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The targeting vector has been generated using C57BL/6J DNA and has been transfected into TaconicArtemis C57BL/6N Tac ES cell line.
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Image Search Results
Journal: Nature microbiology
Article Title: SARS-CoV-2 Delta and Omicron variants evade population antibody response by mutations in a single spike epitope.
doi: 10.1038/s41564-022-01235-4
Figure Lengend Snippet: Fig. 4 | R1-32 binding resulted in destruction of SARS-CoV-2 spike structure. a,b, Ligand-induced conformational change assays to probe the induction of post-fusion structures. a, Native spike (S-R) (7.09 µM) was incubated with molar excess of ACE2-Fc or antibodies for 1 h. Samples were analysed by western blotting to detect generation of the 55 kDa proteinase K-resistant core, which is a signature of the post-fusion S2 structure62. ACE2-Fc and class 1 antibodies B38 and rmAb23 induced post-fusion structures. b, Inhibition of conformational change by non-ACE2 competing antibodies were assayed by antibody pre- incubation (1 h) before further incubation (1 h) with ACE2-Fc. Only R1-32 was able to abolish fusogenic spike conformational change among the tested non-ACE2 competing antibodies. Representative data are shown from at least
Article Snippet: Wild-type authentic SARS-CoV-2 viruses: SARS-CoV-2/human/ CHN/IQTC01/2020 used for animal experiment was isolated from COVID-19 patients in Guangzhou (GenBank accession no. MT123290)39; 2019-nCoV BetaCoV/Wuhan/WIV04/2019 (GenBank accession no. MN996528) is stored at Microorganisms and Viruses Culture Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences (CSTR: 1633.06.IVCAS 6.7512)40; SARS-CoV-2 Beta variant (CSTR: 1633.06.IVCAS.6.7552) and Delta variant (CSTR.16698.06.NPRC 6.CCPM-B-V-049-2105-8) are stored at the National Pathogen Resource Center (NPRC). hACE2 transgenic mice The
Techniques: Binding Assay, Incubation, Western Blot, Inhibition