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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Nanobodies for the Early Detection of Ovarian Cancer
doi: 10.3390/ijms232213687
Figure Lengend Snippet: Expression levels of the nanobodies targeting HE4, SLPI, and PGRN and equilibrium dissociation constants (K D ), as measured by indirect ELISA and expressed by the EC50 value. Clones expressed at levels >25 mg/L and showing a K D < 50 nM were further studied using SPR to assess their binding affinity.
Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and
Techniques: Expressing, Indirect ELISA, Clone Assay, Binding Assay
Journal: International Journal of Molecular Sciences
Article Title: Nanobodies for the Early Detection of Ovarian Cancer
doi: 10.3390/ijms232213687
Figure Lengend Snippet: Indirect ELISA results showing the antigen-binding capacities of the nanobody variants targeting HE4 ( top ), SLPI ( middle ), and PGRN ( bottom ).
Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and
Techniques: Indirect ELISA, Binding Assay
Journal: International Journal of Molecular Sciences
Article Title: Nanobodies for the Early Detection of Ovarian Cancer
doi: 10.3390/ijms232213687
Figure Lengend Snippet: Antigen-binding affinity of Nbs HE4-1 ( top ), SLPI-7 ( middle ), and PGRN-7 ( bottom ), as determined by SPR (k on in M −1 .s −1 and k off in s −1 ).
Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and
Techniques: Binding Assay
Journal: International Journal of Molecular Sciences
Article Title: Nanobodies for the Early Detection of Ovarian Cancer
doi: 10.3390/ijms232213687
Figure Lengend Snippet: Non-competitive Nb pair possibilities for SLPI (C—competitive; P.C—partially competitive; N.C—non-competitive).
Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and
Techniques:
Journal: International Journal of Molecular Sciences
Article Title: Nanobodies for the Early Detection of Ovarian Cancer
doi: 10.3390/ijms232213687
Figure Lengend Snippet: Sandwich ELISA plots showing the detection results of the recombinant human antigens HE4, SLPI, and PGRN by their respective best-performing nanobodies: HE4-6 in PBS ( A ) and serum ( B ); SLPI-1 in PBS ( C ) and serum ( D ); and PGRN-5 in PBS ( E ) and serum ( F ). The yellow line indicates the LOD.
Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and
Techniques: Sandwich ELISA, Recombinant
Journal: International Journal of Molecular Sciences
Article Title: Nanobodies for the Early Detection of Ovarian Cancer
doi: 10.3390/ijms232213687
Figure Lengend Snippet: Comparison between the serum levels in healthy women and the obtained limits of detection (LOD) for the recombinant human HE4, SLPI, and PGRN antigens by the selected nanobodies HE4-6, SLPI-1, and PGRN-5, respectively.
Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and
Techniques: Comparison, Recombinant
Journal: International Journal of Molecular Sciences
Article Title: Nanobodies for the Early Detection of Ovarian Cancer
doi: 10.3390/ijms232213687
Figure Lengend Snippet: Antigen dilution series for each Nb (dilutions in PBS and human serum).
Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and
Techniques: Concentration Assay