Journal: The Journal of biological chemistry

Article Title: Characterization of the substrate binding site in rat liver 3alpha-hydroxysteroid/dihydrodiol dehydrogenase. The roles of tryptophans in ligand binding and protein fluorescence.

doi: 10.1074/jbc.271.47.30190

Figure Lengend Snippet: FIG. 7. Model of androstanedione binding in the substrate binding pocket of rat liver 3a-HSD. This view displays the residues of the steroid hormone binding site in relation to the active site residues (Asp50, Tyr55, and Lys84) and bound NADP1 cofactor (17). The location of Trp86

Article Snippet: Androsterone, androstanedione, and testosterone were obtained from Steraloids.

Techniques: Binding Assay

Journal: Infectious agents and cancer

Article Title: Silencing of UBE2D1 inhibited cell migration in gastric cancer, decreasing ubiquitination of SMAD4.

doi: 10.1186/s13027-021-00402-2

Figure Lengend Snippet: Fig. 1 UBE2D1 was elevated in GC tissues. A The mRNA level of UBE2D1 was analyzed from TCGA-STAD data. B The mRNA level of UBE2D1 was detected in 25 pairs of tumors and paracancerous samples. ***, P < 0.001. C The protein level of UBE2D1 in tissue array (32 pairs) was determined by IHC staining. D Impact of UBE2D1 expression on OS in GC patients in TCGA

Article Snippet: Primary antibodies were listed as following: UBE2D1 (1:800, Proteintech, Rosemont, IL, USA), SMAD4 (1:1000, Abcam, St. Louis, MO, USA), MMP2 (1:1000, Abcam), MMP9 (1:800, Abcam), and β-actin (1:2000, Abcam).

Techniques: Immunohistochemistry, Expressing

Journal: Infectious agents and cancer

Article Title: Silencing of UBE2D1 inhibited cell migration in gastric cancer, decreasing ubiquitination of SMAD4.

doi: 10.1186/s13027-021-00402-2

Figure Lengend Snippet: Fig. 2 Expression level of UBE2D1 was measured by real-time PCR western blot assay. A UBE2D1 expression level in different cell lines, including GSE-1, AGS, BGC-823, MGC-803, MKN45, and SGC-7901. B Knock-down efficiency was evaluated after transduction with shUBE2D1 lentiviruses in AGS and MKN45 cells. ***, P < 0.001 versus shNC. C Overexpression efficiency was evaluated after transduction with UBE2D1 overexpression lentivirus in MGC-803 cells. ***, P < 0.001 versus vector

Article Snippet: Primary antibodies were listed as following: UBE2D1 (1:800, Proteintech, Rosemont, IL, USA), SMAD4 (1:1000, Abcam, St. Louis, MO, USA), MMP2 (1:1000, Abcam), MMP9 (1:800, Abcam), and β-actin (1:2000, Abcam).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Knockdown, Transduction, Over Expression, Plasmid Preparation

Journal: Infectious agents and cancer

Article Title: Silencing of UBE2D1 inhibited cell migration in gastric cancer, decreasing ubiquitination of SMAD4.

doi: 10.1186/s13027-021-00402-2

Figure Lengend Snippet: Fig. 4 Overexpression of UBE2D1 promoted cell migration in vitro. A–C Transwell (A and B) and wound healing (C) assays were performed to analyze cell migration after transduction with UBE2D1 overexpression lentivirus in MGC-803 cells. ***, P < 0.001 versus vector. D A western blot assay was performed to measure the protein levels of UBE2D1, MMP2, and MMP9

Article Snippet: Primary antibodies were listed as following: UBE2D1 (1:800, Proteintech, Rosemont, IL, USA), SMAD4 (1:1000, Abcam, St. Louis, MO, USA), MMP2 (1:1000, Abcam), MMP9 (1:800, Abcam), and β-actin (1:2000, Abcam).

Techniques: Over Expression, Migration, In Vitro, Transduction, Plasmid Preparation, Western Blot