15938 Search Results


b pseudomallei atcc 23343t  (ATCC)
90
ATCC b pseudomallei atcc 23343t
B Pseudomallei Atcc 23343t, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b pseudomallei atcc 23343t/product/ATCC
Average 90 stars, based on 1 article reviews
b pseudomallei atcc 23343t - by Bioz Stars, 2026-03
90/100 stars
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endogenous cnot10  (Proteintech)
93
Proteintech endogenous cnot10
Figure 1. Structure of the N-terminal module of the human CCR4-NOT complex: A structured core with an antenna domain (A) Coomassie-stained SDS-PAGE analysis of the purified recombinant sample of the human <t>CNOT1N-CNOT10-CNOT11</t> complex used for crystal structure determination. The complex corresponds to the N-terminal module of CCR4-NOT and includes only the N-terminal region of the scaffold protein CNOT1N. (B) Schematic representation of the domain organization of human CNOT1N (yellow), CNOT10 (light green), and CNOT11 (blue). The structured domains are indicated as rectangles and linker regions are shown as black lines. Labeling of the different portions of the proteins corresponds to the structural analysis, as discussed in the text. In particular, the N-terminal, middle, and C-terminal region of CNOT11 are indicated with the corresponding suffix N, M, C. HEAT,35 TPR,36
Endogenous Cnot10, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous cnot10/product/Proteintech
Average 93 stars, based on 1 article reviews
endogenous cnot10 - by Bioz Stars, 2026-03
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pcdna3 htra2 flag  (Addgene inc)
91
Addgene inc pcdna3 htra2 flag
Figure 1. Structure of the N-terminal module of the human CCR4-NOT complex: A structured core with an antenna domain (A) Coomassie-stained SDS-PAGE analysis of the purified recombinant sample of the human <t>CNOT1N-CNOT10-CNOT11</t> complex used for crystal structure determination. The complex corresponds to the N-terminal module of CCR4-NOT and includes only the N-terminal region of the scaffold protein CNOT1N. (B) Schematic representation of the domain organization of human CNOT1N (yellow), CNOT10 (light green), and CNOT11 (blue). The structured domains are indicated as rectangles and linker regions are shown as black lines. Labeling of the different portions of the proteins corresponds to the structural analysis, as discussed in the text. In particular, the N-terminal, middle, and C-terminal region of CNOT11 are indicated with the corresponding suffix N, M, C. HEAT,35 TPR,36
Pcdna3 Htra2 Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 htra2 flag/product/Addgene inc
Average 91 stars, based on 1 article reviews
pcdna3 htra2 flag - by Bioz Stars, 2026-03
91/100 stars
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Image Search Results


Figure 1. Structure of the N-terminal module of the human CCR4-NOT complex: A structured core with an antenna domain (A) Coomassie-stained SDS-PAGE analysis of the purified recombinant sample of the human CNOT1N-CNOT10-CNOT11 complex used for crystal structure determination. The complex corresponds to the N-terminal module of CCR4-NOT and includes only the N-terminal region of the scaffold protein CNOT1N. (B) Schematic representation of the domain organization of human CNOT1N (yellow), CNOT10 (light green), and CNOT11 (blue). The structured domains are indicated as rectangles and linker regions are shown as black lines. Labeling of the different portions of the proteins corresponds to the structural analysis, as discussed in the text. In particular, the N-terminal, middle, and C-terminal region of CNOT11 are indicated with the corresponding suffix N, M, C. HEAT,35 TPR,36

Journal: Cell reports

Article Title: The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions.

doi: 10.1016/j.celrep.2022.111902

Figure Lengend Snippet: Figure 1. Structure of the N-terminal module of the human CCR4-NOT complex: A structured core with an antenna domain (A) Coomassie-stained SDS-PAGE analysis of the purified recombinant sample of the human CNOT1N-CNOT10-CNOT11 complex used for crystal structure determination. The complex corresponds to the N-terminal module of CCR4-NOT and includes only the N-terminal region of the scaffold protein CNOT1N. (B) Schematic representation of the domain organization of human CNOT1N (yellow), CNOT10 (light green), and CNOT11 (blue). The structured domains are indicated as rectangles and linker regions are shown as black lines. Labeling of the different portions of the proteins corresponds to the structural analysis, as discussed in the text. In particular, the N-terminal, middle, and C-terminal region of CNOT11 are indicated with the corresponding suffix N, M, C. HEAT,35 TPR,36

