Journal: BMC cancer

Article Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells.

doi: 10.1186/s12885-023-11129-1

Figure Lengend Snippet: Fig. 1 TCP11 gene is highly expressed in cervical cancer tissues and cells, and is closely associated with patients’ survival rate. A: The mRNA expression of TCP11 in normal cervical tissues and cervical cancer tissues were analyzed by GEPIA database. B: Immunohistochemical results of TCP11 protein expres sion in normal cervical tissues (n = 31) and cervical cancer tissues (n = 35) microarray. “-” indicates that the expression of TCP11 is negative; “+” indicates that the expression of TCP11 is weakly positive; “++” indicates that the expression of TCP11 is medium positive. Magnification: ×100 (top) and ×400 (bottom). C: Western blot was used to detect the expression of TCP11 protein in immortalized epithelial cells HaCaT and three cervical cancer cells. Full-length blots/ gels are presented in Supplementary Figure S1. D: qRT-PCR was used to detect the expression of TCP11 mRNA in immortalized epithelial cells HaCaT and three cervical cancer cells. E: GEPIA database was used to analyze the relationship between the expression of TCP11 and survival rate of cervical cancer patients. Data are presented as mean ± SD of at least 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: After incubation in the dark in 3% H2O2, they were incubated with TCP11 antibody (Proteintech, China, 1:200) overnight.

Techniques: Expressing, Immunohistochemical staining, Microarray, Western Blot, Quantitative RT-PCR

Journal: BMC cancer

Article Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells.

doi: 10.1186/s12885-023-11129-1

Figure Lengend Snippet: Fig. 2 TCP11 overexpression inhibits the proliferation of HeLa and SiHa cells. A: Western blot was used to detect the overexpression of TCP11 protein in HeLa and SiHa cells after lentivirus infection. Full-length blots/gels are presented in Supplementary Figure S2. B: qRT-PCR was used to detect the overex pression of TCP11 mRNA in HeLa and SiHa cells after lentivirus infection. C and D: The cell viability of HeLa and SiHa cells was detected by MTT and colony formation assays. E: The protein expression of Ki67 in HeLa and SiHa cells was detected by cellular immunohistochemistry. Magnification: ×100. The results were analyzed by Image Pro Plus software, and the mean density was calculated (mean density = IOD/area). F: Ki67 mRNA expression in HeLa and SiHa cells were detected by qRT-PCR. Data are presented as mean ± SD of at least 3 independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001

Article Snippet: After incubation in the dark in 3% H2O2, they were incubated with TCP11 antibody (Proteintech, China, 1:200) overnight.

Techniques: Over Expression, Western Blot, Infection, Quantitative RT-PCR, Expressing, Immunohistochemistry, Software

Journal: BMC cancer

Article Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells.

doi: 10.1186/s12885-023-11129-1

Figure Lengend Snippet: Fig. 3 TCP11 overexpression blocks cell cycle progression in HeLa and SiHa cells. A and B: Cell cycle distribution of HeLa and SiHa cells was determined by flow cytometry. C: Western blot was used to detect the protein expression of CDK1 and Cyclin B1 in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Full-length blots/gels are presented in Supplementary Figure S3. D: qRT-PCR was used to detect the mRNA expression of CDK1 and Cyclin B1 in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Data are presented as mean ± SD of at least 3 independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001

Article Snippet: After incubation in the dark in 3% H2O2, they were incubated with TCP11 antibody (Proteintech, China, 1:200) overnight.

Techniques: Over Expression, Flow Cytometry, Western Blot, Expressing, Infection, Quantitative RT-PCR

Journal: BMC cancer

Article Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells.

doi: 10.1186/s12885-023-11129-1

Figure Lengend Snippet: Fig. 4 TCP11 overexpression induces the apoptosis of HeLa and SiHa cells. A and B: Apoptosis of HeLa and SiHa cells were detected by flow cytometry. C: Western blot was used to detect the protein expression of caspase-3, cleaved-caspase-3 and cleaved-PARP in HeLa and SiHa cells infected with over expressing TCP11 lentivirus. Full-length blots/gels are presented in Supplementary Figure S4. Data are presented as mean ± SD of at least 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: After incubation in the dark in 3% H2O2, they were incubated with TCP11 antibody (Proteintech, China, 1:200) overnight.

Techniques: Over Expression, Flow Cytometry, Western Blot, Expressing, Infection

Journal: BMC cancer

Article Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells.

doi: 10.1186/s12885-023-11129-1

Figure Lengend Snippet: Fig. 5 TCP11 overexpression inhibits cervical cancer cell migration by inhibiting EMT of HeLa and SiHa cells. A and B: Cell scratch assay was used to detect the migration ability of HeLa and SiHa cells. C and D: Transwell migration assay was used to detect the migration ability of HeLa and SiHa cells. E: Western blot was used to detect the protein expression of EMT-related molecules ZO-1, E-cadherin, Claudin-1, Snail, Vimentin and β-catenin in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Full-length blots/gels are presented in Supplementary Figure S5. F: qRT-PCR was used to detect the mRNA expression of EMT-related molecules ZO-1 and E-cadherin in HeLa and SiHa cells infected with overexpressing TCP11 lentivirus. Data are presented as mean ± SD of at least 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: After incubation in the dark in 3% H2O2, they were incubated with TCP11 antibody (Proteintech, China, 1:200) overnight.

Techniques: Over Expression, Migration, Wound Healing Assay, Transwell Migration Assay, Western Blot, Expressing, Infection, Quantitative RT-PCR

Journal: BMC cancer

Article Title: Increased TCP11 gene expression can inhibit the proliferation, migration and promote apoptosis of cervical cancer cells.

doi: 10.1186/s12885-023-11129-1

Figure Lengend Snippet: Fig. 6 TCP11 knockdown promotes the proliferation of HeLa and SiHa cells and the migration of HeLa cells. A: Western blot was used to detect the knock down effect of three designed and constructed siRNA fragments on TCP11 in HeLa and SiHa cells. Full-length blots/gels are presented in Supplementary Figure S6. B: qRT-PCR was used to detect the knockdown effect of three siRNA fragments on TCP11 in HeLa and SiHa cells. C: MTT assay was used to detect the effect of TCP11 knockdown on the proliferation of HeLa and SiHa cells. D: Colony formation assay was used to detect the effect of TCP11 knockdown on the proliferation of HeLa cells. E: Transwell migration assay was used to detect the effect of TCP11 knockdown on the migration of HeLa cells. Data are presented as mean ± SD of at least 3 independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001

Article Snippet: After incubation in the dark in 3% H2O2, they were incubated with TCP11 antibody (Proteintech, China, 1:200) overnight.

Techniques: Knockdown, Migration, Western Blot, Construct, Quantitative RT-PCR, MTT Assay, Colony Assay, Transwell Migration Assay

Journal: Stem Cell Research & Therapy

Article Title: Restoration of NOX4 signalling reverses endothelial colony-forming cell angiogenic dysfunction associated with experimental and clinical diabetes

doi: 10.1186/s13287-025-04393-4

Figure Lengend Snippet: Clinical diabetes induces dysregulation of CB-ECFC angiogenic pathways linked with downstream NOX4-dependent pro-angiogenic signalling. CB-ECFCs were isolated from healthy and gestational diabetic donors and subjected to RNA sequencing prior to ( A ) IPA network generation, node colour represents predicted gene activation (orange) and inhibition (blue). B , C Proteome Profiler™ analysis of pooled lysates (triplicates from 5 clones) from diabetic CB-ECFCs subjected to electroporation for introduction of either empty vector (EV; pcDNA4/TO/myc-His A) or NOX4 overexpression (OE; pcDNA4/TO/NOX4-myc-His A) construct for detection of protein phosphorylation. Differential expression between groups presented as a heat map indicating largely decreased (red) phosphorylation (OE versus EV). D – G Protein expression of endoglin, E2F1 and SERPINE1 by Western blotting with normalisation to ACTB as loading control; n = 8/9, combined data from 3 different clones. Representative cropped blots shown from one healthy and one diabetic clone. Full-length blots/gels are presented in Supplementary Figure. Data are mean ± SEM; ** P < 0.01, *** P < 0.001, paired Student’s t -test

