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Image Search Results
Journal: bioRxiv
Article Title: Multiple Epigenetic Mechanisms Functionally Cooperate to Silence Expression of Somatostatin Receptor Type 2 in Pancreatic Neuroendocrine Tumors
doi: 10.1101/2025.09.23.677935
Figure Lengend Snippet: (A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting DNMT1, DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).
Article Snippet: Primary antibodies utilized were: SSTR2 (BosterBio, M01689),
Techniques: Western Blot, Expressing, Knockdown, shRNA, Methylation, Control, CpG Methylation Assay