat# 17784
ETV4 is located at the origins of LAMB2 and MCM4 DNA replication and plays a role in ORC loading at the origins of NSCLC cells. A) Gene body enrichment heat map of anti‐Flag ChIP‐seq signals in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids. B) Pie chart demonstrating the percentage of ETV4 peak distribution. C) Functional analysis showing the top KEGG pathways significantly associated with genes of ETV4 binding by ChIP‐seq assay. D,E) IGV tracks showing the co‐occupancy of ETV4 and c‐MYC with the chromatin accessibility at the LAMB2 origin and MCM4 origin (‐1Kb) regions from our ETV4 ChIP‐seq, MYC ChIP‐seq (ENCSR537FDJ), and ATAC‐seq (ENCSR220ASC) from A549 cells. F,G) ChIP‐qPCR assay showing the binding of ETV4 at LAMB2 origin and MCM4 origin regions in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids (mean ± SD, n = 3; two‐tailed unpaired t ‐test). * p < 0.05, ** p < 0.01. H,I) IP of ETV4 and Immunoblots against ORC1‐6 subunits from whole‐cell proteins or chromatin‐bound (Chrom.) proteins of A549 cells. J) Predicting model of the structural arrangement of ETV4 and ORC by superimposing the structure of ETV4 (PDB code 4co8) with the ORC complex (PDB code 7mca). dsDNA, double‐strand DNA. K,L) Schematic of the protocol for cells synchronized and released using a double thymidine block. The cell cycle distribution was detected by Flow cytometry. M) IP of ETV4 and Immunoblots against ORC1 and <t>ORC6</t> from thymidine block and released synchronized A549 cells. N,O) Quantification of ETV4‐ORC1 or ORC6 binding in M. P) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549 cells transfected with ORC6 siRNA or NC siRNA. (mean ± SD, n = 3; two‐tailed unpaired t ‐test) * p < 0.05, ** p < 0.01. Q) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549‐shETV4 cells transfected with ETV4 plasmids or combined with ORC6 siRNA, respectively. (mean ± SD, n = 3; one‐way ANOVA followed by Tukey's multiple comparisons test). * p < 0.05, ** p < 0.01.
Amobarbital Cat# 17784, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amobarbital cat# 17784/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
amobarbital cat# 17784 - by Bioz Stars, 2026-02
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nr 17784  (BEI Resources)
90
BEI Resources nr 17784
ETV4 is located at the origins of LAMB2 and MCM4 DNA replication and plays a role in ORC loading at the origins of NSCLC cells. A) Gene body enrichment heat map of anti‐Flag ChIP‐seq signals in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids. B) Pie chart demonstrating the percentage of ETV4 peak distribution. C) Functional analysis showing the top KEGG pathways significantly associated with genes of ETV4 binding by ChIP‐seq assay. D,E) IGV tracks showing the co‐occupancy of ETV4 and c‐MYC with the chromatin accessibility at the LAMB2 origin and MCM4 origin (‐1Kb) regions from our ETV4 ChIP‐seq, MYC ChIP‐seq (ENCSR537FDJ), and ATAC‐seq (ENCSR220ASC) from A549 cells. F,G) ChIP‐qPCR assay showing the binding of ETV4 at LAMB2 origin and MCM4 origin regions in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids (mean ± SD, n = 3; two‐tailed unpaired t ‐test). * p < 0.05, ** p < 0.01. H,I) IP of ETV4 and Immunoblots against ORC1‐6 subunits from whole‐cell proteins or chromatin‐bound (Chrom.) proteins of A549 cells. J) Predicting model of the structural arrangement of ETV4 and ORC by superimposing the structure of ETV4 (PDB code 4co8) with the ORC complex (PDB code 7mca). dsDNA, double‐strand DNA. K,L) Schematic of the protocol for cells synchronized and released using a double thymidine block. The cell cycle distribution was detected by Flow cytometry. M) IP of ETV4 and Immunoblots against ORC1 and <t>ORC6</t> from thymidine block and released synchronized A549 cells. N,O) Quantification of ETV4‐ORC1 or ORC6 binding in M. P) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549 cells transfected with ORC6 siRNA or NC siRNA. (mean ± SD, n = 3; two‐tailed unpaired t ‐test) * p < 0.05, ** p < 0.01. Q) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549‐shETV4 cells transfected with ETV4 plasmids or combined with ORC6 siRNA, respectively. (mean ± SD, n = 3; one‐way ANOVA followed by Tukey's multiple comparisons test). * p < 0.05, ** p < 0.01.
Nr 17784, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nr 17784/product/BEI Resources
Average 90 stars, based on 1 article reviews
nr 17784 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


