15218 Search Results


v stercoraria atcc 15218  (ATCC)
92
ATCC v stercoraria atcc 15218
V Stercoraria Atcc 15218, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chloroquine  (MedChemExpress)
96
MedChemExpress chloroquine
Chloroquine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti stip1 antibody  (Proteintech)
93
Proteintech rabbit anti stip1 antibody
(A–C) Degradation assay in immunofluorescence against Ca V 1.2 (A) and Ca V 2.3 (B) of primary beta cells of the indicated genotypes after the CHX treatment of the indicated periods; accompanied by their quantification along with that of α-tubulin (C). Bars, 5 μm. * p < 0.05, *** p < 0.001, Welch’s t test at the indicated time; n = 6 (Ca V 1.2), 17–24 (Ca V 2.3), 5–6 (α-tubulin). (D and E) Brefeldin-A (BFA) washout assay with an LSM 5LIVE-Duo microscope, assessing the speeds of post-Golgi trafficking of Ca V 1.2-EGFP proteins expressed in primary beta cells of the indicated genotypes (D), accompanied by its quantification (E). Time after BFA washout is indicated. Bar, 5 μm. *p < 0.05, Welch’s t test, n = 11. Arrows, the timing of plasma membrane fusion. Corresponding to Movie EV4. (F) TIRF/STORM microscopy of a wild-type primary mouse beta cell immunolabeled against Ca V 1.2 and KIF5B. Scale bar, 5 μm. Arrows, colocalizing spots. (G) TIRF/STORM microscopy of primary mouse beta cells of the indicated genotypes immunolabeled against Ca V 1.2 (green) and Ca V 2.3 (magenta). Scale bars, 5 μm. (H) Schematic representation of <t>STIP1-dependent</t> Hsp70-to-Hsp90 chaperone exchange machinery. (I and J) z -projection of proximity ligation assay in CT and cKO primary beta cells showing the protein binding between Ca V 1.2 and the indicated Hsp proteins (I); accompanied by quantification (J). ** p < 0.01; *** p < 0.001; Welch’s t test, n = 6. (K) Immunoblotting of scramble control (SC) and STIP1-knockdown (KD) MIN6 cells against the indicated epitopes. Note that STIP1 deficiency induced downregulation of Ca V 1.2 and BK Ca proteins. Reproduced twice.
Rabbit Anti Stip1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fccp cayman 15218  (Cayman Chemical)
90
Cayman Chemical fccp cayman 15218
(A–C) Degradation assay in immunofluorescence against Ca V 1.2 (A) and Ca V 2.3 (B) of primary beta cells of the indicated genotypes after the CHX treatment of the indicated periods; accompanied by their quantification along with that of α-tubulin (C). Bars, 5 μm. * p < 0.05, *** p < 0.001, Welch’s t test at the indicated time; n = 6 (Ca V 1.2), 17–24 (Ca V 2.3), 5–6 (α-tubulin). (D and E) Brefeldin-A (BFA) washout assay with an LSM 5LIVE-Duo microscope, assessing the speeds of post-Golgi trafficking of Ca V 1.2-EGFP proteins expressed in primary beta cells of the indicated genotypes (D), accompanied by its quantification (E). Time after BFA washout is indicated. Bar, 5 μm. *p < 0.05, Welch’s t test, n = 11. Arrows, the timing of plasma membrane fusion. Corresponding to Movie EV4. (F) TIRF/STORM microscopy of a wild-type primary mouse beta cell immunolabeled against Ca V 1.2 and KIF5B. Scale bar, 5 μm. Arrows, colocalizing spots. (G) TIRF/STORM microscopy of primary mouse beta cells of the indicated genotypes immunolabeled against Ca V 1.2 (green) and Ca V 2.3 (magenta). Scale bars, 5 μm. (H) Schematic representation of <t>STIP1-dependent</t> Hsp70-to-Hsp90 chaperone exchange machinery. (I and J) z -projection of proximity ligation assay in CT and cKO primary beta cells showing the protein binding between Ca V 1.2 and the indicated Hsp proteins (I); accompanied by quantification (J). ** p < 0.01; *** p < 0.001; Welch’s t test, n = 6. (K) Immunoblotting of scramble control (SC) and STIP1-knockdown (KD) MIN6 cells against the indicated epitopes. Note that STIP1 deficiency induced downregulation of Ca V 1.2 and BK Ca proteins. Reproduced twice.
Fccp Cayman 15218, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fccp cayman chemicals  (Cayman Chemical)
90
Cayman Chemical fccp cayman chemicals
Psychotropic drugs induce mitochondrial membrane depolarization. Mitochondrial membrane potential depolarization was evaluated by <t>JC-1</t> <t>staining</t> after overnight treatment with psychotropic compounds (5 μmol/L) in MCF7 cells. Pictures were acquired by fluorescence microscopy. Representative images of cell treated with the negative control DMSO, carbonyl cyanide 4-[trifluoromethoxy]phenylhydrazone <t>(FCCP),</t> positive control, ebastine, fluoxetine, fluspirilene, nefazodone, penfluridol, pimozide, and spiperone (A) . Histogram showing quantification of red/green fluorescent ratio as fold change relative to control (B) . Data are presented as mean ± SD from three independent experiments, each performed in triplicate. **, Student's T -test p < 0.01; ***, Student's T -test p < 0.001.
Fccp Cayman Chemicals, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jvac 15218  (bioCSL Inc)
90
bioCSL Inc jvac 15218
Psychotropic drugs induce mitochondrial membrane depolarization. Mitochondrial membrane potential depolarization was evaluated by <t>JC-1</t> <t>staining</t> after overnight treatment with psychotropic compounds (5 μmol/L) in MCF7 cells. Pictures were acquired by fluorescence microscopy. Representative images of cell treated with the negative control DMSO, carbonyl cyanide 4-[trifluoromethoxy]phenylhydrazone <t>(FCCP),</t> positive control, ebastine, fluoxetine, fluspirilene, nefazodone, penfluridol, pimozide, and spiperone (A) . Histogram showing quantification of red/green fluorescent ratio as fold change relative to control (B) . Data are presented as mean ± SD from three independent experiments, each performed in triplicate. **, Student's T -test p < 0.01; ***, Student's T -test p < 0.001.
Jvac 15218, supplied by bioCSL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fccp item no. 15218  (Cayman Chemical)
90
Cayman Chemical fccp item no. 15218
Psychotropic drugs induce mitochondrial membrane depolarization. Mitochondrial membrane potential depolarization was evaluated by <t>JC-1</t> <t>staining</t> after overnight treatment with psychotropic compounds (5 μmol/L) in MCF7 cells. Pictures were acquired by fluorescence microscopy. Representative images of cell treated with the negative control DMSO, carbonyl cyanide 4-[trifluoromethoxy]phenylhydrazone <t>(FCCP),</t> positive control, ebastine, fluoxetine, fluspirilene, nefazodone, penfluridol, pimozide, and spiperone (A) . Histogram showing quantification of red/green fluorescent ratio as fold change relative to control (B) . Data are presented as mean ± SD from three independent experiments, each performed in triplicate. **, Student's T -test p < 0.01; ***, Student's T -test p < 0.001.
Fccp Item No. 15218, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90/100 stars
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Image Search Results


(A–C) Degradation assay in immunofluorescence against Ca V 1.2 (A) and Ca V 2.3 (B) of primary beta cells of the indicated genotypes after the CHX treatment of the indicated periods; accompanied by their quantification along with that of α-tubulin (C). Bars, 5 μm. * p < 0.05, *** p < 0.001, Welch’s t test at the indicated time; n = 6 (Ca V 1.2), 17–24 (Ca V 2.3), 5–6 (α-tubulin). (D and E) Brefeldin-A (BFA) washout assay with an LSM 5LIVE-Duo microscope, assessing the speeds of post-Golgi trafficking of Ca V 1.2-EGFP proteins expressed in primary beta cells of the indicated genotypes (D), accompanied by its quantification (E). Time after BFA washout is indicated. Bar, 5 μm. *p < 0.05, Welch’s t test, n = 11. Arrows, the timing of plasma membrane fusion. Corresponding to Movie EV4. (F) TIRF/STORM microscopy of a wild-type primary mouse beta cell immunolabeled against Ca V 1.2 and KIF5B. Scale bar, 5 μm. Arrows, colocalizing spots. (G) TIRF/STORM microscopy of primary mouse beta cells of the indicated genotypes immunolabeled against Ca V 1.2 (green) and Ca V 2.3 (magenta). Scale bars, 5 μm. (H) Schematic representation of STIP1-dependent Hsp70-to-Hsp90 chaperone exchange machinery. (I and J) z -projection of proximity ligation assay in CT and cKO primary beta cells showing the protein binding between Ca V 1.2 and the indicated Hsp proteins (I); accompanied by quantification (J). ** p < 0.01; *** p < 0.001; Welch’s t test, n = 6. (K) Immunoblotting of scramble control (SC) and STIP1-knockdown (KD) MIN6 cells against the indicated epitopes. Note that STIP1 deficiency induced downregulation of Ca V 1.2 and BK Ca proteins. Reproduced twice.

