Journal: Arthritis and rheumatism

Article Title: In situ analysis of interleukin-23- and interleukin-12-positive cells in the spine of patients with ankylosing spondylitis.

doi: 10.1002/art.37937

Figure Lengend Snippet: Figure 2. A, Immunohistochemical analysis of interleukin-23 (IL-23)– positive cells (red staining) in bone marrow without fibrous tissue revealed a significantly higher frequency of IL-23–positive cells in the facet joints of ankylosing spondylitis (AS) patients compared to osteoarthritis (OA) patients. B, IL-23–positive cells were also identi- fied in fibrous tissue that had replaced bone marrow, with a signifi- cantly higher frequency in the facet joints of OA patients compared to AS patients. C, Immunohistochemical analysis of IL-12–positive cells (brown staining) in bone marrow without fibrous tissue revealed a significantly higher frequency of IL-12–positive cells in the facet joints of AS patients compared to OA patients. D, IL-12–positive cells were also identified in fibrous tissue that had replaced bone marrow, but the frequency of such cells at this site was low both in OA patients and in AS patients, with no significant difference (NS). Graphs show the mean SD of all samples analyzed. Photomicrographs show repre- sentative results. Original magnification 400.

Article Snippet: Immunohistochemistry analysis of paraffin-embedded facet joints was performed to detect IL-23– and IL-12–expressing cells, using an anti-p19 (IL-23) mAb (CNTO 2144; Janssen Research & Development) and an anti-p70 (IL-12) mAb (clone 24910; R&D Systems); both antibodies were used at a dilution of 1:50.

Techniques: Immunohistochemical staining, Staining

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood–brain barrier integrity

doi: 10.1073/pnas.2204700120

Figure Lengend Snippet: Raw average gene expression values from all capillary-derived endothelial cells

Article Snippet: Rabbit anti-EphA4 (Proteintech), goat anti-Cd31 (BD Biosciences), rabbit anti-CD45 (Cell Signaling Technology), rat anti-CD68 (Invitrogen), rabbit anti-Akap12 (Alomone, Jerusalem), and goat anti-Tie2 (R&D Systems).

Techniques: Expressing, Activation Assay, Shear, Membrane, Control

Journal: Micromachines

Article Title: A Novel 3D-Printed and Miniaturized Periodic Counter Current Chromatography System for Continuous Purification of Monoclonal Antibodies

doi: 10.3390/mi15030382

Figure Lengend Snippet: Chromatogram of a continuous membrane adsorber-based CEX run using three membrane adsorbers numbered MA1–MA3. A total of 4 cycles (55 min) and a cleansing post cycle using membrane adsorbers with a volume of 0.41 mL were performed. Small regular plateaus with peaks at the end emerge during loading.

Article Snippet: The consecutive polishing step was performed via cation exchange chromatography using membrane adsorbers (Sartobind ® S15, Sartorius Stedim Biotech, Göttingen, Germany).

Techniques: Membrane