Journal: Journal of Nanobiotechnology

Article Title: Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain

doi: 10.1186/s12951-016-0158-0

Figure Lengend Snippet: The degradation of different chlorophenols by free resting cells of Rhodococcus rhodochrous DSM6263. The substrates used were a 2-chlorophenol, b 4-chlorophenol, c 2,3-dichlorophenol and d 2,4,6-trichlorophenol, respectively. The initial chlorophenol concentrations were 0.5 mM. The medium without strain inoculation was used as blank control to exclude any possible photodegradation

Article Snippet: Rhodococcus rhodochrous DSM6263 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ).

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain

doi: 10.1186/s12951-016-0158-0

Figure Lengend Snippet: Biodegradation of chlorophenols by immobilized cells of Rhodococcus rhodochrous DSM6263 with different immobilization supports. a 2-chlorophenol, b 4-chlorophenol and c 2,3-dichlorophenol. The initial chlorophenol concentrations were 0.25 mM. The free active cells and inactive cells (autoclaved at 115 °C for 5 min) were used as positive and negative controls, respectively. The error bars in the figure indicate the standard deviation of three parallel replicates

Article Snippet: Rhodococcus rhodochrous DSM6263 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ).

Techniques: Standard Deviation

Journal: Journal of Nanobiotechnology

Article Title: Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain

doi: 10.1186/s12951-016-0158-0

Figure Lengend Snippet: Biodegradation of phenol and chlorophenol mixture by magnetic immobilized cells of Rhodococcus rhodochrous DSM6263. k -Carrageenan and 9 g/L Fe 3 O 4 NPs were used as the immobilization support. The mixture contained phenol, 2-chlorophenol, 4-chlorophenol and 2,3-dichlorophenol. Each compound was added at the initial concentration of 0.25 mM, respectively. The error bars in the figure indicate the standard deviation of three parallel replicates

Article Snippet: Rhodococcus rhodochrous DSM6263 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ).

Techniques: Concentration Assay, Standard Deviation

Journal: Journal of Nanobiotechnology

Article Title: Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain

doi: 10.1186/s12951-016-0158-0

Figure Lengend Snippet: Repeated use of magnetically immobilized cells of Rhodococcus rhodochrous DSM6263 for 2-chlorophenol degradation. k -Carrageenan and 9 g/L Fe 3 O 4 NPs were used as the immobilization support. T1 to T7 mean the recycle times. The error bars in the figure indicate the standard deviation of three parallel replicates

Article Snippet: Rhodococcus rhodochrous DSM6263 was purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ).

Techniques: Standard Deviation

Journal: Cell Discovery

Article Title: A positive mechanobiological feedback loop controls bistable switching of cardiac fibroblast phenotype

doi: 10.1038/s41421-022-00427-w

Figure Lengend Snippet: a Ca 2+ (Fluo-4 AM) imaging of CFs transfected with siRNA targeting Piezo1 (siPiezo1) or with negative control siRNA (siNT), showing reduction of mechanosensitive cytosolic Ca 2+ elevation after 3 days of culture in CFs with Piezo1 knocked down. Scale bar, 50 μm. b Quantification of fluorescence intensity data from panel a ( n ≥ 33 cells). c Immunofluorescence analysis indicated that nuclear YAP decreased following siPiezo1 inhibition of Piezo1 (blue, nucleus (DAPI); green, YAP; red, F-actin). Scale bar, 10 μm. d Quantification of the YAP nuclear-to-cytoplasmic ratio from images represented by those in panel c ( n ≥ 12 cells). e RT-PCR analysis of downstream YAP genetic targets ( CYR61 and CTGF ) in CFs with or without Piezo1 knockdown by siPiezo1. f Immunofluorescence analysis revealed that α-SMA (green), a marker of CF differentiation, was attenuated by the Piezo1 blocker GsMTx4, but that mechanosensitive α-SMA expression could be rescued by transfecting CFs to produce excess YAP. Scale bar, 50 μm. g RT-PCR analysis of α-SMA, Col 1, PDGFR-α and TGF-β1 in YAP-overexpressing CFs in the presence of GsMTx4 indicates that CF activation in response to matrix stiffness is enhanced by Piezo1 and can be rescued by YAP overexpression. h Schematic diagram of signaling downstream of the PFL.

Article Snippet: The primary antibodies used in our study include CD29 (Integrin β1) monoclonal antibody (1:200; Thermo Fisher Scientific, 13-0291-80, USA), α-SMA-FITC antibody (1:400; Sigma, F3777, USA), Piezo1 antibody (1: 100; Affinity, DF12083, China), YAP antibody (Alexa Fluor ® 488 Conjugate; 1:100; Cell Signaling Technology, 14729, USA), vinculin antibody (1:250; Abcam, EPR8185, England), paxillin antibody (1:250; Abcam, Y113, England), and Ki-67 (D3B5) antibody (1:400; Cell Signaling Technology, 9129, USA) at 4 °C overnight.

Techniques: Imaging, Transfection, Negative Control, Fluorescence, Immunofluorescence, Inhibition, Reverse Transcription Polymerase Chain Reaction, Marker, Expressing, Activation Assay, Over Expression