Journal: bioRxiv

Article Title: Kinesin-1 mediates proper ER folding of the Ca V 1.2 channel and maintains mouse glucose homeostasis

doi: 10.1101/2024.06.24.600327

Figure Lengend Snippet: (A–C) Degradation assay in immunofluorescence against Ca V 1.2 (A) and Ca V 2.3 (B) of primary beta cells of the indicated genotypes after the CHX treatment of the indicated periods; accompanied by their quantification along with that of α-tubulin (C). Bars, 5 μm. * p < 0.05, *** p < 0.001, Welch’s t test at the indicated time; n = 6 (Ca V 1.2), 17–24 (Ca V 2.3), 5–6 (α-tubulin). (D and E) Brefeldin-A (BFA) washout assay with an LSM 5LIVE-Duo microscope, assessing the speeds of post-Golgi trafficking of Ca V 1.2-EGFP proteins expressed in primary beta cells of the indicated genotypes (D), accompanied by its quantification (E). Time after BFA washout is indicated. Bar, 5 μm. *p < 0.05, Welch’s t test, n = 11. Arrows, the timing of plasma membrane fusion. Corresponding to Movie EV4. (F) TIRF/STORM microscopy of a wild-type primary mouse beta cell immunolabeled against Ca V 1.2 and KIF5B. Scale bar, 5 μm. Arrows, colocalizing spots. (G) TIRF/STORM microscopy of primary mouse beta cells of the indicated genotypes immunolabeled against Ca V 1.2 (green) and Ca V 2.3 (magenta). Scale bars, 5 μm. (H) Schematic representation of STIP1-dependent Hsp70-to-Hsp90 chaperone exchange machinery. (I and J) z -projection of proximity ligation assay in CT and cKO primary beta cells showing the protein binding between Ca V 1.2 and the indicated Hsp proteins (I); accompanied by quantification (J). ** p < 0.01; *** p < 0.001; Welch’s t test, n = 6. (K) Immunoblotting of scramble control (SC) and STIP1-knockdown (KD) MIN6 cells against the indicated epitopes. Note that STIP1 deficiency induced downregulation of Ca V 1.2 and BK Ca proteins. Reproduced twice.