Article Snippet: GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/1000 or 1/5000 dilution, endogenous CNOT10, CNOT7 and CNOT11 proteins were revealed with rabbit polyclonal antiCNOT10 antibody (15938-1- AP, Proteintech) used at 1/1000 dilution or with home-made rabbit anti-CNOT7 or anti-CNOT11 antiserum used at 1/1000 dilution, respectively.

Techniques: Staining, SDS Page, Recombinant, Labeling

Figure 2. The structured core of the CNOT1N-CNOT10-CNOT11 complex is formed by evolutionary conserved interactions (A–F) Zoom-in views of the major structural interactions in the CNOT1N-CNOT10-CNOT11N-M core of the CCR4-NOT N-terminal module. The individual views are also indicated in the context of the entire structure. (A–C) highlight conserved interactions within the inner layer of the complex comprising the extended CNOT11M region and the CNOT10 TPR superhelix. The interactions with the two outer layers are shown in (D and E) (CNOT1N domain 1, CNOT11, and CNOT10) and (F) (CNOT1N domain 2 and CNOT10). The evolutionary conservation of the interactions is shown in Figures S2A–S2C. (G) Biochemical validation of the structural analysis. Co-immunoprecipitation of endogenous CNOT10 with GFP-tagged CNOT11 truncated proteins. HEK293 cells were transfected with plasmids expressing GFP-tagged human CNOT11 fusion proteins or empty GFP expression vector. Proteins were immunoprecip- itated with GFP-Trap magnetic beads and the co-precipitation was analyzed by western blotting. For some constructs, limited CNOT11 degradation during immunoprecipitation generated additional lower molecular-weight bands.

Journal: Cell reports

Article Title: The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions.

doi: 10.1016/j.celrep.2022.111902

Figure Lengend Snippet: Figure 2. The structured core of the CNOT1N-CNOT10-CNOT11 complex is formed by evolutionary conserved interactions (A–F) Zoom-in views of the major structural interactions in the CNOT1N-CNOT10-CNOT11N-M core of the CCR4-NOT N-terminal module. The individual views are also indicated in the context of the entire structure. (A–C) highlight conserved interactions within the inner layer of the complex comprising the extended CNOT11M region and the CNOT10 TPR superhelix. The interactions with the two outer layers are shown in (D and E) (CNOT1N domain 1, CNOT11, and CNOT10) and (F) (CNOT1N domain 2 and CNOT10). The evolutionary conservation of the interactions is shown in Figures S2A–S2C. (G) Biochemical validation of the structural analysis. Co-immunoprecipitation of endogenous CNOT10 with GFP-tagged CNOT11 truncated proteins. HEK293 cells were transfected with plasmids expressing GFP-tagged human CNOT11 fusion proteins or empty GFP expression vector. Proteins were immunoprecip- itated with GFP-Trap magnetic beads and the co-precipitation was analyzed by western blotting. For some constructs, limited CNOT11 degradation during immunoprecipitation generated additional lower molecular-weight bands.

Article Snippet: GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/1000 or 1/5000 dilution, endogenous CNOT10, CNOT7 and CNOT11 proteins were revealed with rabbit polyclonal antiCNOT10 antibody (15938-1- AP, Proteintech) used at 1/1000 dilution or with home-made rabbit anti-CNOT7 or anti-CNOT11 antiserum used at 1/1000 dilution, respectively.

Techniques: Biomarker Discovery, Immunoprecipitation, Transfection, Expressing, Plasmid Preparation, Magnetic Beads, Western Blot, Construct, Generated, Molecular Weight