Journal: mAbs

Article Title: Fc-mediated activity of EGFR x c-Met bispecific antibody JNJ-61186372 enhanced killing of lung cancer cells

doi: 10.1080/19420862.2016.1249079

Figure Lengend Snippet: Increased FcγRIIIa binding of low-and hemi-fucosylated forms of JNJ-61186372. FcγRI (A), FcγRIIa (B), and FcγRIIIa (C) binding by antibodies produced in normal fucose (NF) or low fucose (LF) cell line was assessed by competitive Alpha Screen and compared to a wild type IgG1 control antibody (closed circle). JNJ-61186372 – NF (closed square), JNJ-61186372 – LF (closed up triangle), EGFR x inert arm –LF (closed down triangle), c-Met x inert arm – LF (open up triangle), EGFR (LF) x c-Met (NF) (open down triangle), EGFR (NF) x c-Met (LF) (open diamond), JNJ-61186372 – IgG2σ (open circle). Representative data from 3 replicate experiments is shown.

Article Snippet: His-tagged FcγRI (R&D Systems, cat no. 1257-FC), FcγRIIa (R&D Systems, cat no. 1330-CD/CF) or FcγRIIIa (R&D Systems, cat no. 4325-FC) were captured on nickel chelate acceptor beads (PerkinElmer, cat. no. CUSM0220400EA).

Techniques: Binding Assay, Produced, Control

Journal: mAbs

Article Title: Fc-mediated activity of EGFR x c-Met bispecific antibody JNJ-61186372 enhanced killing of lung cancer cells

doi: 10.1080/19420862.2016.1249079

Figure Lengend Snippet: Concentration of antibody (nM) to elicit 50% of maximal FcγR binding activity (IC 50 ). Data presented in mean ± SEM of 2–3 independent experiments. *Difference between JNJ-61186372 - LF and JNJ-61186372 – NF is statistically significant (p value = 0.0001).

Article Snippet: His-tagged FcγRI (R&D Systems, cat no. 1257-FC), FcγRIIa (R&D Systems, cat no. 1330-CD/CF) or FcγRIIIa (R&D Systems, cat no. 4325-FC) were captured on nickel chelate acceptor beads (PerkinElmer, cat. no. CUSM0220400EA).

Techniques: Concentration Assay, Binding Assay, Activity Assay, Significance Assay

Journal: mAbs

Article Title: Fc-mediated activity of EGFR x c-Met bispecific antibody JNJ-61186372 enhanced killing of lung cancer cells

doi: 10.1080/19420862.2016.1249079

Figure Lengend Snippet: Fold change to multiple FcγR binding (IC 50 ) of different antibodies to that of JNJ-61186372 – NF.

Article Snippet: His-tagged FcγRI (R&D Systems, cat no. 1257-FC), FcγRIIa (R&D Systems, cat no. 1330-CD/CF) or FcγRIIIa (R&D Systems, cat no. 4325-FC) were captured on nickel chelate acceptor beads (PerkinElmer, cat. no. CUSM0220400EA).

Techniques: Binding Assay

Journal: Current Protocols

Article Title: Cryopreservation of Human Adult Ventricular Tissue for the Preparation of Viable Myocardial Slices

doi: 10.1002/cpz1.70068

Figure Lengend Snippet: Primary Antibodies

Article Snippet: Type III Collagen , 1:2000 , Southern Biotech , 1330‐01.

Techniques:

Journal: Current Protocols

Article Title: Cryopreservation of Human Adult Ventricular Tissue for the Preparation of Viable Myocardial Slices

doi: 10.1002/cpz1.70068

Figure Lengend Snippet: Extracellular matrix. Representative images of immunohistochemical analysis of fresh and cryopreserved cardiac tissue. Type I Collagen (Collagen‐I) is shown in orange (A) and Type III Collagen (Collagen‐III) is shown in yellow (B) . Counterstaining of nuclei was performed with DAPI (blue).

Article Snippet: Type III Collagen , 1:2000 , Southern Biotech , 1330‐01.

Techniques: Immunohistochemical staining