Journal: Molecular cell

Article Title: Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity

doi: 10.1016/j.molcel.2023.01.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following kinase inhibitors were used as KIPs for competition experiments at the given final concentrations: GSK-690693 (10 μM, MedChemExpress, MCE, Monmouth Junction, NJ), Miliciclib (10 μM, MCE), Rebastinib (10 μM, MCE), AT9283 (10 μM, MCE), TAK-901 (10 μM, MCE), RGB-286638 (10 μM, MCE), Flavopiridol*HCl (10 μM, MCE), PF-562271 besylate (10 μM, MCE), Dabrafenib mesylate (10 μM, MCE), OTSSP167*HCl (10 μM, MCE), CYC-116 (10 μM, MCE), Silmitasertib (10 μM, MCE), SB1317 (10 μM, MCE), XL228 (10 μM, MCE), Sapanisertib (10 μM, MCE), PF-3758309 (10 μM, ApexBio), Staurosporine (replacing the structurally closely related K-252a, 1 μM, LC Labs, Woburn, MA), AZD-7762 (10 μM Selleckchem), Bosutinib (10 μM, Selleckchem), Dasatinib (10 μM, Selleckchem), LP-935509 (1 μM, MCE), and Linsitinib (10 μM, ApexBio). . Kinobead/LC-MS competition with the 21 KIPs and formaldehyde-mediated protein crosslinking Kinobead competition with formaldehyde-mediated protein crosslinking was performed as described in ‘Kinase affinity enrichment, KI competition, and on-bead digestion’ above with the following modifications.

Techniques: Virus, Recombinant, Mass Spectrometry, Protease Inhibitor, Western Blot, shRNA, Plasmid Preparation, Software, Real-time Polymerase Chain Reaction