1700 Search Results


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scp  (ATCC)
93
ATCC scp
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EpiGentek dnmt1
(A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting <t>DNMT1,</t> DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).
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Mini-Circuits minicircuits pos 150 vco reference
(A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting <t>DNMT1,</t> DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).
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Mini-Circuits low pass filters
(A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting <t>DNMT1,</t> DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).
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JASCO Inc jasco uv 975 uv detector
(A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting <t>DNMT1,</t> DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).
Jasco Uv 975 Uv Detector, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mini-Circuits mini circuits vlfg
(A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting <t>DNMT1,</t> DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).
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Image Search Results


(A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting DNMT1, DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).

Journal: bioRxiv

Article Title: Multiple Epigenetic Mechanisms Functionally Cooperate to Silence Expression of Somatostatin Receptor Type 2 in Pancreatic Neuroendocrine Tumors

doi: 10.1101/2025.09.23.677935

Figure Lengend Snippet: (A) Immunoblot analysis of BON-1 and QGP-1 cells treated with the DNMT inhibitor, Decitabine (400 nM for 72-hrs) shows increased expression of total SSTR2 protein. (B) Immunoblot analysis confirms stable knockdown of DNMTs in BON-1 cells using pre-validated shRNA targeting DNMT1, DNMT3A and DNMT3B. Specific percent stable knockdown of each DNMT is noted (similar knockdown results in QGP-1 cells). (C) Immunoblot analysis reveals that stable knockdown of DNMT3B in both BON-1 and QGP-1 cells selectively increases expression of total SSTR2 protein. (D) Schematic of the human SSTR2 gene promoter element and CpG sites (red lines) having significantly decreased methylation levels (P < 0.05, at the least) after stable knockdown of DNMT3B in BON-1 and QGP-1 cells, compared to cells expressing control non-targeting shRNA (n=3). (E) Total protein levels in BON-1, QGP-1 and NT-3 cells correlates with SSTR2 gene promoter CpG methylation levels (n=3).

Article Snippet: Primary antibodies utilized were: SSTR2 (BosterBio, M01689), DNMT1 (Epigentek, A-1700), DNMT3A (Santa Cruz Biotechnology, sc-365769), DNMT3B (Proteintech, 26971-1-AP), LSH/HELLS (Proteintech, 11955-1-AP) and HiBiT (Promega, N7200).

Techniques: Western Blot, Expressing, Knockdown, shRNA, Methylation, Control, CpG Methylation Assay