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Image Search Results
Journal: Reproduction, Fertility and Development
Article Title: Microarray analysis of mRNA from cumulus cells following in vivo or in vitro maturation of mouse cumulus–oocyte complexes
doi: 10.1071/rd11305
Figure Lengend Snippet: Fig. 4. Western blot analysis of cumulus cells collected from 100 in vivo- matured (IVV) cumulus–oocyte complexes (COCs) or 100 in vitro-matured (IVM) COCs probed with an antibody to (a) haemoglobin A1 (HbA1) or (b) b-actin (ACTB). Protein from mouse heart (40 mg) was included as a positive control for HbA1.
Article Snippet: Membranes were blocked for 2 h at room temperature with 5% (w/v) skim milk, 1 TBST (10mM TrisHCl, pH 8.0, 150mMNaCl and 0.05%Tween 20) and incubated overnight at 48C with a rabbit monoclonal antibody specific for
Techniques: Western Blot, In Vivo, In Vitro, Positive Control