Article Snippet: A rabbit anti-Ca V 1.2 antibody (N-17-R, #sc-16229-R, RRID:AB_2228387), a rabbit anti-K ir 6.2 antibody (H-55; #sc-20809; RRID:AB_2130466), a goat anti-PIP5Kα (PIPKIα) antibody (M-20, #sc-11775; RRID:AB_2268303), and a goat anti-SUR1 antibody (N-18, #sc-11226; RRID:AB_2130475) were purchased from Santa Cruz Biotechnology; a mouse anti-PIP 2 IgM antibody (#Z-A045, RRID:AB_427211) was from Echelon Research labs; a rabbit anti-GFP antibody (#598, RRID:AB_591819) was from MBL; a mouse anti-LC3 antibody (Clone LC3-1703, #CTB-LC3-2-IC, RRID:AB_10707197) was from Cosmo Bio; a rabbit anti-BK Ca (K Ca 1.1) antibody (#APC-151, RRID:AB_10915895) and a rabbit anti-Ca V 2.3 antibody (#ACC-006, RRID:AB_2039777) were from Alomone Labs; a mouse anti-syntaxin-1 antibody (#MAB336, RRID:AB_2196527) was from Millipore; a mouse anti-Na/K ATPase beta 2 antibody (#610914; RRID:AB_398231) and a mouse anti-paxillin antibody (#610051, RRID:AB_397463) were from BD Transduction Labs; a mouse anti-Hsc70/Hsp70 antibody (Clone BB70, #ADI-SPA-822-D, RRID:AB_2039252) and a rat anti-Hsp90 antibody (Clone 16F1, #ADI-SPA-835-D, RRID:AB_2039281) were from Enzo; a rabbit anti-STIP1 antibody (#15218-1-AP; RRID:AB_2255518) and a rabbit anti-calnexin-1 (CANX) antibody (#10427-2-AP, RRID:AB_2069033) were from Proteintech; a mouse anti-derlin-1 antibody (#SAB4200148, RRID:AB_10624068), an anti-α-tubulin antibody (Clone DM1A; #CP06; RRID:AB_2617116), and an anti-insulin antibody (Clone K36aC10, #I-2018, Sigma Aldrich; RRID:AB_260137) were from Sigma Aldrich; a rabbit anti-phospho Src antibody for pSFK (#ab32078; RRID:AB_2286707) was from Epitomics/Abcam; and a rabbit anti-KIF5B antibody (RRID:AB_2571745) was previously described ( Tanaka et al ., 1998 ).

Techniques: Degradation Assay, Immunofluorescence, Microscopy, Clinical Proteomics, Membrane, Immunolabeling, Proximity Ligation Assay, Protein Binding, Western Blot, Control, Knockdown

(A and B) Rescue of Ca V 1.2 degradation in cKO primary beta cells in the presence of CHX by leupeptin (Leu) or MG-132 (MG) for 4 h. ns, p > 0.05; * p < 0.05; one-way ANOVA, n = 12. (C and D) Vesicle IP of MG-132-treated MIN6 cell lysates transduced with scrambled control (SC) and KIF5B-knockdown (KD) miRNAs, precipitated using Ca V 1.2 or K ir 6.2 antibodies or normal rabbit IgG (NRG) and immunoblotted for the indicated proteins (C), accompanied by quantification of Ca V 1.2-coprecipitated fractions (D). Note that the Ca V 1.2-binding capacities of derlin-1, calnexin-1, and Hsp90 chaperones and that of the adaptor protein STIP1 in KD cell lysates were significantly lower than those in SC cell lysates. (E) Vesicle IP of the MG-132-treated MIN6 cell lysates among the KIF5B KD system against STIP1. Note that the Hsp90 level in the STIP1 immunoprecipitants (IP) was greatly decreased by KIF5B deficiency. Repeated twice. (F) Schematic representation of the working hypothesis on differential KIF5B- and heat-shock-protein (Hsp)-dependencies of opposing ER clients Ca V 1.2 and K ir 6.2 in control (CT) and KIF5B conditional knockout (cKO) mouse beta cells. In cKO cells, Ca V 1.2 fails in chaperone exchange to undergo ERAD-mediated degradation, but K ir 6.2 is intact because it is independent on the KIF5B–Hsp machinery. (G and H) Ca V 1.2 immunocytochemistry of MIN6 cells that had been transduced with EYFP-KIF5B and/or TagRFP-Hsc70 or without them (NT; G); accompanied by their quantification (H). Scale bar, 5 μm. ns, p > 0.05; ** p < 0.01, one-way ANOVA, n = 5–13. Arrow in G, enhanced Ca V 1.2 expression according to dual overexpression. (I) Vesicle IP of non-transduced (NT) and KIF5B- and Hsc70-overexpressing (K5+H70 OE) MIN6 cell lysates against Ca V 1.2. Asterisks, tagged protein bands. The tagRFP-Hsc70 band was overlapped with a band of possibly ubiquitinated form. Reproduced twice.

