12ca5 Search Results


10 nm diameter gold anti ha tag  (Bioss)
90
Bioss 10 nm diameter gold anti ha tag
10 Nm Diameter Gold Anti Ha Tag, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10 nm diameter gold anti ha tag/product/Bioss
Average 90 stars, based on 1 article reviews
10 nm diameter gold anti ha tag - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
12ca5  (Novus Biologicals)
93
Novus Biologicals 12ca5
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
12ca5, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/12ca5/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
12ca5 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
mouse anti ha  (Bio X Cell)
93
Bio X Cell mouse anti ha
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Mouse Anti Ha, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti ha/product/Bio X Cell
Average 93 stars, based on 1 article reviews
mouse anti ha - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
anti-ha 12ca5 antibody  (Boehringer Mannheim)
90
Boehringer Mannheim anti-ha 12ca5 antibody
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Anti Ha 12ca5 Antibody, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ha 12ca5 antibody/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
anti-ha 12ca5 antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
anti-ha 12ca5 ascites fluid  (Babco Inc)
90
Babco Inc anti-ha 12ca5 ascites fluid
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Anti Ha 12ca5 Ascites Fluid, supplied by Babco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ha 12ca5 ascites fluid/product/Babco Inc
Average 90 stars, based on 1 article reviews
anti-ha 12ca5 ascites fluid - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
monoclonal anti-ha 12ca5  (Becton Dickinson)
90
Becton Dickinson monoclonal anti-ha 12ca5
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Monoclonal Anti Ha 12ca5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal anti-ha 12ca5/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
monoclonal anti-ha 12ca5 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
antihemagglutinin monoclonal antibody 12 ca5  (Babco Inc)
90
Babco Inc antihemagglutinin monoclonal antibody 12 ca5
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Antihemagglutinin Monoclonal Antibody 12 Ca5, supplied by Babco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antihemagglutinin monoclonal antibody 12 ca5/product/Babco Inc
Average 90 stars, based on 1 article reviews
antihemagglutinin monoclonal antibody 12 ca5 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
monoclonal anti-ha (12ca5)-dylight800 control antibody  (Rockland Immunochemicals)
90
Rockland Immunochemicals monoclonal anti-ha (12ca5)-dylight800 control antibody
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Monoclonal Anti Ha (12ca5) Dylight800 Control Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal anti-ha (12ca5)-dylight800 control antibody/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
monoclonal anti-ha (12ca5)-dylight800 control antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
anti-ha-tag (12ca5)  (Covance)
90
Covance anti-ha-tag (12ca5)
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Anti Ha Tag (12ca5), supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ha-tag (12ca5)/product/Covance
Average 90 stars, based on 1 article reviews
anti-ha-tag (12ca5) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
anti-hemagglutinin (ha epitope) antibody (clone 12 ca5)  (Boehringer Mannheim)
90
Boehringer Mannheim anti-hemagglutinin (ha epitope) antibody (clone 12 ca5)
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Anti Hemagglutinin (Ha Epitope) Antibody (Clone 12 Ca5), supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hemagglutinin (ha epitope) antibody (clone 12 ca5)/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
anti-hemagglutinin (ha epitope) antibody (clone 12 ca5) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
psrα5-12ca5 vector  (Affymax Inc)
90
Affymax Inc psrα5-12ca5 vector
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
Psrα5 12ca5 Vector, supplied by Affymax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psrα5-12ca5 vector/product/Affymax Inc
Average 90 stars, based on 1 article reviews
psrα5-12ca5 vector - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
12ca5 monoclonal anti-ha antibody  (Sugen Inc)
90
Sugen Inc 12ca5 monoclonal anti-ha antibody
Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA <t>(12CA5),</t> anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.
12ca5 Monoclonal Anti Ha Antibody, supplied by Sugen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/12ca5 monoclonal anti-ha antibody/product/Sugen Inc
Average 90 stars, based on 1 article reviews
12ca5 monoclonal anti-ha antibody - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA (12CA5), anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.

Journal: G3: Genes | Genomes | Genetics

Article Title: Characterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex

doi: 10.1093/g3journal/jkae221

Figure Lengend Snippet: Comparing the activity of biotin ligase enzymes. Strains containing Not4 tagged with the various biotin ligases indicated in the panel and a background control strain BY4741 were grown in synthetic complete media and then treated (+) with or without (−) 10 μM biotin for 2 hr prior to harvest. a). Western blotting of proteins using anti-HA (12CA5), anti- Dhh1 (loading control) antibodies and streptavidin-HRP (SA-HRP) to detect biotinylated proteins. Two exposures of the SA-HRP blot are presented. The signals of each biotin ligase (+biotin) were quantified in the region of the blot between the Acc1/Hfa1 and Pyc1/2 and between Pyc1/2 and Arc1 bands and were quantified by scanning films and summing the signal and expressed compared to the signal in the BY4742 background strain, which was set to 1.0. The values were: TID, 108.2; mTID 41.6; BASU, 5.9; BioID2, 6.6; BirA* 1.9. b). Immunoprecipitation of extracts of extracts with anti- Not4 antibodies. Immunoprecipitants were analyzed by SA-HRP blots and western blotting for Not4 . HC, heavy chain. The numbers to the left of each panel marks the migration of molecular weight markers in kDa.

Article Snippet: The HA tag was detected using 12CA5 (Novus Biologicals) or HA11.1 (Covance, now Labcorp) monoclonal antibodies.

Techniques: Activity Assay, Control, Western Blot, Immunoprecipitation, Migration, Molecular Weight