ETV4 is located at the origins of LAMB2 and MCM4 DNA replication and plays a role in ORC loading at the origins of NSCLC cells. A) Gene body enrichment heat map of anti‐Flag ChIP‐seq signals in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids. B) Pie chart demonstrating the percentage of ETV4 peak distribution. C) Functional analysis showing the top KEGG pathways significantly associated with genes of ETV4 binding by ChIP‐seq assay. D,E) IGV tracks showing the co‐occupancy of ETV4 and c‐MYC with the chromatin accessibility at the LAMB2 origin and MCM4 origin (‐1Kb) regions from our ETV4 ChIP‐seq, MYC ChIP‐seq (ENCSR537FDJ), and ATAC‐seq (ENCSR220ASC) from A549 cells. F,G) ChIP‐qPCR assay showing the binding of ETV4 at LAMB2 origin and MCM4 origin regions in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids (mean ± SD, n = 3; two‐tailed unpaired t ‐test). * p < 0.05, ** p < 0.01. H,I) IP of ETV4 and Immunoblots against ORC1‐6 subunits from whole‐cell proteins or chromatin‐bound (Chrom.) proteins of A549 cells. J) Predicting model of the structural arrangement of ETV4 and ORC by superimposing the structure of ETV4 (PDB code 4co8) with the ORC complex (PDB code 7mca). dsDNA, double‐strand DNA. K,L) Schematic of the protocol for cells synchronized and released using a double thymidine block. The cell cycle distribution was detected by Flow cytometry. M) IP of ETV4 and Immunoblots against ORC1 and ORC6 from thymidine block and released synchronized A549 cells. N,O) Quantification of ETV4‐ORC1 or ORC6 binding in M. P) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549 cells transfected with ORC6 siRNA or NC siRNA. (mean ± SD, n = 3; two‐tailed unpaired t ‐test) * p < 0.05, ** p < 0.01. Q) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549‐shETV4 cells transfected with ETV4 plasmids or combined with ORC6 siRNA, respectively. (mean ± SD, n = 3; one‐way ANOVA followed by Tukey's multiple comparisons test). * p < 0.05, ** p < 0.01.

Journal: Advanced Science

Article Title: Topoisomerase I Inhibition in ETV4‐overexpressed Non‐Small Cell Lung Cancer Promotes Replication and Transcription Mediated R‐Loop Accumulation and DNA Damage

doi: 10.1002/advs.202409307

Figure Lengend Snippet: ETV4 is located at the origins of LAMB2 and MCM4 DNA replication and plays a role in ORC loading at the origins of NSCLC cells. A) Gene body enrichment heat map of anti‐Flag ChIP‐seq signals in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids. B) Pie chart demonstrating the percentage of ETV4 peak distribution. C) Functional analysis showing the top KEGG pathways significantly associated with genes of ETV4 binding by ChIP‐seq assay. D,E) IGV tracks showing the co‐occupancy of ETV4 and c‐MYC with the chromatin accessibility at the LAMB2 origin and MCM4 origin (‐1Kb) regions from our ETV4 ChIP‐seq, MYC ChIP‐seq (ENCSR537FDJ), and ATAC‐seq (ENCSR220ASC) from A549 cells. F,G) ChIP‐qPCR assay showing the binding of ETV4 at LAMB2 origin and MCM4 origin regions in A549‐shETV4 cells transfected with Flag‐ETV4 plasmids (mean ± SD, n = 3; two‐tailed unpaired t ‐test). * p < 0.05, ** p < 0.01. H,I) IP of ETV4 and Immunoblots against ORC1‐6 subunits from whole‐cell proteins or chromatin‐bound (Chrom.) proteins of A549 cells. J) Predicting model of the structural arrangement of ETV4 and ORC by superimposing the structure of ETV4 (PDB code 4co8) with the ORC complex (PDB code 7mca). dsDNA, double‐strand DNA. K,L) Schematic of the protocol for cells synchronized and released using a double thymidine block. The cell cycle distribution was detected by Flow cytometry. M) IP of ETV4 and Immunoblots against ORC1 and ORC6 from thymidine block and released synchronized A549 cells. N,O) Quantification of ETV4‐ORC1 or ORC6 binding in M. P) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549 cells transfected with ORC6 siRNA or NC siRNA. (mean ± SD, n = 3; two‐tailed unpaired t ‐test) * p < 0.05, ** p < 0.01. Q) ChIP‐qPCR assay showing the binding of ORC1 at LAMB2 and MCM4 origins in A549‐shETV4 cells transfected with ETV4 plasmids or combined with ORC6 siRNA, respectively. (mean ± SD, n = 3; one‐way ANOVA followed by Tukey's multiple comparisons test). * p < 0.05, ** p < 0.01.

Article Snippet: Blots were blocked with 5% nonfat milk in Tris‐Buffered Saline and Tween 20 (TBST), and incubated with antibodies specific for ETV4 (10684‐1‐AP, Proteintech), MCM2 (3619, CST), MCM3 (15597‐1‐AP, Proteintech), MCM4 (13043‐1‐AP, Proteintech), MCM5 (11703‐1‐AP, Proteintech), MCM6 (13347‐2‐AP, Proteintech), MCM7 (11225‐1‐AP, Proteintech), MCM10 (12251‐1‐AP, Proteintech), ORC1 (NBP100‐121, Novus), ORC2 (12739‐1‐AP, Proteintech), ORC3 (sc‐374231, Santa Cruz), ORC4 (13026‐1‐AP, Proteintech), ORC5 (11542‐1‐AP, Proteintech), ORC6 (17784‐1‐AP, Proteintech), Histone‐H3 (17168‐1‐AP, Proteintech), PCNA (13110, CST), phospho‐MCM2 S40 (ab133243, Abcam), CDC45 (15678‐1‐AP, Proteintech), SUPT16H (28598‐1‐AP, Proteintech), SSRP1 (15696‐1‐AP, Proteintech), γ‐H2AX (ET‐1602, HUABio), Flag (14793, CST), His (66005‐1‐Ig, Proteintech), HA (sc‐7392, Santa Cruz), GST (66001‐2‐lg, Proteintech), GAPDH (60004‐1‐Ig, Proteintech) and β‐actin (66009‐1‐Ig, Proteintech) was used as a loading control for Western blot.

Techniques: ChIP-sequencing, Transfection, Functional Assay, Binding Assay, ChIP-qPCR, Two Tailed Test, Western Blot, Blocking Assay, Flow Cytometry