Journal: Cells

Article Title: Alpinetin Suppresses Effects of TGF-β1 on Stimulating the Production and Organization of Fibrotic Markers in Human Primary Dermal Fibroblasts.

doi: 10.3390/cells11172731

Figure Lengend Snippet: Figure 4. AP reverses the action of TGF-β1 on the formation and organization of actin stress fiber in HPDFs at high cell density. Detection of filamentous actin (F-actin) (red) by using DyLight TM 594-Phalloidin in HPDFs (untreated, TGF-β1-treated, or TGF-β1-treated with the presence of 50 µM of AP) at 48 h. Nuclei of cells (blue) were counterstained with DAPI. Figure (a–c) present the untreated cells showing F-actin staining, DAPI staining, and merged images, respectively. Figure (d–f) present TGF-β1-treated cells showing F-actin staining, DAPI staining, and merged images, respectively. Figure (g–i) present cells treated with TGF-β1 and 50 µM of AP showing F-actin staining, DAPI staining, and merged images, respectively. Observation was conducted at 40× magnification. Scale bar = 500 µm.

Article Snippet: In some experiments, filamentous actin (F-actin) was stained with DyLight-594Phalloidin (catalog number12877) (Cell Signaling Technology, Boston, MA, USA).

Techniques: Staining

Journal: Cells

Article Title: Alpinetin Suppresses Effects of TGF-β1 on Stimulating the Production and Organization of Fibrotic Markers in Human Primary Dermal Fibroblasts.

doi: 10.3390/cells11172731

Figure Lengend Snippet: Figure 5. AP blocks the TGF-β1-induced organization of stress fiber and microtubule in individual HPDFs at low cell density. Detection of filamentous actin (F-actin) (red) by using DyLight TM 594-Phalloidin and microtubule (green) by using anti-tubulin antibody in HPDFs (untreated, TGF- β1-treated, or TGF-β1-treated with the presence of 50 µM of AP) at 48 h. Figure (a–c) present the untreated cells showing F-actin staining, tubulin staining, and merged images, respectively. Picture (d–f) present TGF-β1-treated cells showing F-actin staining, tubulin staining, and merged images, respectively. Figure (g–i) present cells treated with TGF-β1 and 50 µM of AP showing F-actin staining, tubulin staining, and merged images, respectively. Observation was conducted at 40× magnification. Scale bar = 500 µm.

Article Snippet: In some experiments, filamentous actin (F-actin) was stained with DyLight-594Phalloidin (catalog number12877) (Cell Signaling Technology, Boston, MA, USA).

Techniques: Staining

Journal: Cells

Article Title: Alpinetin Suppresses Effects of TGF-β1 on Stimulating the Production and Organization of Fibrotic Markers in Human Primary Dermal Fibroblasts.

doi: 10.3390/cells11172731

Figure Lengend Snippet: Figure 9. AP reduces the TGF-β1-stimulated expression of α-SMA and component of type I col- lagen (Col1A1). (A) Immunofluorescence staining of α-SMA (green) and Col1A1 (red) of HPDFs (untreated, TGF-β1-treated, or TGF-β1-treated with the presence of 50 µM of AP) at 48 h. Nuclei were counterstained with DAPI (blue). Figure A(a–d) present the untreated cells showing α-SMA staining, Col1A1staining, DAPI staining, and merged images, respectively. Figure A(e–h) present TGF-β1-treated cells showing α-SMA staining, Col1A1staining, DAPI staining, and merged images, respectively. Figure A(i–l) present cells treated with TGF-β1 and 50 µM of AP showing α-SMA staining, Col1A1staining, DAPI staining, and merged images, respectively. (B) Western blot analysis showing dose-dependent effects of AP on reducing the TGF-β1-induced expression of α-SMA and Col1A1. β-Actin was used as a loading control and for normalization. (C) Quantitative analysis of the Western blot band intensity of α-SMA and Col1A1 of HPDFs (untreated, TGF-β1-treated, or TGF-β1-treated with the presence of 25 and 50 µM of AP) at 48 h. Scale bar = 500 µm. * p < 0.05 compared to TGF-β1-treated HPDFs.

Article Snippet: In some experiments, filamentous actin (F-actin) was stained with DyLight-594Phalloidin (catalog number12877) (Cell Signaling Technology, Boston, MA, USA).

Techniques: Expressing, Staining, Western Blot, Control