Journal: Journal of Translational Medicine

Article Title: Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients

doi: 10.1186/s12967-025-06100-6

Figure Lengend Snippet: The pro-inflammatory HDL from ARDS patients showed marked changes in protein profile by proteomic analysis. The plasma samples from 8 ARDS patients and 8 healthy controls were collected. The plasma mixture from 2 subjects with similar ages and clinical situations was used for the isolation procedure to improve the quality. A The DEPs between N-HDL and A-HDL by DIA-MS proteome analysis. There was significant specificity between N-HDL and A-HDL by PCA. B , C The volcano plot and heatmap showed that a total of 384 proteins were identified with 53 upregulated and 49 downregulated proteins between A-HDL and N-HDL. The blue and red-colored proteins denoted the 18 HDL proteins with the most significant fold-changes. D The Gene Ontology analysis on these DEPs and the most affected processes were presented. ARDS, acute respiratory distress syndrome; HDL, high-density lipoprotein; N-HDL, HDL from normal subjects; A-HDL, HDL from ARDS patients; DEPs, differentially expressed proteins; DIA-MS, data-independent acquisition mass spectrometry; PCA, principal component analysis; SFTPB, pulmonary surfactant-associated protein B; SAA, serum amyloid A; Apo, apolipoprotein; HPR, haptoglobin-related protein; PON, paraoxonase; SERPIN, serpin family A member; IGFBP3, insulin-like growth factor-binding protein 3; LBP, lipopolysaccharide-binding protein; LPA, apolipoprotein(a); SA100A8, S100 calcium-binding protein A8; LGALS3BP, lectin, galactoside-binding, soluble 3 binding protein; ITIH4, inter-alpha-trypsin inhibitor 4

Article Snippet: HDL (1 mg/ml, 250 μg, Sigma, L1567) was incubated with 16.5 μg recombinant human SFTPB (0.066 mg/ml, Sino Biological, 17,682-H08C1) in PBS containing 10 mM of reduced glutathione at 37 ℃ for 2 h. Then, the mixture was reisolated with ultrafiltration tubes to remove free SFTPB.

Techniques: Isolation, Mass Spectrometry, Binding Assay

Journal: Journal of Translational Medicine

Article Title: Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients

doi: 10.1186/s12967-025-06100-6

Figure Lengend Snippet: The altered HDL proteins identified in the discovery cohort were confirmed in the validation cohort. A Flowchart of ARDS patient enrollment along with healthy controls. B The significantly changed HDL constituent proteins in A-HDL versus N-HDL in the validation cohort. ARDS, acute respiratory distress syndrome; HDL, high-density lipoprotein; N-HDL, HDL from normal subjects; A-HDL, HDL from ARDS patients; pro-SFTPB, pro-pulmonary surfactant-associated protein B; Apo, apolipoprotein; SAA, serum amyloid A; PON, paraoxonase; LBP, lipopolysaccharide binding protein; SERPINA5, serpin family A member 5; LGALS3BP, lectin galactoside-binding soluble 3 binding protein; **p < 0.01; ***p < 0.001; ****p < 0.0001

Article Snippet: HDL (1 mg/ml, 250 μg, Sigma, L1567) was incubated with 16.5 μg recombinant human SFTPB (0.066 mg/ml, Sino Biological, 17,682-H08C1) in PBS containing 10 mM of reduced glutathione at 37 ℃ for 2 h. Then, the mixture was reisolated with ultrafiltration tubes to remove free SFTPB.

Techniques: Binding Assay

Journal: Journal of Translational Medicine

Article Title: Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients

doi: 10.1186/s12967-025-06100-6

Figure Lengend Snippet: Pro-SFTPB in HDL is associated with the prognosis of ARDS patients. A Only pro-SFTPB in HDL showed a significant difference between survivors (n = 12) and non-survivors (n = 10). B ROC curves for pro-SFTPB in HDL and SOFA score in prediction of the 28-day mortality in ARDS patients. ARDS, acute respiratory distress syndrome; HDL, high density lipoprotein; Pro-SFTPB, pro-pulmonary surfactant-associated protein B; Apo, apolipoprotein; SAA, serum amyloid A; PON, paraoxonase; LBP, lipopolysaccharide binding protein; SERPINA5, serpin family A member 5; LGALS3BP, lectin galactoside-binding soluble 3 binding protein; ROC, receiver operating characteristic; AUC, area under the curve; CI, confidence interval; SOFA, sequential organ failure assessment; *p < 0.05

Article Snippet: HDL (1 mg/ml, 250 μg, Sigma, L1567) was incubated with 16.5 μg recombinant human SFTPB (0.066 mg/ml, Sino Biological, 17,682-H08C1) in PBS containing 10 mM of reduced glutathione at 37 ℃ for 2 h. Then, the mixture was reisolated with ultrafiltration tubes to remove free SFTPB.

Techniques: Binding Assay

Journal: Journal of Translational Medicine

Article Title: Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients

doi: 10.1186/s12967-025-06100-6

Figure Lengend Snippet: Correlation analysis between the HDL-pro-SFTPB level and cytokines

Article Snippet: HDL (1 mg/ml, 250 μg, Sigma, L1567) was incubated with 16.5 μg recombinant human SFTPB (0.066 mg/ml, Sino Biological, 17,682-H08C1) in PBS containing 10 mM of reduced glutathione at 37 ℃ for 2 h. Then, the mixture was reisolated with ultrafiltration tubes to remove free SFTPB.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients

doi: 10.1186/s12967-025-06100-6

Figure Lengend Snippet: Correlation analysis between the pro-SFTPB levels in HDL and other HDL proteins

Article Snippet: HDL (1 mg/ml, 250 μg, Sigma, L1567) was incubated with 16.5 μg recombinant human SFTPB (0.066 mg/ml, Sino Biological, 17,682-H08C1) in PBS containing 10 mM of reduced glutathione at 37 ℃ for 2 h. Then, the mixture was reisolated with ultrafiltration tubes to remove free SFTPB.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients

doi: 10.1186/s12967-025-06100-6

Figure Lengend Snippet: Scatter diagram for the relationship between pro-SFTPB and SAA2 and PON3 in HDL. pro-SFTPB, pro-pulmonary surfactant-associated protein B; SAA2, serum amyloid A-2 protein; PON3, serum paraoxonase 3; HDL, high density lipoprotein

Article Snippet: HDL (1 mg/ml, 250 μg, Sigma, L1567) was incubated with 16.5 μg recombinant human SFTPB (0.066 mg/ml, Sino Biological, 17,682-H08C1) in PBS containing 10 mM of reduced glutathione at 37 ℃ for 2 h. Then, the mixture was reisolated with ultrafiltration tubes to remove free SFTPB.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients

doi: 10.1186/s12967-025-06100-6

Figure Lengend Snippet: Pro-SFTPB enriched HDL induced M1 macrophage polarization. A Schematic diagram of HDL-pro-SFTPB (PS-HDL) synthesis. B Immunoblotting of HDL-pro-SFTPB showed that HDL successfully carried pro-SFTPB. C The PS-HDL treatment (100 μg/ml, 12 h) induced M1 polarization of THP-1 derived macrophage by qPCR assay of M1/M2-associated markers. (n = 5 per group). D The phagocytic capability of THP-1 derived macrophages by quantifying the uptake of labeled E. coli particles after HDL and PS-HDL treatments (100 μg/ml, 12 h), the percentage of particle-positive cells was presented as bar figure (n = 4 per group). E intracellular ROS release in THP-1 derived macrophages 30 min after HDL exposure was quantified via the changes in DCF fluorescence (n = 5 per group). HDL, high density lipoprotein; Pro-SFTPB, pro-pulmonary surfactant-associated protein B; C-H: control HDL; PS-HDL, Pro-SFTPB enriched HDL; TNF, tumor necrosis factor; IL-, interleukin-; MRC-1, mannose receptor C-type 1; ROS, reactive oxygen species; **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: HDL (1 mg/ml, 250 μg, Sigma, L1567) was incubated with 16.5 μg recombinant human SFTPB (0.066 mg/ml, Sino Biological, 17,682-H08C1) in PBS containing 10 mM of reduced glutathione at 37 ℃ for 2 h. Then, the mixture was reisolated with ultrafiltration tubes to remove free SFTPB.

Techniques: Western Blot, Derivative Assay, Labeling, Fluorescence, Control

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Primers used for PCR amplification of Taenia multiceps annexins .

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Amplification

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Primers used for quantitative real-time PCR (qRT-PCR) amplification of T. multiceps annexins.

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Real-time Polymerase Chain Reaction, Amplification

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Sequence alignment analyses of: AnxB2 ( A ); AnxB3 ( B ); and AnxB12 ( C ) in T. multiceps and other species. ( A ) The following sequences were retrieved from the GenBank protein sequence database (accession numbers are indicated in parentheses): Em, Echinococcus multilocularis (CDI98110.1); Eg, Echinococcus granulosus (CDS21833.1); Ts, Taenia solium (AAY17503.1); Ht, Hydatigena taeniaeformis (A0A0R3X1T1); Mc, Mesocestoides corti (A0A0R3UMC3); Hm, Hymenolepis microstoma (CDS30725.1); Se, Spirometra erinaceieuropaei (ADM26238.1); Sj, Schistosoma japonicum (AAW25344.1); Sm, Schistosoma mansoni (AAC79802.3); Hs, Homo sapiens (AAH00871.1); Cs, Clonorchis sinensis (GAA48684.1); ( B ) The following sequences were retrieved from the GenBank protein sequence database (accession numbers are indicated in parentheses): Ts, Taenia solium (AAY27744.1); Em, Echinococcus multilocularis (CDI98572.1); Eg, Echinococcus granulosus (EUB59082.1); Mc, Mesocestoides corti (A0A0R3UJ25); Hm, Hymenolepis microstoma (CDS27549.1); Cs, C. sinensis (GAA33818.2); Sh, Schistosoma haematobium (KGB40261.1); Ch, Capra hicus (XP_013824596.1); Hs, Homo sapiens (CAG46637.1); Mm, Mus musculus (NP_081487.1); Sm, S. mansoni (AAC79802.3); ( C ) The following sequences were retrieved from the GenBank protein sequence database (accession numbers are indicated in parentheses): Ta, Taenia asiatica (A0A0R3W340); Eg, E. granulosus (EUB63408.1); Em, Echinococcus multilocularis (CDI98522.1); Hd, Hymenolepis diminuta (A0A0R3SPK4); Hm, Hymenolepis microstoma (CDS26802.2); Sh, Schistosoma haematobium (XP_012800019.1); Sm, S. mansoni (XP_018651719.1); Sj, Schistosoma japonicum (CAX69693.1); Hs, Homo sapiens (AAH00871.1). The yellow arrow in the figure represents the K-G-X-G-T sequence; the black box is the KGD sequence.

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Sequencing

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Phylogenetic analysis of annexin proteins. Phylogenetic analysis of the full-length amino acid sequences of T. multiceps annexins (in red boxes) and their homologs. The tree was constructed by the maximum-likelihood (ML) method and plotted with PhyML 3.1 with the Jones–Taylor–Thornton model selected by ProtTest 3 ( http://darwin.uvigo.es/our-software/ ). Bootstrap values are indicated at the nodes (1000 replications). The scale indicates an estimate of substitutions per site, using the optimized model setting.

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Construct, Software

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Purification and Western blotting of recombinant rTmAnxB2. M, Protein markers; 1, pET32a-vector; 2, pET32a-TmAnxB2; 3, Purified rTmAnxB2; 4, rTmAnxB2 detected by T. multiceps -positive goat serum; 5, rTmAnxB2 detected by T. multiceps -negative goat serum; 6, Western blot of T. multiceps extracts detected by rabbit anti-rTmAnxB2-IgG; 7, Western blot of T. multiceps extracts detected by healthy rabbit serum (controls).

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Purification, Western Blot, Recombinant, Plasmid Preparation

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Purification and Western blotting of recombinant rTmAnxB3. M, Protein markers; 1, pET32a-vector; 2, pET32a-TmAnxB3; 3, Purified rTmAnxB3; 4, rTmAnxB3 detected by T. multiceps -positive goat serum; 5, rTmAnxB3 detected by T. multiceps -negative goat serum; 6: Western blot of T. multiceps extracts detected by rabbit anti-rTmAnxB3-IgG; 7, Western blot of T. multiceps extracts detected by healthy rabbit serum.

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Purification, Western Blot, Recombinant, Plasmid Preparation

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Immunofluorescence localization of TmAnxB2 in T. multiceps . Against the red background, the green fluorescent color shows the location of the native TmAnxB2 protein: ( A ) protoscolex with positive serum; ( B ) protoscolex with negative serum; ( C ) cyst wall with positive serum; ( D ) cyst wall with negative serum; ( E ) gravid proglottids with positive serum; and ( F ) gravid proglottids with negative serum; GL, germinal layer; HL, horny layer; Eg, eggs.

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Immunofluorescence

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Immunofluorescence localization of TmAnxB3 in T. multiceps . Against the red background, the green fluorescent color shows the location of the native TmAnxB3 protein: ( A ) protoscolex with positive serum; ( B ) protoscolex with negative serum; ( C ) cyst wall with positive serum; ( D ) cyst wall with negative serum; GL, germinal layer; HL, horny layer; ( E ) scolex with positive serum; ( F ) scolex with negative serum; ( G ) neck with positive serum; ( H ) neck with negative serum; ( I ) immature proglottids with positive serum; ( J ) immature proglottids with negative serum; ( K ) mature proglottids with positive serum; ( L ) mature proglottids with negative serum; ( M ) gravid proglottids with positive serum; and ( N ) gravid proglottids with negative serum. Abbreviations: S, suckers; GL, germinal layer; HL, horny layer; TZ, tegument zone; PZ, parenchymatous zone; ED, excretory duct; U, Uterus; Eg, eggs.

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Immunofluorescence

Journal: Genes

Article Title: Molecular Characterization of Annexin B2, B3 and B12 in Taenia multiceps

doi: 10.3390/genes9110559

Figure Lengend Snippet: Differential transcription levels of TmAnxB2, TmAnxB3 and TmAnxB12 in different proglottids and developmental stages of T. multiceps . Levels of expression were determined using the ΔΔ Ct method. Data are represented as the means ± SD ( n = 3). Asterisks indicate fold changes that were statistically significant at p < 0.05.

Article Snippet: Gene-specific primers for TmAnxB12 were designed using the T. multiceps transcriptome dataset (Unigene 17682) and an annexin from Echinococcus granulosus (GenDB No. EgrG_000237700).

Techniques: Expressing