Journal:

Article Title: Pneumolysin Detection Identifies Atypical Isolates of Streptococcus pneumoniae

doi:

Figure Lengend Snippet: Results of confirmatory tests on five isolates of S. pneumoniae with atypical colonial appearances

Article Snippet: We therefore examined further these five isolates (i) by a real-time PCR method for the pneumolysin gene which included a hybridization probe for amplicon recognition ( 4 ), (ii) by demonstrating the expression of pneumolysin in the lysed organisms using a monoclonal antibody to a recombinant pneumolysin in a Western blot technique ( 8 ), and (iii) by a commercial 16S RNA gene probe (AccuProbe; Gene-Probe Inc., San Diego, Calif.).

Techniques: Western Blot, Solubility

Journal: 3 Biotech

Article Title: Isolation and characterization of a newly naphthalene-degrading Halomonas pacifica, strain Cnaph3: biodegradation and biosurfactant production studies

doi: 10.1007/s13205-020-2085-x

Figure Lengend Snippet: The differential phenotypic characteristics of the strain Cnaph3 and other related strains of the genus Halomonas

Article Snippet: Its 16S rRNA gene sequence, comprising 1403 nucleotides, was deposited in the nucleotide database GenBank under accession number {"type":"entrez-nucleotide","attrs":{"text":"KX946967","term_id":"1073581319"}} KX946967 . table ft1 table-wrap mode="anchored" t5 Table 3 caption a7 Characteristics Strain Cnaph3 Halomonas pacifica DSM 7474 T (Baumann et al. 1983 ) Halomonas salifodinae JCM 14803 T (Wang et al. 2008 ) Halomonas elongata ATCC33173 T (Vreeland et al. 1980 ) Halomonas eurihalina ATCC 49336 T (Quesada et al. 1990 ) 16S RNA similarity (%) 100 98.7 98.4 94.5 93.5 Colony pigmentation Cream Cream Brown-yellow White Cream Morphology Rods Rods Long rods Rods Rods Cell size (μm) 0.5–0.7*1.5–2.0 0.6–0.8*1.6–1.9 0.8–1.2*4.0–6.0 nd 0.8–1.0*2.0–2.5 Motility + + + + + Gram stain − − − − − Oxidase + + + + − Catalase + + + + nd Growth NaCl range (%, w/v) 0–20 0–20 0.5–20 0–20 0.5–25 Optimum NaCl (%, w/v) 3 0.5–3 3 3–8 7.5 Growth temperature range (°C) 30–45 4–45 4–48 4–45 4–45 Optimum temperature (°C) 37 27 30 30 32 Growth pH range 6.5–10 5–10 6–9 5–10 5–10 Optimum pH 7 nd 7 7.5 7.2 Growth on l -Arabinose − − − + − d -Galactose nd + nd − − d -Glucose + + + + − Maltose − − + + − d -Mannitol + + + + − d -Mannose + + nd − − Origin Seawater taken from the Ataya’s fishing harbor, Kerkennah islands, Tunisia Seawater, Hawaii Saline soil sample collected from a salt mine at Baichengin Xinjiang Province, China Solarsalt Co. facility located on Bonaire, Netherlands Antilles Hyprsaline soil located near Alicante in southern Spain Open in a separate window + positive, − negative, nd not determined, T type strain DSM Deutsche Sammlung von Mikroorganismen, JCM Japan Collection of Microorganisms, ATCC American Type Culture Collection The differential phenotypic characteristics of the strain Cnaph3 and other related strains of the genus Halomonas

Techniques: Staining

Journal:

Article Title: Genetic Variability and Prevalence of Bartonella henselae in Cats in Berlin, Germany, and Analysis of Its Genetic Relatedness to a Strain from Berlin That Is Pathogenic for Humans

doi: 10.1128/JCM.39.2.743-746.2001

Figure Lengend Snippet: Characteristics of the B. henselae isolates studied

Article Snippet: These findings are in accordance with the data of Sander et al. ( 17 ), who found one type I isolate (5.9%) among 17 feline blood culture isolates in Freiburg, Germany. table ft1 table-wrap mode="anchored" t5 caption a7 Lane in Fig. A Isolate Clinical source 16S RNA type PFGE type 2 ATCC 49793 HIV patient (blood) I A 3 Houston-1 HIV patient (blood) I B 4 Berlin-1 HIV patient (BA-lesion) I B 5 Berlin-2 Cat (CSD a ) II C 5 6 Ber-K56 Cat II C 1 7 Ber-K111 Cat II C 1 8 Ber-K118 Cat II C 4 9 Ber-K132 Cat II C 1 10 Ber-K135 Cat II C 2 11 Ber-K181 Cat II C 2 12 Ber-K182 Cat II C 1 13 Ber-K169 Cat II C 3 14 Ber-K108 Cat II D 1 15 Ber-K116 Cat II D 1 16 Ber-K158 Cat II D 2 17 Ber-K156 Cat II E 1 18 Ber-K161 Cat II E 2 19 Ber-K123 Cat II F 20 Ber-K172 Cat II G 21 Ber-K186 Cat I F 22 Ber-K143 Cat II H 23 Ber-K153 Cat II I 24 Ber-K193 Cat II G Open in a separate window a Blood culture isolate from the pet cat of a patient with CSD.

Techniques:

Journal:

Article Title: Genetic Variability and Prevalence of Bartonella henselae in Cats in Berlin, Germany, and Analysis of Its Genetic Relatedness to a Strain from Berlin That Is Pathogenic for Humans

doi: 10.1128/JCM.39.2.743-746.2001

Figure Lengend Snippet: (A) DNA fingerprint analysis of the feline and human-pathogenic B. henselae isolates and the B. elizabethae strain ATCC 49927 by PFGE. (B) Dendrogram of the fingerprints as determined by unweighted pair group using mathematical averages. Lane 1, lambda ladder; lanes 2 to 4, human-pathogenic B. henselae isolates ATCC 49793 (Oklahoma), Houston-1, and Berlin-1, respectively; lanes 5 to 24, feline blood culture isolates of B. henselae; lane 25, B. elizabethae; lane 26, Saccharomyces cerevisiae chromosome marker.

Article Snippet: These findings are in accordance with the data of Sander et al. ( 17 ), who found one type I isolate (5.9%) among 17 feline blood culture isolates in Freiburg, Germany. table ft1 table-wrap mode="anchored" t5 caption a7 Lane in Fig. A Isolate Clinical source 16S RNA type PFGE type 2 ATCC 49793 HIV patient (blood) I A 3 Houston-1 HIV patient (blood) I B 4 Berlin-1 HIV patient (BA-lesion) I B 5 Berlin-2 Cat (CSD a ) II C 5 6 Ber-K56 Cat II C 1 7 Ber-K111 Cat II C 1 8 Ber-K118 Cat II C 4 9 Ber-K132 Cat II C 1 10 Ber-K135 Cat II C 2 11 Ber-K181 Cat II C 2 12 Ber-K182 Cat II C 1 13 Ber-K169 Cat II C 3 14 Ber-K108 Cat II D 1 15 Ber-K116 Cat II D 1 16 Ber-K158 Cat II D 2 17 Ber-K156 Cat II E 1 18 Ber-K161 Cat II E 2 19 Ber-K123 Cat II F 20 Ber-K172 Cat II G 21 Ber-K186 Cat I F 22 Ber-K143 Cat II H 23 Ber-K153 Cat II I 24 Ber-K193 Cat II G Open in a separate window a Blood culture isolate from the pet cat of a patient with CSD.

Techniques: Marker