Article Snippet: Membranes were then incubated overnight at 4 °C with primary antibodies against ACTB (#3700, Cell Signaling Technology, 1:5000), NOX2 (ab80508, Abcam, 1:2000), NOX4 (ab133303, Abcam, 1:2000), VEGF Receptor 2 (55B11, Cell Signaling Technology, 1:1000), Phospho-VEGF Receptor 2 (Try 1175; D5B11, Cell Signaling Technology, 1:1000), eNOS (610297, BD Transduction Labs, 1:500), Phospho-eNOS (S1177, 9571S Cell Signaling Technology, 1:1000), SERPINE1 (66261-1-Ig, Proteintech, 1:10000), Endoglin (#14606, Cell Signaling Technology, 1:1000) and E2F1 (66515-1-Ig, Proteintech, 1:3000).

Techniques: Isolation, RNA Sequencing, Activation Assay, Inhibition, Clone Assay, Electroporation, Plasmid Preparation, Over Expression, Construct, Phospho-proteomics, Quantitative Proteomics, Expressing, Western Blot, Control

Journal: Stem Cell Research & Therapy

Article Title: Restoration of NOX4 signalling reverses endothelial colony-forming cell angiogenic dysfunction associated with experimental and clinical diabetes

doi: 10.1186/s13287-025-04393-4

Figure Lengend Snippet: Restoration of NOX4 expression in diabetic CB-ECFCs rescues angiogenic capacity by specifically inducing pro-angiogenic signalling. Summary schematic indicating that restoration of NOX4 levels in diabetic CB-ECFCs leads to upregulated E2F1 signalling in parallel with fully restored angiogenic function. E2F1 is a transcription factor which positively regulates key genes associated with efficient progression through the cell cycle, DNA replication and subsequent proliferation, which is critical to support an efficient and potent pro-angiogenic response. NOX4 induction in diabetic CB-ECFCs also led to reduced phosphorylation of anti-proliferative P53 (at S46), increased expression of SERPINE1 and endoglin, which are implicated in pro-angiogenic signalling

Article Snippet: Membranes were then incubated overnight at 4 °C with primary antibodies against ACTB (#3700, Cell Signaling Technology, 1:5000), NOX2 (ab80508, Abcam, 1:2000), NOX4 (ab133303, Abcam, 1:2000), VEGF Receptor 2 (55B11, Cell Signaling Technology, 1:1000), Phospho-VEGF Receptor 2 (Try 1175; D5B11, Cell Signaling Technology, 1:1000), eNOS (610297, BD Transduction Labs, 1:500), Phospho-eNOS (S1177, 9571S Cell Signaling Technology, 1:1000), SERPINE1 (66261-1-Ig, Proteintech, 1:10000), Endoglin (#14606, Cell Signaling Technology, 1:1000) and E2F1 (66515-1-Ig, Proteintech, 1:3000).

Techniques: Expressing, Phospho-proteomics

Journal: BMC Microbiology

Article Title: Niche differentiation in nitrogen metabolism among methanotrophs within an operational taxonomic unit

doi: 10.1186/1471-2180-14-83

Figure Lengend Snippet: Nitrogen assimilation and tolerance per strain

Article Snippet: In addition, R-49807 unexpectedly did not exhibit N fixation, as sole M. koyamae strain, despite a >99% nifH gene sequence similarity with strains NCIMB 14606 T and R-49799, both positive for N fixation under low oxygen tension.

Techniques: