Journal: Frontiers in Physiology

Article Title: Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues

doi: 10.3389/fphys.2021.817181

Figure Lengend Snippet: List of antibodies used on human kidney tissue.

Article Snippet: CD14 , Monocytes/Macrophages , Rabbit , EPR3653 , 144Nd , 1:50 , Fluidigm , .

Techniques: Membrane

Journal: Scientific Reports

Article Title: HLA-DR cancer cells expression correlates with T cell infiltration and is enriched in lung adenocarcinoma with indolent behavior

doi: 10.1038/s41598-021-93807-3

Figure Lengend Snippet: Mass cytometry antibody panel for lung adenocarcinoma.

Article Snippet: HLA-ABC , 144-Nd , Surface , W6/32 , Fluidigm , 3144017B.

Techniques: Mass Cytometry

Journal: iScience

Article Title: Single-cell transcriptome and crosstalk analysis reveals immune alterations and key pathways in the bone marrow of knee OA patients

doi: 10.1016/j.isci.2024.110827

Figure Lengend Snippet:

Article Snippet: Anti-Human CD31 (WM59)-144ND , Fluidigm Corporation , Cat#3144023B.

Techniques: Recombinant, Software

Journal: Molecular Oncology

Article Title: Single‐cell protein profiling defines cell populations associated with triple‐negative breast cancer aggressiveness

doi: 10.1002/1878-0261.13365

Figure Lengend Snippet: Overview of antibodies and reagents used in study.

Article Snippet: CD69 , FN50 , 144Nd , Fluidigm , 3144018B.

Techniques: Cytometry, Marker

Journal: Science translational medicine

Article Title: Mixed-Effects Association of Single Cells Identifies an Expanded Effector CD4+ T Cell Subset in Rheumatoid Arthritis

doi: 10.1126/scitranslmed.aaq0305

Figure Lengend Snippet: RNA-seq characterization of CD627- HLA-DR+ (DR+27-) cells and 6 related CD4+ T cell populations: naive T cells (Tnaive), regulatory T cells (Treg), central memory t cells (TCM), and three populations of effector memory T cells, CD27+ HLA-DR- (DR-27+), CD27+ HLA-DR+ (DR+27+), and CD27- HLA-DR- (DR-27-). (A) PCA plot (top) and PC1 gene loadings (bottom) of 90 samples from the 7 CD4+ T cell populations. Cells were colored on the PCA plot according to known cell type. Normal confidence ellipses at 1 standard deviation were plotted for each cell type. The 300 most positive and 300 most negative PC1 gene loadings for each cell type were averaged and plotted in the heatmap. Genes relevant to the CD27- HLA-DR+ population were labeled. (B) Gene set enrichment analysis was performed on all genes, ranked on their PC1 loadings. Two significantly enriched gene sets: NK signature (GSE22886 NAIVE CD4 T CELL VS NK CELL DN) and effector memory t cell signature (GSE11057 NAIVE VS EFF MEMORY CD4 T CELL) are shown. (C) Distribution of log-scaled expression of six canonical Th1 genes: CCR5, CIITA, CXCR3, IFNG, TBX21 (Tbet), and TNF. Populations are ordered by PC1 loading values, with CD27- HLA-DR+ population highlighted in red. (D) Distribution of log-scaled gene expression of six canonical cytotoxic genes: GNLY, GZMA, GZMB, GMZK, NKG7, and PRF1. Populations are ordered by PC1 loading values, with the CD27- HLA-DR+ population highlighted in red. Reported p-values in (C) and (D) correspond to a linear model of gene expression against ordered cell type (as an ordinal variable), with p-values adjusted for multiple testing by the Benjamini Hochberg procedure. (E) Cytokine expression determined by intracellular cytokine staining of peripheral effector memory CD4+ T cells after in vitro stimulation with PMA/ionomycin. The percentage of cells positive for each stain is plotted for CD27+ HLA-DR- and CD27- HLA-DR+ subsets. Each dot represents a separate donor (n = 12; 6 RA patients and 6 controls, except for the quantification of Granyzme A and perforin where n = 6; 3 RA patients and 3 controls). Statistical significance was assessed using a Wilcoxon signed-rank test.

Article Snippet: The samples were then incubated for 30 minutes at 4C on a shaker rack with 1ul of the following eighteen CyTOF surface antibodies in a cocktail brought to a volume of 50ul/sample in CSB: Anti-Human CD49D (9F10)-141Pr (Fluidigm), Anti-Human CCR5 (CD195)(–P-6G4) - 144Nd (Fluidigm), Anti-Human CD4 (RPA-T4) −145Nd (Fluidigm), Anti-Human CD8a (RPA-T8) −146Nd (Fluidigm), Anti-Human CD45RO-147Sm (Brigham and Women’s Hospital CyTOF Core), Anti-Human CD28–148Nd (BWH CyTOF Core), Anti-Human CD25 (IL-2R- (2A3) - 149Sm (Fluidigm), Anti-Human PD1–151Eu (BWH CyTOF Core), Anti-Human CD62L (DREG-56)-153Eu (Fluidigm), Anti-Human CD3 (UCHT1) −154Sm (Fluidigm), Anti-Human CD27 (L128) −155Gd (Fluidigm), Anti-Human CD183[CXCR3](G025H7)-156Gd (Fluidigm), Anti-Human CCR7–170Er (BWH CyTOF Core), Anti-Human ICOS-160Gd (BWH CyTOF Core), Anti-Human CD38 (HIT2)-167Er (Fluidigm), Anti-Human CD154 (CD40L) (24–31)-168Er (Fluidigm), Anti-Human CXCR5[CD185](51505)-171Yb (Fluidigm), and Anti-Human HLA-DR (L243) −174Yb (Fluidigm).

Techniques: RNA Sequencing, Standard Deviation, Labeling, Expressing, Gene Expression, Staining, In Vitro

Journal: STAR Protocols

Article Title: MATISSE: An analysis protocol for combining imaging mass cytometry with fluorescence microscopy to generate single-cell data

doi: 10.1016/j.xpro.2021.101034

Figure Lengend Snippet:

Article Snippet: Maxpar X8 144Nd Labeling Kit – 4 Rxn , Fluidigm , Cat# 201144A.

Techniques: Recombinant, Blocking Assay, Labeling, Immunostaining, Software, Microscopy, Modification, Adhesive, Staining, Imaging