Journal: Journal of Bacteriology

Article Title: Mlc of Thermus thermophilus : a Glucose-Specific Regulator for a Glucose/Mannose ABC Transporter in the Absence of the Phosphotransferase System

doi: 10.1128/jb.00715-06

Figure Lengend Snippet: FIG. 4. Mlc glucose/mannose transport operon and TMSP ABC transport operon organization in T. thermophilus. The TTC numbers refer to the gene numbering in the genome sequencing of T. thermophilus HB27 (10). We propose the names GlcE, GlcF, and GlcG for the glucose/ mannose-binding protein and the two membrane components of the glucose/mannose ABC transporter.

Article Snippet: Strains and plasmids used in this study Strain or plasmid Known genotypea Source orreference Strains E. coli SF120 ptr32::cm degP4::kan ompT 2 JM-G2 ptsG-lacZ mlc::Tn10 22 T. thermophilus DSM7029 Wild-type strain (HB27) DSMZ CL3 mlc::kan This study HB27 malK1::kan malK1::kan 28 JN1 malF::bleo Jutta Nesper CL4 mlc::kan malF::bleo This study Plasmids pFC4 pGDR11 lacIq; N-terminally His6-tagged MlcTth (gene TTC0329); Amp r This study pEM1 pCS19 lacIq; wild-type MlcTth (gene TTC0329); Amp r This study pCL2 pQE30 (QIAGEN) lacIq; N-terminally His6-tagged Mlc916Tth::Kan; Amp r Kanr This study pTTC0328 pQE30 (QIAGEN) lacIq; N-terminally His6-tagged glucose-binding protein (gene TTC0328) without its signal sequence This study pCL10 pQE30(QIAGEN) lacIq; N-terminal His6-tagged TMSP transporter binding protein (gene TTC1627) without its signal sequence This study pCS19 pQE60 (QIAGEN) lacIq; Ampr 29 pGDR11 pQE31 (QIAGEN) lacIq; N-terminal His6 tag; Amp r 20 pMK18 pUC18 derivative with a thermostable resistance to kanamycin 6 pREP4 pACYC derivative containing the p15A replicon; Kanr QIAGEN on M arch 7, 2015 by M A H ID O L U N IV F A C O F M E D http://jb.asm .org/ D ow nloaded from ampicillin/ml and 25 g of kanamycin/ml.

Techniques: Sequencing, Binding Assay, Membrane

Journal: Journal of Bacteriology

Article Title: Mlc of Thermus thermophilus : a Glucose-Specific Regulator for a Glucose/Mannose ABC Transporter in the Absence of the Phosphotransferase System

doi: 10.1128/jb.00715-06

Figure Lengend Snippet: FIG. 7. Glucose transport kinetics of T. thermophilus. (A) Wild type (HB27); (B) malF::bleo mutant (JN1); (C) mlc::kan mutant (CL3). Transport by JN1 represents the activity of the glucose/man- nose ABC transporter, and transport by CL3 represents the activity of the TMSP ABC transporter. The curves can be fitted according to the Michaelis-Menten equation and yield a Km of 0.15 M for glucose uptake via the glucose/mannose transporter and a Km of 1.4 M for the TMSP transporter. A double mutant harboring both malF::bleo and mlc::kan exhibits neither glucose nor maltose transport activity. TABLE 2. Transport of glucose and maltose in T. thermophilus

Article Snippet: Strains and plasmids used in this study Strain or plasmid Known genotypea Source orreference Strains E. coli SF120 ptr32::cm degP4::kan ompT 2 JM-G2 ptsG-lacZ mlc::Tn10 22 T. thermophilus DSM7029 Wild-type strain (HB27) DSMZ CL3 mlc::kan This study HB27 malK1::kan malK1::kan 28 JN1 malF::bleo Jutta Nesper CL4 mlc::kan malF::bleo This study Plasmids pFC4 pGDR11 lacIq; N-terminally His6-tagged MlcTth (gene TTC0329); Amp r This study pEM1 pCS19 lacIq; wild-type MlcTth (gene TTC0329); Amp r This study pCL2 pQE30 (QIAGEN) lacIq; N-terminally His6-tagged Mlc916Tth::Kan; Amp r Kanr This study pTTC0328 pQE30 (QIAGEN) lacIq; N-terminally His6-tagged glucose-binding protein (gene TTC0328) without its signal sequence This study pCL10 pQE30(QIAGEN) lacIq; N-terminal His6-tagged TMSP transporter binding protein (gene TTC1627) without its signal sequence This study pCS19 pQE60 (QIAGEN) lacIq; Ampr 29 pGDR11 pQE31 (QIAGEN) lacIq; N-terminal His6 tag; Amp r 20 pMK18 pUC18 derivative with a thermostable resistance to kanamycin 6 pREP4 pACYC derivative containing the p15A replicon; Kanr QIAGEN on M arch 7, 2015 by M A H ID O L U N IV F A C O F M E D http://jb.asm .org/ D ow nloaded from ampicillin/ml and 25 g of kanamycin/ml.

Techniques: Mutagenesis, Activity Assay

Journal: Biotechnology for Biofuels

Article Title: Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum

doi: 10.1186/s13068-015-0269-0

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: The plasmid construction was carried out with PCR fragments (KOD, Novagen, Darmstadt, Germany) generated with genomic DNA of C. glutamicum WT, E. coli DH5α (DNA preparation described by [ ]), or K. oxytoca DSM4798 (DSMZ, Braunschweig, Germany) as template DNA.

