Journal: Molecules

Article Title: In Vitro Evaluation of Antimicrobial Peptide Tridecaptin M in Combination with Other Antibiotics against Multidrug Resistant Acinetobacter baumannii

doi: 10.3390/molecules25143255

Figure Lengend Snippet: Synergetic activity of rifampicin and vancomycin with tridecaptin M against Gram-negative bacteria.

Article Snippet: A. baumannii ATCC 19606 , Rifampicin , 4 , 1 , 0.25 , 16 , 0.31.

Techniques: Activity Assay, Bacteria, Concentration Assay

Journal: Molecules

Article Title: In Vitro Evaluation of Antimicrobial Peptide Tridecaptin M in Combination with Other Antibiotics against Multidrug Resistant Acinetobacter baumannii

doi: 10.3390/molecules25143255

Figure Lengend Snippet: Potentiation of various antibiotics in the presence of tridecaptin M in different A. baumannii strains.

Article Snippet: A. baumannii ATCC 19606 , Rifampicin , 4 , 1 , 0.25 , 16 , 0.31.

Techniques: Concentration Assay

Journal: Molecules

Article Title: In Vitro Evaluation of Antimicrobial Peptide Tridecaptin M in Combination with Other Antibiotics against Multidrug Resistant Acinetobacter baumannii

doi: 10.3390/molecules25143255

Figure Lengend Snippet: In vitro efficacy of tridecaptin M and rifampicin combination against A. baumannii ATCC 19606. ( a ) Killing of bacteria in nutrient-rich medium in the presence of antibiotics alone or in combination. The data are plotted as the mean of three replicates. Bars indicate the standard deviation. The experiment is representative of two biological repeats. ( b ) Killing of bacteria in rabbit blood after 4 h treatment. The data represent mean values of three replicates with standard deviation. The dotted line represents the limit of detection.

Article Snippet: A. baumannii ATCC 19606 , Rifampicin , 4 , 1 , 0.25 , 16 , 0.31.

Techniques: In Vitro, Bacteria, Standard Deviation

Journal: Molecules

Article Title: In Vitro Evaluation of Antimicrobial Peptide Tridecaptin M in Combination with Other Antibiotics against Multidrug Resistant Acinetobacter baumannii

doi: 10.3390/molecules25143255

Figure Lengend Snippet: Time-lag kinetics of tridecaptin M and rifampicin combination to study the effect on bacterial killing. ( a ) The late-exponential phase bacteria were exposed with rifampicin or tridecaptin M alone and in combination. In the combination panel, both antibiotics were added at the same time. The significance was calculated using the paired Student’s t -test with two-tailed distribution, *** denotes p < 0.0001. ( b ) The bacteria were initially incubated with rifampicin, and then tridecaptin M was added. ( c , d ) The bacteria were pre-treated with tridecaptin M for 2 h ( c ) or 4 h ( d ), and then tridecaptin was removed by centrifugation followed by the addition of rifampicin. In all the experiments, the data plotted are mean values of three replicates, and bars denote the standard deviation. The experiments are representatives of two biological replicates. In the graphs, the pretreatment bar denotes the initial CFU load before treatment with any of the antibiotics.

Article Snippet: A. baumannii ATCC 19606 , Rifampicin , 4 , 1 , 0.25 , 16 , 0.31.

Techniques: Bacteria, Two Tailed Test, Incubation, Centrifugation, Standard Deviation

Journal: Molecules

Article Title: In Vitro Evaluation of Antimicrobial Peptide Tridecaptin M in Combination with Other Antibiotics against Multidrug Resistant Acinetobacter baumannii

doi: 10.3390/molecules25143255

Figure Lengend Snippet: Effect of combination therapy on persisters’ survival. ( a ) The survival of bacterial persisters after 4 h treatment with rifampicin or tridecaptin M alone and in combination. The persisters were confirmed by determining the minimum inhibitory concentration (MIC) of two representatives after subculturing on a fresh agar plate containing no antibiotics. The MIC values were similar, indicating that persisters were not resistant to antibiotics. ( b ) NPN assay with stationary-phase bacteria indicating the permeabilization of persisters with tridecaptin M at different concentrations. Data plotted are mean values of three replicates. Bars denote the standard deviation.

Article Snippet: A. baumannii ATCC 19606 , Rifampicin , 4 , 1 , 0.25 , 16 , 0.31.

Techniques: Concentration Assay, Subculturing Assay, NPN Assay, Bacteria, Standard Deviation

Journal: BMC Microbiology

Article Title: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA

doi: 10.1186/1471-2180-10-72

Figure Lengend Snippet: Non- Francisella organisms from the strain collection of the Bundeswehr Institute of Microbiology tested to assess the specificity of the FISH assay

Article Snippet: Pasteurella multocida , DSMZ 5281 , + , - , -.

Techniques: