Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: Comparison of molecular pathological markers in luteinized thecoma associated with sclerosing peritonitis and thecoma.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Comparison

Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: Molecular pathological markers show significant ascendant expression in LTSP. (A–F) Immunohistochemical staining of alpha-1,6-mannosylglycoprotein 6-beta- n -acetylglucosaminyltransferase B (MGAT5B), nuclear receptor coactivator 3 (NCOA3) and proliferation marker protein Ki-67 (MKI67) are respectively (A, C, E) diffusely positive in the luteinized cells but (B, D, F) weak to moderate in the thecoma cells. (G) Statistical analysis of the 3 markers. Data are expressed as mean ± SD. n = 9 to 11 sections per marker (G, LTSP); n = 81 to 85 sections per marker (G, Thecoma). *** P < .001 ( t test). Scale bar, 500 μm. AOD = average optical density, LTSP = luteinized thecoma associated with sclerosing peritonitis.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Expressing, Immunohistochemical staining, Staining, Marker

Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: MGAT5B-NCOA3 fusion gene in LTSP of the ovary. Schematic representation of the MGAT5B-NCOA3 fusion transcript including the exons and bases sequence involved. The breakpoint of the 5′ and 3′ partner genes are represented as red dotted lines. LTSP = luteinized thecoma associated with sclerosing peritonitis, MGAT5B = alpha-1,6-mannosylglycoprotein 6-beta- n -acetylglucosaminyltransferase B, NCOA3 = nuclear receptor coactivator 3.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Sequencing

Journal: Medicine

Article Title: Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

doi: 10.1097/MD.0000000000033911

Figure Lengend Snippet: MGAT5B-NCOA3 fusion gene is most common in LTSP. (A–C) MGAT5B-NCOA3 fusion gene is most common in (A) luteinized cells, but (B) rare in thecoma cells and (C) normal fimbriae tubae cells as assessed by FISH. FISH showing fusion signals for MGAT5B-NCOA3 with the overlapping yellow fluorescent signal (white arrows: orange, NCOA3; green, MGAT5B; yellow, fusion area). (D) Statistical analysis of positive incidence. Data are expressed as mean ± SD percentage. n = 7 cases (D, LTSP); n = 5 cases (D, Thecoma); n = 2 cases (D, Control). *** P < .001 (one-way analysis of variance and post hoc test). Scale bar, 250 μm. FISH = fluorescence in situ hybridization, LTSP = luteinized thecoma associated with sclerosing peritonitis, MGAT5B = alpha-1,6-mannosylglycoprotein 6-beta- n -acetylglucosaminyltransferase B, NCOA3 = nuclear receptor coactivator 3, ns = no significance.

Article Snippet: The tissue sections were stained for MGAT5B (1:200; catalog number 16993; Proteintech, Wuhan, China), NCOA3 (1:50, catalog number 20032, Proteintech), Vimentin (1:200, catalog number 10366, Proteintech), Catenin beta-1 (β-Catenin; 1:200, catalog number 17565, Proteintech), receptor tyrosine-protein kinase erbB-2 (HER2; 1:600, catalog number 18299, Proteintech), proliferation marker protein Ki-67 (MKI67; 1:5000, catalog number 27309, Proteintech), estrogen receptor (ESR; 1:200, catalog number 21244, Proteintech), progesterone receptor (PGR; 1:200, catalog number 25871, Proteintech), CD99 antigen (CD99; 1:200; catalog number abs136282; Absin, Shanghai, China), Wilms tumor protein (WT1; 1:200, catalog number abs135851, Absin), and hematoxylin-eosin as follows.

Techniques: Control, Fluorescence, In Situ Hybridization

Journal: Scientific Reports

Article Title: The clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production through mitochondrial quality control

doi: 10.1038/s41598-024-52582-7

Figure Lengend Snippet: Mitochondria are involved in LPS-induced IL-8 production. ( A ) Co-immunofluorescence imaging of NIPSNAPs (NIP1, 2, and 3; green) and HSP60 (a mitochondrial protein, red) or p62 (red). FLAG-tagged NIPSNAP1-, 2-, and 3-expressing BEAS-2B cells were co-stained with the indicated antibodies. The right columns are enlargements of the boxed regions in the left columns. Arrows in the right columns indicate colocalization of NIPSNAPs and p62. White scale bars in the left columns indicate 10 μm. ( B ) Detection of p62 interacting with NIPSNAPs in BEAS-2B cells by immunoprecipitation and WB (cropped). EGFP-expressing BEAS-2B cells were used as a negative control. IP and WCL indicate immunoprecipitate and whole-cell lysate, respectively. The numbers on the right indicate the molecular weights (kDa) of protein standards. ( C and D ) Detection of p62 by WB ( C : cropped) and the normalized IL-8 level in the culture supernatant ( D ) of p62 KD BEAS-2B cells stimulated with or without 100 ng/mL LPS for 6 h. Bars show the mean ± SD of four independent experiments. N.S., not significant, Student's t-test. ( E – G ) BEAS-2B ( E and G ) or H1-HeLa ( F ) cells were incubated with DFP (1 mM) or CCCP (20 μM) in the presence of 20 μM Q-VD-OPH, a caspase inhibitor. After incubation for 16 h, mtDNA ( E ) and the indicated proteins ( F ) were detected by quantitative PCR and WB (cropped), respectively. Relative expression levels of p62 and HSP60 normalized by the expression of β-actin are indicated under the WB images. ( G ) Normalized IL-8 levels (top) and induction rates (bottom) upon treatment with 100 ng/mL LPS for 6 h were quantified by an ELISA. Bars show the mean ± SD of three (for mtDNA measurement) and four (for IL-8 measurement) independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001; Student's t-test. Note that WB images were cropped to remove irrelevant areas, and the original images are shown in supplemental Fig. .

Article Snippet: CCCP (304-16993) and Q-VD-OPH (S7311) were obtained from FUJIFILM Wako Pure Chemical Corp. (FUJIFILM Wako, Osaka, Japan) and Selleck Chemicals (Houston, TX, USA), respectively, and dissolved in dimethyl sulfoxide.

Techniques: Immunofluorescence, Imaging, Expressing, Staining, Immunoprecipitation, Negative Control, Incubation, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay