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Image Search Results
Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
Article Title: Identification of Novel Genes Selectively Expressed in the Follicle-Associated Epithelium from the Meta-Analysis of Transcriptomics Data from Multiple Mouse Cell and Tissue Populations
doi: 10.1093/dnares/dss022
Figure Lengend Snippet: KCNJ15 is expressed throughout the FAE, but not by M cells or the villous epithelium. (A) The Affymetrix probe sets registered for Kcnj15 which were co-expressed within cluster 65 were surveyed with the RefDIC probe-mapping tool to confirm that they recognized exons within this gene (red bars). NM_019664 and NM_001039056 accession numbers of reference sequences. (B) Heat map showing the expression of profile throughout the intestine of probe sets encoding KCNJ potassium channels (a representative probe set is shown when multiple probe sets for a gene were present on the array). Each column represents the mean probe set intensity (log 2 ) for all samples from each source ( n = 2–4). Kcnj15 was the only gene which was expressed significantly within the FAE at levels >2.0-fold when compared with the villous epithelium. The red-boxed area indicates the mAb NKM 16-2-4-enriched Peyer's patch M cell data sets; the blue-boxed area indicates the FAE data sets. (C) IHC analysis of mouse Peyer's patches shows the cytoplasmic expression of KCNJ15 (red) throughout cells within the FAE, but not in M cells (GP2 + cells, green, arrows) or the villous epithelium. SED, subepithelial dome. Dotted line indicates the border of the FAE.
Article Snippet: Serial frozen sections (10 μm in thickness) of Peyer's patches were cut on a cryostat and immunostained with the following antibodies: rat anti-mouse GP2 monoclonal antibody (mAb; MBL International, Woburn, MA, USA) or rabbit anti-annexin-A5 (ANXA5) polyclonal antibody (pAb; Abcam, Cambridge, UK) to detect M cells, goat anti-mouse CCL20 pAb (R&D Systems, Abingdon, UK), Alexa Fluor 488-conjugated hamster anti-mouse CD11c mAb (clone N418; eBioscience, Hatfield, UK), biotinylated rat anti-mouse CD68 mAb (clone FA-11; AbD Serotec, Oxford, UK), biotinylated rat anti-E-cadherin (clone DECMA-1; eBioscience),
Techniques: Expressing