Techniques: Plasmid Preparation, Gene Expression, Construct, Derivative Assay, Over Expression, Binding Assay

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: Haloferax mediterranei DSM1411 Cell Dry Mass (CDM) and PHBV production (panel A ) and yield (panel B ) in different fermentation conditions. R-NF was supplemented with 1% ( v / v ) SL-6 and 10% ( w / v ) NaCl. Inoculum was made with 10 (I10) or 20% v / v (I20) active cultures of Haloferax mediterranei DSM1411 grown (24 h at 37 °C) in HM372 (I10-I20 372 ) or HM97 (I10-I20 97 ) media. Fermentation was carried out at 37 °C for all the thesis, with the exception of I20 372 45, incubated at 45 °C. 10% w / v NaCl (final concentration 20%) or 5 g/L YE were added to the substrate inoculated with 20% of active culture in HM372 (I20 372 NaCl and I20 372 YE). In all cases, fermentation lasted 120 h. Error bars represent the standard deviation of three replicates. a–d Values in the same panel and data series, with different superscript letters, differ significantly ( p < 0.05).

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Incubation, Concentration Assay, Standard Deviation

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: Haloferax mediterranei Cell Dry Mass (CDM) and PHBV production in R-NF supplemented with 1% ( v / v ) SL-6 and 10% ( w / v ) NaCl. Inoculum was made with 20% v / v active culture of Hfx. mediterranei DSM1411 grown (24 h at 37 °C) in HM372. Fermentation was carried out at 37 °C for 144 h. Error bars represent the standard deviation of three replicates. a–c Values in the same data series, with different superscript letters, differ significantly ( p < 0.05).

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Standard Deviation

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: DSC thermograms (−20–200 °C, panel A ; −20–20 °C, panel B ) obtained from the second heating of the PHBV purified from R-NF fermented with Haloferax mediterranei DSM1411 (EXP) and the commercial reference ENMAT™ Y1000P (ENMAT).

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Purification

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: Thermal properties of the PHBV purified from R-NF fermented with Hfx. mediterranei DSM1411 (EXP) and the commercial reference ENMAT™ Y1000P, as obtained by the differential scanning calorimetry (DSC) analysis. T g , glass transition temperature; T c , crystallization temperature; T m , melting temperature; ΔH m and ΔH m , melting and crystallization enthalpies; X crystallinity degree.

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Purification, Differential Scanning Calorimetry, Crystallization Assay

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectra of the PHBV purified from R-NF fermented with Haloferax mediterranei DSM1411 (panel A ) and of the commercial reference ENMAT™ Y1000P ( B ).

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Fourier Transform Infrared Spectroscopy, Purification

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: Absorption peaks of the PHBV purified from R-NF fermented with Hfx. mediterranei DSM1411.

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Purification, Functional Assay

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: Atomic percentages, based on the spectral analysis, of the surface of PHBV film purified from R-NF fermented with Hfx. mediterranei DSM1411 (EXP) and of the commercial reference ENMAT™ Y1000P.

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Purification

Journal: Foods

Article Title: Production of the Polyhydroxyalkanoate PHBV from Ricotta Cheese Exhausted Whey by Haloferax mediterranei Fermentation

doi: 10.3390/foods9101459

Figure Lengend Snippet: Spectral components and relative abundance (%) of the surface of PHBV film purified from R-NF fermented with Hfx. mediterranei DSM1411 (EXP) and of the commercial reference ENMAT™ Y1000P, as obtained by XPS analysis.

Article Snippet: Haloferax mediterranei DSM1411 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen culture collection (DSMZ, Braunschweig, Germany) [ , ].

Techniques: Purification

Journal: Journal of Bacteriology

Article Title: Mlc of Thermus thermophilus : a Glucose-Specific Regulator for a Glucose/Mannose ABC Transporter in the Absence of the Phosphotransferase System

doi: 10.1128/jb.00715-06

Figure Lengend Snippet: FIG. 4. Mlc glucose/mannose transport operon and TMSP ABC transport operon organization in T. thermophilus. The TTC numbers refer to the gene numbering in the genome sequencing of T. thermophilus HB27 (10). We propose the names GlcE, GlcF, and GlcG for the glucose/ mannose-binding protein and the two membrane components of the glucose/mannose ABC transporter.

Article Snippet: T. thermophilus strain HB27 (DSM7039) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany.

Techniques: Sequencing, Binding Assay, Membrane

Journal: Journal of Bacteriology

Article Title: Mlc of Thermus thermophilus : a Glucose-Specific Regulator for a Glucose/Mannose ABC Transporter in the Absence of the Phosphotransferase System

doi: 10.1128/jb.00715-06

Figure Lengend Snippet: FIG. 7. Glucose transport kinetics of T. thermophilus. (A) Wild type (HB27); (B) malF::bleo mutant (JN1); (C) mlc::kan mutant (CL3). Transport by JN1 represents the activity of the glucose/man- nose ABC transporter, and transport by CL3 represents the activity of the TMSP ABC transporter. The curves can be fitted according to the Michaelis-Menten equation and yield a Km of 0.15 M for glucose uptake via the glucose/mannose transporter and a Km of 1.4 M for the TMSP transporter. A double mutant harboring both malF::bleo and mlc::kan exhibits neither glucose nor maltose transport activity. TABLE 2. Transport of glucose and maltose in T. thermophilus

Article Snippet: T. thermophilus strain HB27 (DSM7039) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany.

Techniques: Mutagenesis, Activity Assay