Journal: bioRxiv

Article Title: Kinesin-1 mediates proper ER folding of the Ca V 1.2 channel and maintains mouse glucose homeostasis

doi: 10.1101/2024.06.24.600327

Figure Lengend Snippet: (A and B) Rescue of Ca V 1.2 degradation in cKO primary beta cells in the presence of CHX by leupeptin (Leu) or MG-132 (MG) for 4 h. ns, p > 0.05; * p < 0.05; one-way ANOVA, n = 12. (C and D) Vesicle IP of MG-132-treated MIN6 cell lysates transduced with scrambled control (SC) and KIF5B-knockdown (KD) miRNAs, precipitated using Ca V 1.2 or K ir 6.2 antibodies or normal rabbit IgG (NRG) and immunoblotted for the indicated proteins (C), accompanied by quantification of Ca V 1.2-coprecipitated fractions (D). Note that the Ca V 1.2-binding capacities of derlin-1, calnexin-1, and Hsp90 chaperones and that of the adaptor protein STIP1 in KD cell lysates were significantly lower than those in SC cell lysates. (E) Vesicle IP of the MG-132-treated MIN6 cell lysates among the KIF5B KD system against STIP1. Note that the Hsp90 level in the STIP1 immunoprecipitants (IP) was greatly decreased by KIF5B deficiency. Repeated twice. (F) Schematic representation of the working hypothesis on differential KIF5B- and heat-shock-protein (Hsp)-dependencies of opposing ER clients Ca V 1.2 and K ir 6.2 in control (CT) and KIF5B conditional knockout (cKO) mouse beta cells. In cKO cells, Ca V 1.2 fails in chaperone exchange to undergo ERAD-mediated degradation, but K ir 6.2 is intact because it is independent on the KIF5B–Hsp machinery. (G and H) Ca V 1.2 immunocytochemistry of MIN6 cells that had been transduced with EYFP-KIF5B and/or TagRFP-Hsc70 or without them (NT; G); accompanied by their quantification (H). Scale bar, 5 μm. ns, p > 0.05; ** p < 0.01, one-way ANOVA, n = 5–13. Arrow in G, enhanced Ca V 1.2 expression according to dual overexpression. (I) Vesicle IP of non-transduced (NT) and KIF5B- and Hsc70-overexpressing (K5+H70 OE) MIN6 cell lysates against Ca V 1.2. Asterisks, tagged protein bands. The tagRFP-Hsc70 band was overlapped with a band of possibly ubiquitinated form. Reproduced twice.

