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Image Search Results
Journal: Agronomy
Article Title: Cowpea: Prospecting for Heat-Tolerant Genotypes
doi: 10.3390/agronomy14091969
Figure Lengend Snippet: Figure 1. Genetic variability of seed morphology and size of studied cowpea genotypes. GN1: BRS Inhuma; GN2: Bico-de-Ouro-17-10; GN3: Pingo-de-Ouro-17-18; GN4: Bico-de-Oouro-17-19; GN5: Bico-de-Ouro-17-20; GN6: Bico-de-Ouro-17-44; GN7: Bico-de-Ouro-17-45; GN8: Bico-de-Ouro-17-46; GN9: Bico-de-Ouro-17-47; GN10: Pingo-de-Ouro-17-48; GN11: Bico-de-Ouro-17-72; G12: BRS Guariba; GN13: Bico-de-Ouro-17-82; GN14: Bico-de-Ouro-17-86; GN15: MNC01-631F-20-5; GN16: MNC00-595F-27; GN17: BRS Imponente; GN18: MNC06-895E-1; GN19: MNC09-981B-2; GN20: BRS Paraguaçu.
Article Snippet: GN1: BRS Inhuma; GN2: Bico-de-Ouro-17-10; GN3: Pingo-de-Ouro-17-18;
Techniques:
Journal: Nature Communications
Article Title: Microbiome-derived bile acid signatures in early life and their association with islet autoimmunity
doi: 10.1038/s41467-025-66619-6
Figure Lengend Snippet: a Schematic of the Th17 and iTreg differentiation protocol for primary human naïve CD4 + CD25 − T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 − T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. Illustrations in a panel were created with BioRender.com (licensed). b – e IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f – i . Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for six biological replicates. Statistical significance was determined using a paired, two-tailed Student’s t-test. Illustrations were Created in BioRender. Hirvonen, K. (2025) https://BioRender.com/5s3bnh7 . Asn–UDCA asparagine–ursodeoxycholic acid, Ser–CDCA serine–chenodeoxycholic acid, Tyr–CDCA tyrosine–chenodeoxycholic acid, UDCA ursodeoxycholic acid.
Article Snippet: To study the effect of MCBAs on human Th17 and iTreg cell differentiation, 100 μM of either Asn-UDCA, Ser-CDCA, Tyr-CDCA, or UDCA (in DMSO), and DMSO as a control, were added to the Th17 and iTreg cell culture media at day 0, and cultured for 72 h. After differentiation, secreted IL-17a levels were determined from Th17 cell-culture supernatants at 72 h using the
Techniques: Isolation, Control, Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture, Flow Cytometry, Fluorescence, Two Tailed Test