Article Snippet: A rabbit anti-Ca V 1.2 antibody (N-17-R, #sc-16229-R, RRID:AB_2228387), a rabbit anti-K ir 6.2 antibody (H-55; #sc-20809; RRID:AB_2130466), a goat anti-PIP5Kα (PIPKIα) antibody (M-20, #sc-11775; RRID:AB_2268303), and a goat anti-SUR1 antibody (N-18, #sc-11226; RRID:AB_2130475) were purchased from Santa Cruz Biotechnology; a mouse anti-PIP 2 IgM antibody (#Z-A045, RRID:AB_427211) was from Echelon Research labs; a rabbit anti-GFP antibody (#598, RRID:AB_591819) was from MBL; a mouse anti-LC3 antibody (Clone LC3-1703, #CTB-LC3-2-IC, RRID:AB_10707197) was from Cosmo Bio; a rabbit anti-BK Ca (K Ca 1.1) antibody (#APC-151, RRID:AB_10915895) and a rabbit anti-Ca V 2.3 antibody (#ACC-006, RRID:AB_2039777) were from Alomone Labs; a mouse anti-syntaxin-1 antibody (#MAB336, RRID:AB_2196527) was from Millipore; a mouse anti-Na/K ATPase beta 2 antibody (#610914; RRID:AB_398231) and a mouse anti-paxillin antibody (#610051, RRID:AB_397463) were from BD Transduction Labs; a mouse anti-Hsc70/Hsp70 antibody (Clone BB70, #ADI-SPA-822-D, RRID:AB_2039252) and a rat anti-Hsp90 antibody (Clone 16F1, #ADI-SPA-835-D, RRID:AB_2039281) were from Enzo; a rabbit anti-STIP1 antibody (#15218-1-AP; RRID:AB_2255518) and a rabbit anti-calnexin-1 (CANX) antibody (#10427-2-AP, RRID:AB_2069033) were from Proteintech; a mouse anti-derlin-1 antibody (#SAB4200148, RRID:AB_10624068), an anti-α-tubulin antibody (Clone DM1A; #CP06; RRID:AB_2617116), and an anti-insulin antibody (Clone K36aC10, #I-2018, Sigma Aldrich; RRID:AB_260137) were from Sigma Aldrich; a rabbit anti-phospho Src antibody for pSFK (#ab32078; RRID:AB_2286707) was from Epitomics/Abcam; and a rabbit anti-KIF5B antibody (RRID:AB_2571745) was previously described ( Tanaka et al ., 1998 ).

Techniques: Transduction, Control, Knockdown, Binding Assay, Knock-Out, Immunocytochemistry, Expressing, Over Expression

Psychotropic drugs induce mitochondrial membrane depolarization. Mitochondrial membrane potential depolarization was evaluated by JC-1 staining after overnight treatment with psychotropic compounds (5 μmol/L) in MCF7 cells. Pictures were acquired by fluorescence microscopy. Representative images of cell treated with the negative control DMSO, carbonyl cyanide 4-[trifluoromethoxy]phenylhydrazone (FCCP), positive control, ebastine, fluoxetine, fluspirilene, nefazodone, penfluridol, pimozide, and spiperone (A) . Histogram showing quantification of red/green fluorescent ratio as fold change relative to control (B) . Data are presented as mean ± SD from three independent experiments, each performed in triplicate. **, Student's T -test p < 0.01; ***, Student's T -test p < 0.001.

Journal: Frontiers in Oncology

Article Title: Psychotropic Drugs Show Anticancer Activity by Disrupting Mitochondrial and Lysosomal Function

doi: 10.3389/fonc.2020.562196

Figure Lengend Snippet: Psychotropic drugs induce mitochondrial membrane depolarization. Mitochondrial membrane potential depolarization was evaluated by JC-1 staining after overnight treatment with psychotropic compounds (5 μmol/L) in MCF7 cells. Pictures were acquired by fluorescence microscopy. Representative images of cell treated with the negative control DMSO, carbonyl cyanide 4-[trifluoromethoxy]phenylhydrazone (FCCP), positive control, ebastine, fluoxetine, fluspirilene, nefazodone, penfluridol, pimozide, and spiperone (A) . Histogram showing quantification of red/green fluorescent ratio as fold change relative to control (B) . Data are presented as mean ± SD from three independent experiments, each performed in triplicate. **, Student's T -test p < 0.01; ***, Student's T -test p < 0.001.

Article Snippet: FCCP (Cayman chemicals) was added for 15 min after the staining as positive control.

Techniques: Membrane, Staining, Fluorescence, Microscopy, Negative Control, Positive Control, Control